Rabbit Recombinant Monoclonal UQCRH antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Images
![Western blot - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--western-blot-img103867.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115946.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65709.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111)")
![Flow Cytometry (Intracellular) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--flow-cytometry-intracellular-img58128.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img37560.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (AB249111)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Tested |
| Mouse | Predicted | Predicted | Not recommended | Predicted |
| Rat | Expected | Tested | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c.
Alternative names
Complex III subunit 6, Complex III subunit VIII, Cytochrome c1 non-heme 11 kDa protein, Mitochondrial hinge protein, Ubiquinol-cytochrome c reductase complex 11 kDa protein, UQCRH
Recommended products
Rabbit Recombinant Monoclonal UQCRH antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9038(B)
- Purification technique
- Affinity purification Protein A
- Specificity
The immunogen used for this product shares 100% homology with UQCRHL. Cross-reactivity with this protein has not been confirmed experimentally.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249111 is the carrier-free version of Anti-UQCRH antibody [EPR9038(B)] ab154803.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UQCRH also known as ubiquinol-cytochrome c reductase hinge protein plays an important role in the electron transport chain. It forms a part of the core structure of cytochrome bc1 complex (Complex III) in mitochondria. UQCRH has a molecular mass of about 9 kDa and is expressed ubiquitously in human tissues. Its primary role is to assist in transferring electrons by connecting cytochrome c1 and cytochrome c which is essential for cellular respiration and energy production.
- Biological function summary
The hinge protein acts as a critical component of the mitochondrial respiratory chain complex III. UQCRH ensures the efficient transfer of electrons within the complex which contributes to generating a proton gradient across the inner mitochondrial membrane. This proton gradient drives ATP synthesis. It is a vital component of the larger respiratory chain complex which is important for aerobic cellular energy metabolism and plays an important role in overall mitochondrial function.
- Pathways
UQCRH is a significant player in the oxidative phosphorylation pathway which is central to the production of ATP. The protein closely interacts with components of Complex III and adjacent complexes of the electron transport chain such as NADH dehydrogenase (Complex I) and cytochrome c oxidase (Complex IV). These interactions help UQCRH integrate smoothly within the pathway ensuring the consistent flow of electrons and maintenance of ATP production.
- Associated diseases and disorders
UQCRH mutations or dysfunctions link to mitochondrial disorders such as mitochondrial complex III deficiency. Dysfunction in this protein disrupts normal mitochondrial respiration leading to various symptoms including myopathy and encephalopathy. Additionally impaired UQCRH function can contribute to the development of neurodegenerative diseases. Studies have observed connections between abnormal UQCRH activity and proteins such as superoxide dismutase-1 (SOD1) in disease states indicating its possible role in oxidative stress-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--western-blot-img103867.jpg "Western blot - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)")
Western blot - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
This data was developed using Anti-UQCRH antibody [EPR9038(B)] ab154803, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-UQCRH antibody [EPR9038(B)] (Anti-UQCRH antibody [EPR9038(B)] ab154803) at 1/1000 dilution
Lane 1: Fetal liver lysate at 10 µg
Lane 2: PC12 lysate at 10 µg
Lane 3: C6 lysate at 10 µg
Lane 4: Fetal brain lysate at 10 µg
Lane 5: Human spleen lysate at 10 µg
Lane 6: Saos 2 lysate at 10 µg
Predicted band size: 11 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115946.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
This data was developed using Anti-UQCRH antibody [EPR9038(B)] ab154803, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UQCRH with Anti-UQCRH antibody [EPR9038(B)] ab154803 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65709.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
This data was developed using Anti-UQCRH antibody [EPR9038(B)] ab154803, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRH with Anti-UQCRH antibody [EPR9038(B)] ab154803 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--flow-cytometry-intracellular-img58128.jpg "Flow Cytometry (Intracellular) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)")
Flow Cytometry (Intracellular) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
This data was developed using Anti-UQCRH antibody [EPR9038(B)] ab154803, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized Saos 2 cells labeling UQCRH with Anti-UQCRH antibody [EPR9038(B)] ab154803 at 1/10 dilution (red) or a rabbit IgG (green- negative control).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img37560.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
This data was developed using Anti-UQCRH antibody [EPR9038(B)] ab154803, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal brain tissue using Anti-UQCRH antibody [EPR9038(B)] ab154803 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133109.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
This data was developed using Anti-UQCRH antibody [EPR9038(B)] ab154803, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using Anti-UQCRH antibody [EPR9038(B)] ab154803 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119382.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
This data was developed using Anti-UQCRH antibody [EPR9038(B)] ab154803, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using Anti-UQCRH antibody [EPR9038(B)] ab154803 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133109.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111), expandable thumbnail](https://content.abcam.com/products/images/uqcrh-antibody-epr9038b-bsa-and-azide-free-ab249111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119382.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)")
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