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AB250403

Anti-URB2 antibody [EPR13404-35] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal URB2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0133, URB2, Unhealthy ribosome biogenesis protein 2 homolog

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-URB2 antibody [EPR13404-35] - BSA and Azide free (AB250403)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-URB2 antibody [EPR13404-35] - BSA and Azide free (AB250403)

This data was developed using ab181177, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 293 cells (paraformaldehyde-fixed, 4%) labeling URB2 with ab181177 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot - Anti-URB2 antibody [EPR13404-35] - BSA and Azide free (AB250403)
  • WB

Supplier Data

Western blot - Anti-URB2 antibody [EPR13404-35] - BSA and Azide free (AB250403)

This data was developed using ab181177, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-URB2 antibody [EPR13404-35] (<a href='/en-us/products/primary-antibodies/urb2-antibody-epr13404-35-ab181177'>ab181177</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Lane 4:

293 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 171 kDa

Observed band size: 171 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13404-35

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250403 is the carrier-free version of ab181177.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

URB2 also known as Utp23 is a protein with an approximate mass of 42 kDa. It functions as a nuclease that is essential for the maturation of small nucleolar ribonucleoprotein (snoRNP) complexes. URB2 is found primarily in the nucleolus where it plays a role in processing and modifying ribosomal RNA (rRNA). This protein is integral for the correct assembly of ribosome subunits impacting the overall efficiency and accuracy of protein synthesis.
Biological function summary

URB2 is involved in the ribosome biogenesis process which is a fundamental cellular activity. It participates as a part of the small-subunit (SSU) processome a large ribonucleoprotein complex responsible for the early steps of processing pre-18S rRNA. Through interaction with other nucleases and RNA helicases within the SSU processome URB2 ensures the precise cleavage of rRNA facilitating the formation of functional ribosomes. This activity is directly linked to cellular growth and proliferation.

Pathways

URB2 plays a significant role in the ribosome biosynthesis pathway. It interacts closely with other proteins such as Utp21 and Utp4 both important for the assemble of the SSU processome. The efficient ribosome synthesis pathway is stringent on the function of URB2 because defects in ribosomal assembly can slow down protein production affecting cellular growth. Additionally URB2 is associated with the nucleolar surveillance pathway which monitors ribosome assembly and manages stress responses in the nucleolus.

Altered URB2 function can contribute to ribosomopathies which are disorders resulting from defects in ribosome synthesis. Such defects in URB2 have been linked to conditions like Diamond-Blackfan anemia where dysfunctional ribosomal assembly leads to inadequate red blood cell production. URB2 is also associated with Treacher Collins syndrome through interactions with the TCOF1 protein impacting craniofacial development. Understanding URB2’s role in these conditions might offer insights into novel therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for hematopietic stem cell development through the regulation of p53/TP53 pathway.
See full target information URB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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