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AB34806

Anti-Urease antibody

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(3 Publications)

Rabbit Polyclonal UREA antibody. Suitable for ELISA and reacts with Canavalia ensiformis samples. Cited in 3 publications.

View Alternative Names

Urease, Jack bean urease, Urea amidohydrolase, JBU

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Canavalia ensiformis

Applications

ELISA

applications

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Complementary Products

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Primary Antibodies

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Primary Antibodies

AB221547

Anti-ZO1 tight junction protein antibody [EPR19945-296]

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Assay Kits

AB65349

NADP/NADPH Assay Kit

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Canavalia ensiformis": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Purification notes
Anti-Urease (Jack Bean) (Rabbit) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.164% Sodium phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Urease also known as urea amidohydrolase is an enzyme with a significant role in nitrogen metabolism. It mechanically catalyzes the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is commonly associated with a molecular mass ranging from 480 kDa to 600 kDa depending on species and isoform. Urease expresses predominantly in bacteria fungi and plants with significant incidence in gastrointestinal tract microorganisms like Helicobacter pylori. It is also present in some invertebrates but not usually in vertebrates.
Biological function summary

Urease plays a significant role in nitrogen metabolism by assisting the recycling of nitrogenous waste products and facilitating nutrient acquisition. It functions mainly as part of a multi-subunit complex usually composed of alpha beta and gamma subunits. These subunits coordinate to form a metalloenzyme that requires nickel ions to be catalytically active. This enzyme's ability to convert urea into ammonia makes it essential for nitrogen economy in various ecological settings.

Pathways

Urease is an integral component of the nitrogen cycle and is active in the urea degradation pathway. This pathway is important for recycling nitrogen in nature affecting soil fertility and plant growth. Urease links to proteins involved in ammonia assimilation such as glutamine synthetase which helps incorporate ammonia into amino acids. This relationship facilitates nitrogen availability for living organisms and enhances ecosystem productivity.

Urease relates to conditions like gastric ulcers and certain kidney stones. Helicobacter pylori employs urease to neutralize stomach acid enabling its survival and leading to gastritis and peptic ulcer disease. Also urease activity contributes to the formation of struvite stones in the urinary tract where urea-splitting bacteria like Proteus mirabilis produce ammonia leading to stone formation. These conditions illustrate urease’s involvement with bacterial proteins like flagellar proteins and virulence factors that facilitate colonization and infection.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism (PubMed : 26690979). Is known to be highly toxic and lethal when given by intravenous route, producing convulsions and other signs of central nervous system intoxication associated with the high levels of ammonia formed in the blood of mice and rabbits (PubMed : 26690979). Is neurotoxic in mammals, when directly injected into hippocampus (PubMed : 33631299). It may induce seizures by acting at a neuronal network level, thereby disturbing electroencephalographic rhythms and causing metabolic alterations in key areas related to epileptogenesis and to neurogenic pulmonary edema (PubMed : 33631299). It increases calcium influx and neuronal firing rate in the hippocampus (PubMed : 33631299). Is able to insert itself into lipid bilayers, altering physicochemical properties of artificial membranes, and forming cation-selective ion channels (PubMed : 24583269). In vitro, has the ability to induce platelet aggregation, platelet granules secretion and release of ATP (PubMed : 11696010). In contrast to canatoxin, another urease from C.ensiformis, is not lethal to mice when intraperitoneally injected (PubMed : 11696010).
See full target information Urease
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2206893 PubMed36775865

2023

Metabolic Modulation of Intracellular Ammonia via Intravesical Instillation of Nanoporter-Encased Hydrogel Eradicates Bladder Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqiang Jing,Chen Chen,Ganyu Wang,Maosen Han,Shouzhen Chen,Xin Jiang,Chongdeng Shi,Peng Sun,Zhenmei Yang,Benkang Shi,Xinyi Jiang

Phytomedicine : international journal of phytother 65:153097 PubMed31568921

2019

Effect of patchouli alcohol on macrophage mediated Helicobacter pylori digestion based on intracellular urease inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

D W Lian,Y F Xu,Q H Deng,X M Lin,B Huang,S X Xian,P Huang

Journal of lipid research 51:3126-34 PubMed20724654

2010

Interaction of phospholipid transfer protein with human tear fluid mucins.

Applications

Unspecified application

Species

Unspecified reactive species

Niko L Setälä,Juha M Holopainen,Jari Metso,Gebrenegus Yohannes,Jaakko Hiidenhovi,Leif C Andersson,Ove Eriksson,Alexandra Robciuc,Matti Jauhiainen
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Product promise

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