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AB127916

Anti-ureB antibody

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(5 Publications)

Rabbit Polyclonal ureB antibody. Suitable for IHC-P, WB and reacts with Helicobacter pylori samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Helicobacter pylori 26695 ureB aa 300-400.

View Alternative Names

hpuB, HP_0072, ureB, Urease subunit beta, Urea amidohydrolase subunit beta

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ureB antibody (AB127916)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ureB antibody (AB127916)

ab127916, at 1/500 dilution, staining ureB in paraffin-embedded Mice Gastric pylori, by Immunohistochemistry.

Western blot - Anti-ureB antibody (AB127916)
  • WB

Unknown

Western blot - Anti-ureB antibody (AB127916)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ureB antibody (ab127916) at 1/50000 dilution

Lane 1:

ureB at 0.5 µg

Lane 2:

ureB at 1 µg

Predicted band size: 62 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Helicobacter pylori

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Helicobacter pylori 26695 ureB aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

P69996

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Helicobacter pylori": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>citrate buffer-10mM</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "<p></p>" } } }

Product details

Keep as concentrated solution.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.
See full target information ureB

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:14185 PubMed38902391

2024

A prominent role of LncRNA H19 in H. pylori CagA induced DNA damage response and cell malignancy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofeng He,Tingting Huang,Qinrong Wang,Liya Bao,Zhengrong Wang,Hui Song,Yanhong Li,Jianjiang Zhou,Yan Zhao,Yuan Xie

Vaccine 38:3031-3041 PubMed32139315

2020

Oral immunization of mice with a multivalent therapeutic subunit vaccine protects against Helicobacter pylori infection.

Applications

Unspecified application

Species

Unspecified reactive species

Meiying Liu,Youxiu Zhong,Jing Chen,Yu Liu,Chongfa Tang,Xuewei Wang,Yanbin Zhang,Ping Wang,Susan M Logan,Wangxue Chen,Bo Wei

Cancers 11: PubMed31185594

2019

The Urease Virulence Factor Is Required for the Induction of Hypoxia-Induced Factor-1α in Gastric Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Valenzuela-Valderrama,Paulina Cerda-Opazo,Steffen Backert,María Fernanda González,Nicolás Carrasco-Véliz,Carla Jorquera-Cordero,Sergio Wehinger,Jimena Canales,Denisse Bravo,Andrew F G Quest

Journal of experimental & clinical cancer research : CR 37:280 PubMed30466467

2018

Helicobacter pylori CagA promotes epithelial mesenchymal transition in gastric carcinogenesis via triggering oncogenic YAP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nianshuang Li,Yan Feng,Yi Hu,Cong He,Chuan Xie,Yaobin Ouyang,Stephen C Artim,Deqiang Huang,Yin Zhu,Zhijun Luo,Zhongming Ge,Nonghua Lu

Helicobacter 23:e12470 PubMed29488678

2018

Monotherapy with a novel intervenolin derivative, AS-1934, is an effective treatment for Helicobacter pylori infection.

Applications

WB

Species

Unspecified reactive species

Tomokazu Ohishi,Tohru Masuda,Hikaru Abe,Chigusa Hayashi,Hayamitsu Adachi,Shun-Ichi Ohba,Masayuki Igarashi,Takumi Watanabe,Hitomi Mimuro,Eri Amalia,Daniel Ken Inaoka,Kota Mochizuki,Kiyoshi Kita,Masakatsu Shibasaki,Manabu Kawada
View all publications

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