AB220490

Anti-Urm1 antibody

Name: Anti-Urm1 antibody (ab220490) | Abcam
Brand: Abcam Limited
SKU: AB220490

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(2 Publications)

Rabbit Polyclonal URM1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human URM1 aa 1-100.

View Alternative Names

C9orf74, URM1, Ubiquitin-related modifier 1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Urm1 antibody (AB220490)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Urm1 antibody (AB220490)

Immunofluorescent analysis of MCF7 cells (PFA-fixed/Triton X-100 permeabilized) labeling Urm1 with ab220490 at 4 μg/mL (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human URM1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BTM9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Urm1 also known as ubiquitin-related modifier 1 is a small protein with a mass of approximately 11 kDa. It belongs to the ubiquitin-like protein family and is expressed in a wide range of tissues notably in human tissues at the cellular level. Urm1 functions as a molecular tag influencing a variety of cellular processes through its conjugation to substrate proteins. It shares structural similarities with ubiquitin and employs a similar conjugation pathway involving E1-like activating enzymes.

Biological function summary

Urm1 acts in the thiolation of tRNA and as part of the ubiquitin-related modifier system which affects protein modification and signaling. It plays roles in regulating sulfur metabolism and is important in the biosynthesis of thiolated tRNAs. Urm1 is also involved in oxidative stress response by being part of a complex that modifies proteins in reaction to environmental stressors. As a result Urm1 impacts cellular homeostasis and the stability of various proteins within the cell.

Pathways

Urm1 influences the tRNA modification pathway an important mechanism for proper protein synthesis and function. It also participates in the cellular response to oxidative stress primarily through its interaction with cellular signaling proteins such as ROS-responsive transcription factors. In these pathways Urm1-related conjugations modify proteins affecting their activity during cellular defense mechanisms and the maintenance of protein structure under stress conditions.

Urm1 impacts conditions related to cellular redox states such as cancer and neurodegenerative disorders. Aberrant regulation of Urm1 and its pathways can lead to increased oxidative stress and faulty protein synthesis contributing to these diseases. Urm1's interaction with other proteins such as p53 in tumorigenesis highlights its relevance in the cellular processes that when deregulated can lead to cancer progression. In neurodegenerative diseases diminished Urm1 function can result in the accumulation of misfolded proteins exacerbating disease phenotypes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.

See full target information URM1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2205395 PubMed36594618

2023

Combined Single-Cell and Spatial Transcriptomics Reveal the Metabolic Evolvement of Breast Cancer during Early Dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ming Liu,Jing-Yu Ge,Yu-Fei Chen,Tong Liu,Lie Chen,Cui-Cui Liu,Ding Ma,Yi-Yu Chen,Yu-Wen Cai,Ying-Ying Xu,Zhi-Ming Shao,Ke-Da Yu

Autophagy 18:1763-1784 PubMed34836490

2021

HYPK coordinates degradation of polyneddylated proteins by autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Debasish Kumar Ghosh,Akash Ranjan

View all publications

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Product promise

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Anti-Urm1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Urm1 antibody (AB220490)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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