AB110699

Anti-Urm1 antibody [EPR4809]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal URM1 antibody. Suitable for WB and reacts with Human, Recombinant fragment samples.

View Alternative Names

C9orf74, URM1, Ubiquitin-related modifier 1

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Western blot - Anti-Urm1 antibody [EPR4809] (AB110699)

All lanes:

Western blot - Anti-Urm1 antibody [EPR4809] (ab110699) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

A549 lysate at 10 µg

Lane 3:

Human fetal lung lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Lane 5:

DDDDK-tagged Urm1 recombinant protein at 0.01 µg

Predicted band size: 11 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4809

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Urm1 also known as ubiquitin-related modifier 1 is a small protein with a mass of approximately 11 kDa. It belongs to the ubiquitin-like protein family and is expressed in a wide range of tissues notably in human tissues at the cellular level. Urm1 functions as a molecular tag influencing a variety of cellular processes through its conjugation to substrate proteins. It shares structural similarities with ubiquitin and employs a similar conjugation pathway involving E1-like activating enzymes.

Biological function summary

Urm1 acts in the thiolation of tRNA and as part of the ubiquitin-related modifier system which affects protein modification and signaling. It plays roles in regulating sulfur metabolism and is important in the biosynthesis of thiolated tRNAs. Urm1 is also involved in oxidative stress response by being part of a complex that modifies proteins in reaction to environmental stressors. As a result Urm1 impacts cellular homeostasis and the stability of various proteins within the cell.

Pathways

Urm1 influences the tRNA modification pathway an important mechanism for proper protein synthesis and function. It also participates in the cellular response to oxidative stress primarily through its interaction with cellular signaling proteins such as ROS-responsive transcription factors. In these pathways Urm1-related conjugations modify proteins affecting their activity during cellular defense mechanisms and the maintenance of protein structure under stress conditions.

Urm1 impacts conditions related to cellular redox states such as cancer and neurodegenerative disorders. Aberrant regulation of Urm1 and its pathways can lead to increased oxidative stress and faulty protein synthesis contributing to these diseases. Urm1's interaction with other proteins such as p53 in tumorigenesis highlights its relevance in the cellular processes that when deregulated can lead to cancer progression. In neurodegenerative diseases diminished Urm1 function can result in the accumulation of misfolded proteins exacerbating disease phenotypes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.

See full target information URM1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Urm1 antibody [EPR4809]

Western blot - Anti-Urm1 antibody [EPR4809] (AB110699)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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