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Rabbit Recombinant Monoclonal Urocortin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Urocortin antibody [EPR25060-64] (AB283503), expandable thumbnail
  • Western blot - Anti-Urocortin antibody [EPR25060-64] (AB283503), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (AB283503), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (AB283503), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (AB283503), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-FrIHC-P
Human
Not recommended
Tested
Not recommended
Not recommended
Tested
Mouse
Not recommended
Tested
Not recommended
Not recommended
Tested
Rat
Not recommended
Tested
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) (PubMed:8612563). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta (PubMed:8612563). Plays a role in the establishment of normal hearing thresholds (By similarity). Reduces food intake and regulates ghrelin levels in gastric body and plasma (By similarity).

Alternative names

Urocortin, UCN

Recommended products

Rabbit Recombinant Monoclonal Urocortin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25060-64

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Urocortin also known as UCN is a member of the corticotropin-releasing factor (CRF) family of peptides with a molecular weight of approximately 4.7 kDa. It engages in binding to CRF receptors especially CRF-R2 promoting a range of effects in different tissues. Urocortin is expressed in numerous brain regions including the hypothalamus and Edinger-Westphal nucleus as well as in peripheral tissues like the gastrointestinal tract and heart.

Biological function summary

This peptide hormone is involved in stress response and regulation of food intake. Urocortin modulates the activity of neuronal systems contributing to various physiological functions including cardiovascular homeostasis. It does not work as part of a complex but exerts its effects through receptor-ligand interactions predominantly with CRF-R2 influencing vasodilation and reducing anxiety-like behaviors.

Pathways

Urocortin exerts its biological roles through the corticotropin-releasing hormone signaling pathway. This pathway is fundamental in the body's response to stress. Urocortin's interaction with CRF-R2 links it to other CRF-related peptides such as CRF itself and Urocortin II in regulating stress-related responses and energy homeostasis via these pathways.

Associated diseases and disorders

Research indicates Urocortin's involvement in heart failure and anxiety disorders. In heart failure Urocortin's role in cardiovascular function places it alongside proteins such as atrial natriuretic peptide (ANP) in maintaining heart health. Moreover in anxiety disorders it shares connections with CRF in modulating behavioral responses to stress pointing to potential therapeutic applications in treating stress-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-Urocortin antibody [EPR25060-64] (ab283503), expandable thumbnail

    Western blot - Anti-Urocortin antibody [EPR25060-64] (ab283503)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10722688, 11788672).

    No expression was observed in the kidney.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-Urocortin antibody [EPR25060-64] (ab283503) at 1/1000 dilution

    Lane 1: Human heart tissue lysate at 20 µg

    Lane 2: Human kidney tissue lysate at 20 µg

    Lane 3: Mouse heart tissue lysate at 20 µg

    Lane 4: Mouse kidney tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution; Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution.

    Predicted band size: 13 kDa

    Observed band size: 19 kDa

  • Western blot - Anti-Urocortin antibody [EPR25060-64] (ab283503), expandable thumbnail

    Western blot - Anti-Urocortin antibody [EPR25060-64] (ab283503)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    All lanes: Western blot - Anti-Urocortin antibody [EPR25060-64] (ab283503) at 1/1000 dilution

    All lanes: Rat heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 13 kDa

    Observed band size: 19 kDa

    This data was developed using ab283503, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human cardiac muscle (PMID: 11788672).The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503)

    Immunohistochemical analysis of paraffin-embedded Mouse midbrain tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Edinger-Westphal nucleus in mouse midbrain (PMID: 12091910, PMID: 18186029).The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503)

    Immunohistochemical analysis of paraffin-embedded Rat midbrain tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Edinger-Westphal nucleus in rat midbrain (PMID: 12091910, PMID: 18186029).The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on rat cardiac muscle (PMID: 11788672).The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human kideny.The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: no staining on mouse kidney.The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] (ab283503)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on rat kidney.The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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