AB283516

Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Urocortin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

View Alternative Names

Urocortin, UCN

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

This data was developed using ab283503, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cardiac muscle (PMID : 11788672).The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

This data was developed using ab283503, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on human kideny.The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

This data was developed using ab283503, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cardiac muscle (PMID : 11788672).The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

This data was developed using ab283503, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : no staining on mouse kidney.The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

This data was developed using ab283503, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat midbrain tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Edinger-Westphal nucleus in rat midbrain (PMID : 12091910, PMID : 18186029).The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

This data was developed using ab283503, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse midbrain tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Edinger-Westphal nucleus in mouse midbrain (PMID : 12091910, PMID : 18186029).The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

This data was developed using ab283503, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling Urocortin with ab283503 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on rat kidney.The section was incubated with ab283503 at 4°C overnight Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Lab

Western blot - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

This data was developed using ab283503, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 10722688, 11788672).

No expression was observed in the kidney.

Exposure time : 3 minutes.

No expression was observed in the kidney.

3 minutes

Exposure time :

All lanes:

Western blot - Anti-Urocortin antibody [EPR25060-64] (<a href='/en-us/products/primary-antibodies/urocortin-antibody-epr25060-64-ab283503'>ab283503</a>) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution; Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution.

Predicted band size: 13 kDa

Observed band size: 19 kDa

false

Lab

Western blot - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

This data was developed using ab283503, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds.

All lanes:

Western blot - Anti-Urocortin antibody [EPR25060-64] (<a href='/en-us/products/primary-antibodies/urocortin-antibody-epr25060-64-ab283503'>ab283503</a>) at 1/1000 dilution

All lanes:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 13 kDa

Observed band size: 19 kDa

false

Unconjugated

Anti-Urocortin antibody [EPR25060-64]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25060-64

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat, Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab283516 is the carrier-free version of ab283503.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Urocortin also known as UCN is a member of the corticotropin-releasing factor (CRF) family of peptides with a molecular weight of approximately 4.7 kDa. It engages in binding to CRF receptors especially CRF-R2 promoting a range of effects in different tissues. Urocortin is expressed in numerous brain regions including the hypothalamus and Edinger-Westphal nucleus as well as in peripheral tissues like the gastrointestinal tract and heart.

Biological function summary

This peptide hormone is involved in stress response and regulation of food intake. Urocortin modulates the activity of neuronal systems contributing to various physiological functions including cardiovascular homeostasis. It does not work as part of a complex but exerts its effects through receptor-ligand interactions predominantly with CRF-R2 influencing vasodilation and reducing anxiety-like behaviors.

Pathways

Urocortin exerts its biological roles through the corticotropin-releasing hormone signaling pathway. This pathway is fundamental in the body's response to stress. Urocortin's interaction with CRF-R2 links it to other CRF-related peptides such as CRF itself and Urocortin II in regulating stress-related responses and energy homeostasis via these pathways.

Research indicates Urocortin's involvement in heart failure and anxiety disorders. In heart failure Urocortin's role in cardiovascular function places it alongside proteins such as atrial natriuretic peptide (ANP) in maintaining heart health. Moreover in anxiety disorders it shares connections with CRF in modulating behavioral responses to stress pointing to potential therapeutic applications in treating stress-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) (PubMed : 8612563). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta (PubMed : 8612563). Plays a role in the establishment of normal hearing thresholds (By similarity). Reduces food intake and regulates ghrelin levels in gastric body and plasma (By similarity).

See full target information Urocortin

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

Western blot - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

Western blot - Anti-Urocortin antibody [EPR25060-64] - BSA and Azide free (AB283516)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise