Rabbit Recombinant Monoclonal Urokinase antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Urokinase antibody [EPR20229-162] - BSA and Azide free (Detector) (AB242914), expandable thumbnail](https://content.abcam.com/products/images/urokinase-antibody-epr20229-162-bsa-and-azide-free-detector-ab242914--sandwich-elisa-img52787.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Urokinase antibody [EPR20229-162] - BSA and Azide free (Detector) (AB242914)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
Alternative names
Urokinase-type plasminogen activator, U-plasminogen activator, uPA, PLAU
Recommended products
Rabbit Recombinant Monoclonal Urokinase antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20229-162
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab242914 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Urokinase type Plasminogen Activator. The recommended pair for sandwich ELISA is:
Capture: Anti-Urokinase antibody [EPR20229-146] - BSA and Azide free (Capture) ab242669, Human Urokinase type Plasminogen Activator Capture Antibody (unconjugated)
Detector: ab242914, Human Urokinase type Plasminogen Activator Detector Antibody (unconjugated)
The reference range value is 31.3 - 2000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Urokinase also known as urokinase-type plasminogen activator (uPA) is a serine protease with a molecular mass of approximately 54 kDa. It is expressed in various tissues including the kidneys liver and blood vessels. Urokinase initially forms as pro-urokinase an inactive precursor that can convert into the active enzyme. Researchers often use assays like the direct assay to measure urokinase activity due to its role in breaking down fibrin and modulating cell adhesion and migration.
- Biological function summary
Something particularly interesting about urokinase is its role in converting plasminogen to plasmin a critical enzyme in the process of fibrinolysis. Moreover it is part of a larger enzymatic complex involving the urokinase receptor (uPAR) which localizes plasminogen activation to cell surfaces. This complex mediates extracellular matrix degradation aiding in important cellular processes like tissue remodeling and wound healing.
- Pathways
Urokinase plays a critical role in the fibrinolytic pathway which involves the breakdown of blood clots. It also interacts with the extracellular matrix pathway by activating plasminogen which can degrade matrix components. In these pathways urokinase closely interacts with proteins such as plasmin and its inhibitor plasminogen activator inhibitor-1 (PAI-1) which regulate the activity and localization of proteolytic events.
- Associated diseases and disorders
Urokinase is strongly linked to conditions such as deep vein thrombosis and cancer metastasis. Its overexpression or dysregulation often leads to excessive tissue degradation influencing metastasis progression. Urokinase is connected to the urokinase receptor (uPAR) in cancer facilitating cancer cell invasion and migration. Understanding its behavior in these contexts is critical for developing therapeutic strategies and targeting treatments against these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Urokinase antibody [EPR20229-162] - BSA and Azide free (Detector) (ab242914), expandable thumbnail](https://content.abcam.com/products/images/urokinase-antibody-epr20229-162-bsa-and-azide-free-detector-ab242914--sandwich-elisa-img52787.jpg "Sandwich ELISA - Anti-Urokinase antibody [EPR20229-162] - BSA and Azide free (Detector) (ab242914)")
Sandwich ELISA - Anti-Urokinase antibody [EPR20229-162] - BSA and Azide free (Detector) (ab242914)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human U-Plasminogen Activator ELISA Kit ab226904).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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