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AB28230

Anti-Urokinase antibody [H77A10]

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(4 Publications)

Mouse Monoclonal Urokinase antibody. Suitable for WB and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Urokinase-type plasminogen activator.

View Alternative Names

Urokinase-type plasminogen activator, U-plasminogen activator, uPA, Plau

1 Images
Western blot - Anti-Urokinase antibody [H77A10] (AB28230)
  • WB

Unknown

Western blot - Anti-Urokinase antibody [H77A10] (AB28230)

All lanes:

Western blot - Anti-Urokinase antibody [H77A10] (ab28230) at 3 µg/mL

Lane 1:

Mouse Urokinase (0.5ug)

Lane 2:

MUPA (0.5ug) + CPAI (2.5ug)

Lane 3:

Prestained standard

Predicted band size: 49 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

H77A10

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Urokinase-type plasminogen activator.

P06869

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

This antibody was produced in a knockout mouse.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 6.6 Constituents: 0.87% Sodium chloride, 0.82% Sodium phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Urokinase also known as urokinase-type plasminogen activator (uPA) is a serine protease with a molecular mass of approximately 54 kDa. It is expressed in various tissues including the kidneys liver and blood vessels. Urokinase initially forms as pro-urokinase an inactive precursor that can convert into the active enzyme. Researchers often use assays like the direct assay to measure urokinase activity due to its role in breaking down fibrin and modulating cell adhesion and migration.
Biological function summary

Something particularly interesting about urokinase is its role in converting plasminogen to plasmin a critical enzyme in the process of fibrinolysis. Moreover it is part of a larger enzymatic complex involving the urokinase receptor (uPAR) which localizes plasminogen activation to cell surfaces. This complex mediates extracellular matrix degradation aiding in important cellular processes like tissue remodeling and wound healing.

Pathways

Urokinase plays a critical role in the fibrinolytic pathway which involves the breakdown of blood clots. It also interacts with the extracellular matrix pathway by activating plasminogen which can degrade matrix components. In these pathways urokinase closely interacts with proteins such as plasmin and its inhibitor plasminogen activator inhibitor-1 (PAI-1) which regulate the activity and localization of proteolytic events.

Urokinase is strongly linked to conditions such as deep vein thrombosis and cancer metastasis. Its overexpression or dysregulation often leads to excessive tissue degradation influencing metastasis progression. Urokinase is connected to the urokinase receptor (uPAR) in cancer facilitating cancer cell invasion and migration. Understanding its behavior in these contexts is critical for developing therapeutic strategies and targeting treatments against these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
See full target information Urokinase-type plasminogen activator
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of chronic obstructive pulmonary disease 16:2817-2832 PubMed34675506

2021

GLIPR1 Protects Against Cigarette Smoke-Induced Airway Inflammation via PLAU/EGFR Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Peng,Yuanyuan Wu,Ge Zhang,Wensi Zhu,Meijia Chang,Ainiwaer Rouzi,Weipeng Jiang,Lin Tong,Qin Wang,Jie Liu,Yuanlin Song,Huayin Li,Ka Li,Jian Zhou

International journal of molecular sciences 22: PubMed33810260

2021

Reduced Expression of Urokinase Plasminogen Activator in Brown Adipose Tissue of Obese Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Chung-Ze Wu,Li-Chien Chang,Chao-Wen Cheng,Te-Chao Fang,Yuh-Feng Lin,Dee Pei,Jin-Shuen Chen

Diabetes 60:1964-72 PubMed21593201

2011

Inhibition of plasminogen activator inhibitor-1 restores skeletal muscle regeneration in untreated type 1 diabetic mice.

Applications

WB

Species

Mouse

Matthew P Krause,Jasmin Moradi,Aliyah A Nissar,Michael C Riddell,Thomas J Hawke

Cellular & molecular biology letters 16:101-13 PubMed21225469

2011

Ets-1 expression and gemcitabine chemoresistance in pancreatic cancer cells.

Applications

WB

Species

Human

Amit Khanna,Kulandaivelu Mahalingam,Debarshi Chakrabarti,Giridharan Periyasamy
View all publications
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Product promise

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