Anti-Uroplakin Ib/UPIb antibody [2N23]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777)

Immunohistochemical analysis of paraffin-embedded rat bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat kidney tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777)

Immunohistochemical analysis of paraffin-embedded rat bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.032μg/ml, followed by Anti-Mouse IgG2b antibody [RM108] (ab190482). Positive staining on rat bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature, followed by anti-mouse IgG2b IgG antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by Anti-Mouse IgG2b antibody [RM108] (ab190482).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777)

Immunohistochemical analysis of paraffin-embedded mouse bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.032μg/ml, followed by Anti-Mouse IgG2b antibody [RM108] (ab190482). Positive staining on mouse bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature, followed by anti-mouse IgG2b IgG antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by Anti-Mouse IgG2b antibody [RM108] (ab190482).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 3.94μg/ml, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at a 1/1000 dilution (Green). Positive staining on mouse kidney is observed. Nuclear counterstain is DAPI (Blue).
Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at a 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 1/50 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 3.94μg/ml (Green). Positive staining on rat kidney is observed. Nuclear counterstain is DAPI (Blue).
Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at a 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777)

Immunohistochemical analysis of paraffin-embedded mouse bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.