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Mouse Recombinant Monoclonal Uroplakin Ib/UPIb antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (AB237777), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrIHC-P
Mouse
Not recommended
Tested
Tested
Rat
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
3.94 µg/mL
Notes

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Rat
Dilution info
3.94 µg/mL
Notes

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Tested
Tested

Species
Mouse
Dilution info
0.03000-0.10000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
0.03000-0.10000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity).

Alternative names

Uroplakin-1b, UP1b, Uroplakin Ib, UPIb, Upk1b

Recommended products

Mouse Recombinant Monoclonal Uroplakin Ib/UPIb antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
2N23
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Uroplakin Ib (UPIb) also known as UPK1B is a type I integral membrane protein belonging to the uroplakin family. UPIb has a molecular mass of around 25 kDa. It is expressed in the apical surface of urothelial cells which line the urinary bladder and other parts of the urinary tract. The protein plays a structural role contributing to the formation of the asymmetrical unit membrane (AUM) an important component of the urothelial barrier.

Biological function summary

UPIb functions as part of a larger uroplakin complex essential for maintaining the urothelial surface structure. This protein works in concert with Uroplakin Ia Uroplakin II and Uroplakin III to form UPIa/UPIb heterodimers and facilitate protective plaque formation on the urothelial surface. The uroplakin complex ensures impermeability and mechanical stability in the urothelium critically protecting the underlying tissues from urine's toxic and osmotic effects.

Pathways

UPIb plays an essential role in the processes of cell differentiation and urothelial development. It interacts with transmembrane proteins within the uroplakin complex supporting the endocytosis and recycling pathways in the bladder's epithelial cells. The protein influences pathways pertinent to epithelial cell adhesion and communication working closely with uroplakin-associated proteins such as Uroplakin II and Uroplakin IIIa.

Associated diseases and disorders

UPIb is linked to bladder-related conditions due to its involvement in maintaining the urothelial barrier's integrity. Defects or misregulation in UPIb expression have associations with bladder cancer and interstitial cystitis. In bladder cancer abnormal uroplakin expression patterns including UPIb may indicate changes in the differentiation state of cancerous cells. In interstitial cystitis impaired urothelial barrier functions may link to UPIb's disrupted interactions with proteins such as Uroplakin IIIa.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on rat kidney tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 3.94μg/ml (Green). Positive staining on rat kidney is observed. Nuclear counterstain is DAPI (Blue).
    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 3.94μg/ml, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution (Green). Positive staining on mouse kidney is observed. Nuclear counterstain is DAPI (Blue).
    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on rat bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on mouse bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.032μg/ml, followed by Anti-Mouse IgG2b antibody [RM108] (Anti-Mouse IgG2b antibody [RM108] ab190482). Positive staining on rat bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature, followed by anti-mouse IgG2b IgG antibody (Anti-Mouse IgG2b antibody [RM108] ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Anti-Mouse IgG2b antibody [RM108] (Anti-Mouse IgG2b antibody [RM108] ab190482).

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.032μg/ml, followed by Anti-Mouse IgG2b antibody [RM108] (Anti-Mouse IgG2b antibody [RM108] ab190482). Positive staining on mouse bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature, followed by anti-mouse IgG2b IgG antibody (Anti-Mouse IgG2b antibody [RM108] ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Anti-Mouse IgG2b antibody [RM108] (Anti-Mouse IgG2b antibody [RM108] ab190482).

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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