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Mouse Recombinant Monoclonal Uroplakin Ib/UPIb antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (AB255743), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (AB255743), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (AB255743), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (AB255743), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (AB255743), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrIHC-P
Mouse
Not recommended
Tested
Tested
Rat
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
3.94 µg/mL
Notes

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Rat
Dilution info
3.94 µg/mL
Notes

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Tested
Tested

Species
Mouse
Dilution info
0.03000-0.10000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
0.03000-0.10000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity).

Alternative names

Recommended products

Mouse Recombinant Monoclonal Uroplakin Ib/UPIb antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
2N23
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab255743 is the carrier-free version of Anti-Uroplakin Ib/UPIb antibody [2N23] ab237777.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Uroplakin Ib (UPIb) also known as UPK1B is a type I integral membrane protein belonging to the uroplakin family. UPIb has a molecular mass of around 25 kDa. It is expressed in the apical surface of urothelial cells which line the urinary bladder and other parts of the urinary tract. The protein plays a structural role contributing to the formation of the asymmetrical unit membrane (AUM) an important component of the urothelial barrier.

Biological function summary

UPIb functions as part of a larger uroplakin complex essential for maintaining the urothelial surface structure. This protein works in concert with Uroplakin Ia Uroplakin II and Uroplakin III to form UPIa/UPIb heterodimers and facilitate protective plaque formation on the urothelial surface. The uroplakin complex ensures impermeability and mechanical stability in the urothelium critically protecting the underlying tissues from urine's toxic and osmotic effects.

Pathways

UPIb plays an essential role in the processes of cell differentiation and urothelial development. It interacts with transmembrane proteins within the uroplakin complex supporting the endocytosis and recycling pathways in the bladder's epithelial cells. The protein influences pathways pertinent to epithelial cell adhesion and communication working closely with uroplakin-associated proteins such as Uroplakin II and Uroplakin IIIa.

Associated diseases and disorders

UPIb is linked to bladder-related conditions due to its involvement in maintaining the urothelial barrier's integrity. Defects or misregulation in UPIb expression have associations with bladder cancer and interstitial cystitis. In bladder cancer abnormal uroplakin expression patterns including UPIb may indicate changes in the differentiation state of cancerous cells. In interstitial cystitis impaired urothelial barrier functions may link to UPIb's disrupted interactions with proteins such as Uroplakin IIIa.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com