AB183503

Anti-Uroplakin Ib/UPIb antibody [EPR14451]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Uroplakin Ib/UPIb antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

TSPAN20, UPK1B, Uroplakin-1b, UP1b, Tetraspanin-20, Uroplakin Ib, Tspan-20, UPIb

2 Images

Supplier Data

Western blot - Anti-Uroplakin Ib/UPIb antibody [EPR14451] (AB183503)

All lanes:

Western blot - Anti-Uroplakin Ib/UPIb antibody [EPR14451] (ab183503) at 1/20000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 25 kDa

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Supplier Data

Western blot - Anti-Uroplakin Ib/UPIb antibody [EPR14451] (AB183503)

All lanes:

Western blot - Anti-Uroplakin Ib/UPIb antibody [EPR14451] (ab183503) at 1/100000 dilution

All lanes:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14451

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Allow to warm to room temp and agitate gently before aliquoting

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uroplakin Ib (UPIb) also known as UPK1B is a type I integral membrane protein belonging to the uroplakin family. UPIb has a molecular mass of around 25 kDa. It is expressed in the apical surface of urothelial cells which line the urinary bladder and other parts of the urinary tract. The protein plays a structural role contributing to the formation of the asymmetrical unit membrane (AUM) an important component of the urothelial barrier.

Biological function summary

UPIb functions as part of a larger uroplakin complex essential for maintaining the urothelial surface structure. This protein works in concert with Uroplakin Ia Uroplakin II and Uroplakin III to form UPIa/UPIb heterodimers and facilitate protective plaque formation on the urothelial surface. The uroplakin complex ensures impermeability and mechanical stability in the urothelium critically protecting the underlying tissues from urine's toxic and osmotic effects.

Pathways

UPIb plays an essential role in the processes of cell differentiation and urothelial development. It interacts with transmembrane proteins within the uroplakin complex supporting the endocytosis and recycling pathways in the bladder's epithelial cells. The protein influences pathways pertinent to epithelial cell adhesion and communication working closely with uroplakin-associated proteins such as Uroplakin II and Uroplakin IIIa.

UPIb is linked to bladder-related conditions due to its involvement in maintaining the urothelial barrier's integrity. Defects or misregulation in UPIb expression have associations with bladder cancer and interstitial cystitis. In bladder cancer abnormal uroplakin expression patterns including UPIb may indicate changes in the differentiation state of cancerous cells. In interstitial cystitis impaired urothelial barrier functions may link to UPIb's disrupted interactions with proteins such as Uroplakin IIIa.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity).

See full target information UPK1B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 52:73-79 PubMed39278854

2024

Shengmai Powder regulates alveolar macrophage PPAR-γ and improves the chronic inflammatory state of chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Liu,Zongwei Liu,Xunxun Ma,Shengjie Wang,Jie Lin,Xiuyan Shi,Xiaoyong Xu

Cells 12: PubMed37681927

2023

Lymphatic Endothelial-to-Myofibroblast Transition: A Potential New Mechanism Underlying Skin Fibrosis in Systemic Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Rosa,Eloisa Romano,Bianca Saveria Fioretto,Khadija El Aoufy,Silvia Bellando-Randone,Marco Matucci-Cerinic,Mirko Manetti

Bioengineered 13:3958-3968 PubMed35112986

2022

Effect of α-tocopherol in alleviating the lipopolysaccharide-induced acute lung injury via inhibiting nuclear factor kappa-B signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Mu Hu,Jielai Yang,Yang Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Uroplakin Ib/UPIb antibody [EPR14451]

Western blot - Anti-Uroplakin Ib/UPIb antibody [EPR14451] (AB183503)

Western blot - Anti-Uroplakin Ib/UPIb antibody [EPR14451] (AB183503)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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