AB263454

Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081]

Name: Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (ab263454) | Abcam
Brand: Abcam Limited
SKU: AB263454
Price: 495 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal Uroplakin Ib/UPIb antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UPK1B aa 100-250.

View Alternative Names

TSPAN20, UPK1B, Uroplakin-1b, UP1b, Tetraspanin-20, Uroplakin Ib, Tspan-20, UPIb

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue stained for Uroplakin Ib/UPIb using ab263454 at 2 μg/ml in immunohistochemistry.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue stained for Uroplakin Ib/UPIb using ab263454 at 2 μg/ml in immunohistochemistry.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue stained for Uroplakin Ib/UPIb using ab263454 at 2 μg/ml in immunohistochemistry.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UPK1B/3081

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UPK1B aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

O75841

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with the primary antibody for 30 mins at RT.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uroplakin Ib (UPIb) also known as UPK1B is a type I integral membrane protein belonging to the uroplakin family. UPIb has a molecular mass of around 25 kDa. It is expressed in the apical surface of urothelial cells which line the urinary bladder and other parts of the urinary tract. The protein plays a structural role contributing to the formation of the asymmetrical unit membrane (AUM) an important component of the urothelial barrier.

Biological function summary

UPIb functions as part of a larger uroplakin complex essential for maintaining the urothelial surface structure. This protein works in concert with Uroplakin Ia Uroplakin II and Uroplakin III to form UPIa/UPIb heterodimers and facilitate protective plaque formation on the urothelial surface. The uroplakin complex ensures impermeability and mechanical stability in the urothelium critically protecting the underlying tissues from urine's toxic and osmotic effects.

Pathways

UPIb plays an essential role in the processes of cell differentiation and urothelial development. It interacts with transmembrane proteins within the uroplakin complex supporting the endocytosis and recycling pathways in the bladder's epithelial cells. The protein influences pathways pertinent to epithelial cell adhesion and communication working closely with uroplakin-associated proteins such as Uroplakin II and Uroplakin IIIa.

UPIb is linked to bladder-related conditions due to its involvement in maintaining the urothelial barrier's integrity. Defects or misregulation in UPIb expression have associations with bladder cancer and interstitial cystitis. In bladder cancer abnormal uroplakin expression patterns including UPIb may indicate changes in the differentiation state of cancerous cells. In interstitial cystitis impaired urothelial barrier functions may link to UPIb's disrupted interactions with proteins such as Uroplakin IIIa.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity).

See full target information UPK1B

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37958809

2023

mRNA and Protein Expression in Human Fetal Membrane Cells: Potential Biomarkers for Preterm Prelabor Rupture of the Fetal Membranes?

Applications

Unspecified application

Species

Unspecified reactive species

Emmeli Mikkelsen,Berthold Huppertz,Ripudaman Singh,Katarina Ravn,Lotte Hatt,Mogens Kruhøffer,Rheanna Urrabaz-Garza,Niels Uldbjerg,Ramkumar Menon,Torben Steiniche

Cancer research 69:8111-9 PubMed19808967

2009

Runt-related transcription factor RUNX3 is a target of MDM2-mediated ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Zi Chi,Jiyeon Kim,Yong-Hee Lee,Jung-Won Lee,Kyeong-Sook Lee,Heejun Wee,Wun-Jae Kim,Woo-Yoon Park,Byung-Chul Oh,Gary S Stein,Yoshiaki Ito,Andre J van Wijnen,Suk-Chul Bae

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise