AB224261

Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Uroplakin II/UPII antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Uroplakin-2, UP2, Uroplakin II, UPII, UPK2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (AB224261)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (AB224261)

Immunohistochemical analysis of paraffin-embedded human ureter tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and weak membrane staining on epithelial cells of human ureter tissues is observed [PMID : 24978921]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213655).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (AB224261)

This IHC data was generated using the same anti-Uroplakin II/UPII antibody clone [EPR18799] in a different buffer formulation (cat# ab213655).

Immunohistochemical analysis of paraffin-embedded human bladder tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on epithelial cells of human bladder tissues is observed [PMID : 24978921]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (AB224261)

Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control :No staining on tumor cells of human renal clear cell carcinoma. [PMID : 24978921].

Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213655).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (AB224261)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on tumor cells of human bladder cancer is observed [PMID : 24978921]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213655).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unconjugated

Anti-Uroplakin II/UPII antibody [EPR18799]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18799

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

ab224261 is the carrier-free version of ab213655.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uroplakin II (UPII) also known as UPK2 plays a structural role in the epithelial cells of the urinary tract. It has a molecular mass of approximately 15 kDa. UPII is expressed specifically in the urothelium which lines the interior surface of the bladder ureters and renal pelvis. This protein contributes to the asymmetric unit membrane (AUM) which forms specialized plaques essential for maintaining the impermeability of the urothelial barrier.

Biological function summary

Uroplakin II functions as part of the larger uroplakin protein complex. UPII interacts with other uroplakins such as Uroplakin Ia Ib and III contributing to the formation of a multimeric structure that strengthens the urothelial cells' surface. This structure plays a role in cellular stabilization and preventing the passage of toxic substances and pathogens from the urine into the underlying tissue. Consequently it aids in maintaining tissue integrity and ensuring efficient functioning of the urinary system.

Pathways

The uroplakin complex integrates into the pathway of epithelial differentiation. Uroplakin II's association with other uroplakins influences the formation of the AUM which involves the coordinated action of several related proteins such as claudins. The interaction with claudins suggests a potential regulatory role for cell-cell adhesion within epithelial transport pathways. This role might interact with tight junction pathways that are important for maintaining the selective permeability of epithelial layers.

Alterations in Uroplakin II expression link to urothelial carcinoma. Abnormal UPII levels can disrupt the urothelial barrier potentially leading to carcinogenesis. Investigations find connections between UPII and overexpression of proteins such as EGFR which contributes to tumor growth and progression. The association of UPII with bladder cancer emphasizes its potential as a diagnostic biomarker and a target for therapeutic strategies in urothelial malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in regulating the assembly of the AUM (By similarity).

See full target information Uroplakin-2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6446 PubMed37833281

2023

Rewiring of the promoter-enhancer interactome and regulatory landscape in glioblastoma orchestrates gene expression underlying neurogliomal synaptic communication.

Applications

Unspecified application

Species

Unspecified reactive species

Chaitali Chakraborty,Itzel Nissen,Craig A Vincent,Anna-Carin Hägglund,Andreas Hörnblad,Silvia Remeseiro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (AB224261)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (AB224261)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (AB224261)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (AB224261)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise