AB231576

Anti-Uroplakin III antibody

Name: Anti-Uroplakin III antibody (ab231576) | Abcam
Brand: Abcam Limited
SKU: AB231576
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal Uroplakin III antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

UPK3, UPK3A, Uroplakin-3a, UP3a, Uroplakin III, UPIII

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody (AB231576)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody (AB231576)

Formalin-fixed, paraffin-embedded rat kidney tissue stained for Uroplakin III using ab231576 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Uroplakin III antibody (AB231576)

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Western blot - Anti-Uroplakin III antibody (ab231576) at 2 µg/mL

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Mouse bladder lysate

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-Uroplakin III antibody (AB231576)

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Western blot - Anti-Uroplakin III antibody (ab231576) at 1 µg/mL

Lane 1:

Rat bladder lysate

Lane 2:

Mouse bladder lysate

Secondary

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HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution

Predicted band size: 31 kDa

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Supplier Data

Western blot - Anti-Uroplakin III antibody (AB231576)

All lanes:

Western blot - Anti-Uroplakin III antibody (ab231576) at 1 µg/mL

All lanes:

Human serum

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-Uroplakin III antibody (AB231576)

All lanes:

Western blot - Anti-Uroplakin III antibody (ab231576) at 1 µg/mL

All lanes:

Rat bladder lysate

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-Uroplakin III antibody (AB231576)

All lanes:

Western blot - Anti-Uroplakin III antibody (ab231576) at 2 µg/mL

All lanes:

Recombinant rat Uroplakin III protein

Predicted band size: 106 kDa,31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231576 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uroplakin III also known as UPK3 or UPIII is a transmembrane protein with a molecular weight of around 47 kDa. It is specifically expressed in the urothelium which lines the urinary tract including the bladder ureter and renal pelvis. Uroplakin III plays a structural role by contributing to the formation of the asymmetric unit membrane important for the stability and impermeability of the urothelial barrier.

Biological function summary

Uroplakin III takes part in the formation of the uroplakin complex which includes UPIa UPIb and UPII. It interacts with these proteins to form the urothelial plaques that are essential in maintaining the integrity of the urothelial surface. These plaques prevent urine from permeating back into the underlying tissues ensuring proper urinary function. Uroplakin III also participates in signal transduction mechanisms that may regulate cell proliferation within the urothelial lining.

Pathways

Uroplakin III works within the context of membrane assembly and bladder epithelium differentiation. It interacts with proteins like other uroplakins and cytoskeletal components to mediate the formation of apical urothelial plaques. The protein contributes to signaling pathways that involve cellular differentiation and proliferation ensuring the proper function and renewal of urothelial tissue.

Uroplakin III shows significant relevance in the context of bladder carcinoma and interstitial cystitis. Bladder carcinoma often exhibits altered expression of Uroplakin III suggesting its potential role as a diagnostic marker. In interstitial cystitis changes in Uroplakin III distribution might contribute to the compromised barrier function. Its altered interaction with proteins like uroplakin II can emphasize the link between these structural changes and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence (By similarity).

See full target information UPK3A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:25619 PubMed39463382

2024

Development of a novel immunocompetent murine tumor model for urothelial carcinoma using in vivo electroporation.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Soleder,Nicolas Gengenbacher,Carolin Mogler,Markus Eckstein,Anja Runge,Maximilian C Kriegmair,Hellmut G Augustin

JCI insight 9: PubMed38271096

2024

Purine nucleoside phosphorylase inhibition is an effective approach for the treatment of chemical hemorrhagic cystitis.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Wolf-Johnston,Youko Ikeda,Irina Zabbarova,Anthony J Kanai,Sheldon Bastacky,Robert Moldwin,Joel Nh Stern,Edwin K Jackson,Lori A Birder

Clinical and translational medicine 11:e482 PubMed34459123

2021

Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR-485-5p-mediated CXCL10 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Yingwei Chen,Xiaojie Chen,Haiyu Li,Yunpeng Li,Dong Cheng,Yi Tang,Haiqiang Sang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Uroplakin III antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody (AB231576)

Western blot - Anti-Uroplakin III antibody (AB231576)

Western blot - Anti-Uroplakin III antibody (AB231576)

Western blot - Anti-Uroplakin III antibody (AB231576)

Western blot - Anti-Uroplakin III antibody (AB231576)

Western blot - Anti-Uroplakin III antibody (AB231576)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise