AB187646

Anti-Uroplakin III antibody [EPR14420]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Uroplakin III antibody. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

UPK3, UPK3A, Uroplakin-3a, UP3a, Uroplakin III, UPIII

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody [EPR14420] (AB187646)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody [EPR14420] (AB187646)

Immunohistochemical analysis of paraffin-embedded Human urothelium carcinoma tissue labeling Uroplakin III with ab187646 at 1/32000 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Uroplakin III antibody [EPR14420] (AB187646)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Uroplakin III antibody [EPR14420] (AB187646)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedHeLa cells labeling Uroplakin III with ab187646 at 1/130 dilution compared to a Rabbit monoclonal IgG isotype control,followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

603 Alexa Fluor® 568

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)
665 Alexa Fluor® 647

Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14420

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/130", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/32000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uroplakin III also known as UPK3 or UPIII is a transmembrane protein with a molecular weight of around 47 kDa. It is specifically expressed in the urothelium which lines the urinary tract including the bladder ureter and renal pelvis. Uroplakin III plays a structural role by contributing to the formation of the asymmetric unit membrane important for the stability and impermeability of the urothelial barrier.

Biological function summary

Uroplakin III takes part in the formation of the uroplakin complex which includes UPIa UPIb and UPII. It interacts with these proteins to form the urothelial plaques that are essential in maintaining the integrity of the urothelial surface. These plaques prevent urine from permeating back into the underlying tissues ensuring proper urinary function. Uroplakin III also participates in signal transduction mechanisms that may regulate cell proliferation within the urothelial lining.

Pathways

Uroplakin III works within the context of membrane assembly and bladder epithelium differentiation. It interacts with proteins like other uroplakins and cytoskeletal components to mediate the formation of apical urothelial plaques. The protein contributes to signaling pathways that involve cellular differentiation and proliferation ensuring the proper function and renewal of urothelial tissue.

Uroplakin III shows significant relevance in the context of bladder carcinoma and interstitial cystitis. Bladder carcinoma often exhibits altered expression of Uroplakin III suggesting its potential role as a diagnostic marker. In interstitial cystitis changes in Uroplakin III distribution might contribute to the compromised barrier function. Its altered interaction with proteins like uroplakin II can emphasize the link between these structural changes and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence (By similarity).

See full target information UPK3A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 7:e10268 PubMed35600655

2022

A biomimetic hyaluronic acid-silk fibroin nanofiber scaffold promoting regeneration of transected urothelium.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqing Niu,Massimiliano Galluzzi,Fuming Deng,Zhang Zhao,Ming Fu,Liang Su,Weitang Sun,Wei Jia,Huimin Xia

Experimental & molecular medicine 50:36 PubMed29674622

2018

IL-33/ST2 axis mediates hyperplasia of intrarenal urothelium in obstructive renal injury.

Applications

Species

Mouse

Wei-Yu Chen,Jenq-Lin Yang,Yi-Hsiu Wu,Lung-Chih Li,Ru-Fang Li,Ya-Ting Chang,Lo-Hsin Dai,Wan-Chen Wang,Ya-Jen Chang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Uroplakin III antibody [EPR14420]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody [EPR14420] (AB187646)

Flow Cytometry (Intracellular) - Anti-Uroplakin III antibody [EPR14420] (AB187646)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise