Mouse Recombinant Monoclonal Uroplakin IIIB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse samples.
Images
![Western blot - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (AB255744), expandable thumbnail](https://content.abcam.com/products/images/uroplakin-iiib-antibody-c362-bsa-and-azide-free-ab255744--western-blot-img46326.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (AB255744)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (AB255744), expandable thumbnail](https://content.abcam.com/products/images/uroplakin-iiib-antibody-c362-bsa-and-azide-free-ab255744--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122505.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (AB255744)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (AB255744), expandable thumbnail](https://content.abcam.com/products/images/uroplakin-iiib-antibody-c362-bsa-and-azide-free-ab255744--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105098.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (AB255744)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Not recommended | Not recommended |
| Mouse | Tested | Tested |
| Rat | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Target data
Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence (By similarity).
Alternative names
Uroplakin-3b, UP3b, Uroplakin IIIb, p35, UPIIIb, Upk3b
Recommended products
Mouse Recombinant Monoclonal Uroplakin IIIB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse samples.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- C362
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255744 is the carrier-free version of Anti-Uroplakin IIIB antibody [C362] ab237778.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Uroplakin IIIB also known as UPK3B is a specific protein that is part of the family of uroplakins found in the urothelial plaques of bladder epithelium. It has a notable molecular mass of approximately 25 kDa. This protein is mainly expressed in the apical membrane of umbrella cells located in the bladder and other parts of the urinary tract. The expression pattern suggests a role in urothelial differentiation and barrier formation contributing to the impermeability and structural integrity of the urothelium.
- Biological function summary
Uroplakin IIIB is an integral part of the urothelial plaque complex. This complex consists of other proteins including Uroplakin Ia Ib and Uroplakin III which work collectively to stabilize the urothelial membrane. Uroplakin IIIB plays an important role in maintaining the barrier function that prevents urine from penetrating deeper layers of tissue thereby protecting underlying tissues from the toxic properties of urine. Its structural contributions are essential in preserving the function and health of the urinary bladder epithelium.
- Pathways
Uroplakin IIIB participates in pathways related to epithelial cell differentiation and tight junction formation essential for urothelial homeostasis. It interacts with other uroplakins and is linked to pathways that involve cell adhesion mechanisms. These pathways are important for the establishment of tight junctions necessary for the barrier function of urothelial cells. The coordination and expression of Uroplakin Ia Ib and III in these processes highlight their relationship with Uroplakin IIIB in ensuring cellular cohesion and impermeability.
- Associated diseases and disorders
Alterations in Uroplakin IIIB expression are associated with conditions such as bladder cancer and interstitial cystitis. Dysfunction in the uroplakin protein complex can lead to compromised bladder function and structural abnormalities. In bladder cancer specifically changes in Uroplakin IIIB along with its related proteins like Uroplakin III have been observed and studied as potential biomarkers for tumorigenesis. Understanding these connections provides insights into disease mechanisms and possible therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744), expandable thumbnail](https://content.abcam.com/products/images/uroplakin-iiib-antibody-c362-bsa-and-azide-free-ab255744--western-blot-img46326.jpg "Western blot - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)")
Western blot - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)
This data was developed using Anti-Uroplakin IIIB antibody [C362] ab237778, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: mouse lung (PMID: 12446744).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12446744).Exposure time: 26 seconds
All lanes: Western blot - Anti-Uroplakin IIIB antibody [C362] (Anti-Uroplakin IIIB antibody [C362] ab237778) at 1/1000 dilution
Lane 1: Mouse bladder tissue lysate at 20 µg
Lane 2: Mouse lung tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744), expandable thumbnail](https://content.abcam.com/products/images/uroplakin-iiib-antibody-c362-bsa-and-azide-free-ab255744--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122505.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)
This data was developed using Anti-Uroplakin IIIB antibody [C362] ab237778 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse bladder tissue labeling Uroplakin IIIB with Anti-Uroplakin IIIB antibody [C362] ab237778 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining in mouse urothelium.The section was incubated with Anti-Uroplakin IIIB antibody [C362] ab237778 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744), expandable thumbnail](https://content.abcam.com/products/images/uroplakin-iiib-antibody-c362-bsa-and-azide-free-ab255744--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105098.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)
This data was developed using Anti-Uroplakin IIIB antibody [C362] ab237778 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Uroplakin IIIB with Anti-Uroplakin IIIB antibody [C362] ab237778 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Negative control: No staining in mouse lung.The section was incubated with Anti-Uroplakin IIIB antibody [C362] ab237778 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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