Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (AB255744)

This data was developed using ab237778 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse bladder tissue labeling Uroplakin IIIB with ab237778 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining in mouse urothelium.The section was incubated with ab237778 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (AB255744)

This data was developed using ab237778 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Uroplakin IIIB with ab237778 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Negative control : No staining in mouse lung.The section was incubated with ab237778 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (AB255744)

This data was developed using ab237778, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : mouse lung (PMID : 12446744).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 12446744).

Exposure time : 26 seconds

All lanes:

Western blot - Anti-Uroplakin IIIB antibody [C362] (<a href='/en-us/products/primary-antibodies/uroplakin-iiib-antibody-c362-ab237778'>ab237778</a>) at 1/1000 dilution

Lane 1:

Mouse bladder tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 35 kDa

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