Anti-URP2/Kindlin-3 antibody [3D6]

• IHC-P

Ap

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-URP2/Kindlin-3 antibody [3D6] (AB173416)

IHC image of URP2/Kindlin-3 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab173416, 0.1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Lab

Western blot - Anti-URP2/Kindlin-3 antibody [3D6] (AB173416)

We believe that the band observed at approximately 33-kDa is a C-terminal cleavage fragment of URP2/Kindlin-3, which has been described in the literature (PMID 23012377). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-URP2/Kindlin-3 antibody [3D6] (ab173416) at 5 µg/mL

Lane 1:

Human spleen tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spleen-tissue-lysate-total-protein-ab29699'>ab29699</a>) at 20 µg

Lane 2:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 20 µg

Lane 3:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 20 µg

Lane 4:

THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 75 kDa

Observed band size: 33 kDa,76 kDa

true

Exposure time: 4min