Rabbit Polyclonal USE1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human USE1 aa 1-150.
View Alternative Names
USE1L, MDS032, USE1, Vesicle transport protein USE1, Putative MAPK-activating protein PM26, USE1-like protein, p31
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Use1 antibody (AB234867)
U-251 MG (human brain glioma cell line) cells stained for Use1 (green) using ab234867 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
- IP
Supplier Data
Immunoprecipitation - Anti-Use1 antibody (AB234867)
Use1 was immunoprecipitated from 0.5 mg PC-3 (human prostate adenocarcinoma cell line) whole cell lysate using ab234867 at 1/200 dilution.
Lane 1 : Control IgG IP in PC-3 whole cell lysate.
Lane 2 : ab234867 IP in PC-3 whole cell lysate.
Lane 3 : PC-3 whole cell lysate 10 μg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
All lanes:
Immunoprecipitation - Anti-Use1 antibody (ab234867)
Predicted band size: 29 kDa
false
- WB
Supplier Data
Western blot - Anti-Use1 antibody (AB234867)
All lanes:
Western blot - Anti-Use1 antibody (ab234867) at 1/500 dilution
Lane 1:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2:
Mouse heart lysate
Lane 3:
Mouse liver lysate
Lane 4:
Mouse brain lysate
Lane 5:
Mouse stomach lysate
Lane 6:
Mouse skeletal muscle lysate
Secondary
All lanes:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
false
Alternative Products
Complementary Products
![Flow Cytometry (Intracellular) - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)](https://content.abcam.com/products/images/wave-1-antibody-epr15021-n-terminal-ab185546--flow-cytometry-intracellular-img78282.jpg)
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Use1 plays an important role in the delivery and sorting of proteins within cells. It functions as a part of the SNARE complex which is important for the fusion of vesicles with target membranes. By ensuring the precise docking and release of vesicles this protein aids in maintaining proper cellular function. The SNARE complex including partners like Syntaxin 18 and BNIP1 ensures coordinated intracellular trafficking which is vital for cellular homeostasis.
Pathways
Use1 is deeply involved in the vesicular transport and cellular trafficking pathways. These pathways are essential for the movement of proteins and lipids between different organelles. Use1 works closely with the Golgi and endoplasmic reticulum trafficking pathways. It interacts with proteins such as Sec22b and Syntaxin 18 to facilitate vesicular transport processes. This activity ensures correct delivery of molecular cargos aiding in cellular function regulation and signal transduction.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Cellular and molecular biology (Noisy-le-Grand, France) 70:78-84 PubMed38836678
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com