Rabbit Polyclonal USE1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human USE1 aa 1-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human USE1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NZ43
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.
Alternative names
USE1L, MDS032, USE1, Vesicle transport protein USE1, Putative MAPK-activating protein PM26, USE1-like protein, p31
Recommended products
Rabbit Polyclonal USE1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human USE1 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human USE1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NZ43
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Use1 also known as UNS-1 or Protein Transport Protein Use1 is involved in the SNARE-mediated transport mechanism. It has a molecular mass of approximately 29 kDa. This protein is expressed in various tissues with significant presence in the endoplasmic reticulum and Golgi apparatus. Structurally Use1 interacts with other SNARE proteins to regulate vesicle fusion processes essential for intracellular transport.
- Biological function summary
Use1 plays an important role in the delivery and sorting of proteins within cells. It functions as a part of the SNARE complex which is important for the fusion of vesicles with target membranes. By ensuring the precise docking and release of vesicles this protein aids in maintaining proper cellular function. The SNARE complex including partners like Syntaxin 18 and BNIP1 ensures coordinated intracellular trafficking which is vital for cellular homeostasis.
- Pathways
Use1 is deeply involved in the vesicular transport and cellular trafficking pathways. These pathways are essential for the movement of proteins and lipids between different organelles. Use1 works closely with the Golgi and endoplasmic reticulum trafficking pathways. It interacts with proteins such as Sec22b and Syntaxin 18 to facilitate vesicular transport processes. This activity ensures correct delivery of molecular cargos aiding in cellular function regulation and signal transduction.
- Associated diseases and disorders
Mutations or dysfunction of Use1 have been associated with neurodegenerative diseases and certain metabolic disorders. Neurodegenerative conditions like Alzheimer's disease show disrupted protein transport implicating Use1's role in proper neuronal function. Additionally Use1 connects with proteins like Syntaxin 5 highlighting its involvement in metabolic pathways that when altered may lead to disorders. Research continues to investigate Use1 as a potential therapeutic target for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Use1 antibody (ab234867)
All lanes: Western blot - Anti-Use1 antibody (ab234867) at 1/500 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2: Mouse heart lysate
Lane 3: Mouse liver lysate
Lane 4: Mouse brain lysate
Lane 5: Mouse stomach lysate
Lane 6: Mouse skeletal muscle lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Use1 antibody (ab234867)
U-251 MG (human brain glioma cell line) cells stained for Use1 (green) using ab234867 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Immunoprecipitation - Anti-Use1 antibody (ab234867)
Use1 was immunoprecipitated from 0.5 mg PC-3 (human prostate adenocarcinoma cell line) whole cell lysate using ab234867 at 1/200 dilution.
Lane 1: Control IgG IP in PC-3 whole cell lysate.
Lane 2: ab234867 IP in PC-3 whole cell lysate.
Lane 3: PC-3 whole cell lysate 10 μg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
All lanes: Immunoprecipitation - Anti-Use1 antibody (ab234867)
Predicted band size: 29 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com