Mouse Polyclonal USF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human USF1.
Images
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human USF1. Database link P22415
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Alternative names
BHLHB11, USF, USF1, Upstream stimulatory factor 1, Class B basic helix-loop-helix protein 11, Major late transcription factor 1, bHLHb11
Recommended products
Mouse Polyclonal USF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human USF1.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human USF1. Database link P22415
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USF1 also known as Upstream Stimulatory Factor 1 is a transcription factor with a molecular weight of about 43 kDa. It binds to specific DNA sequences to regulate transcription of target genes. This protein expresses in many tissues and is particularly abundant in the liver adipose tissue and muscle. It belongs to the bHLH (basic helix-loop-helix) family of transcription factors and it dimerizes to function effectively.
- Biological function summary
USF1 plays a role in controlling lipid and glucose metabolism. As part of a transcriptional complex it regulates the expression of genes involved in metabolic processes. USF1 influences the transcription of genes tied to cholesterol biosynthesis such as HMGCR (3-hydroxy-3-methylglutaryl-CoA reductase) and genes connected to fatty acid metabolism. Its activity helps manage the body's metabolic balance by controlling enzyme levels in these pathways.
- Pathways
USF1 integrates within the lipid metabolism pathway and the insulin signaling pathway. In lipid metabolism it regulates enzymes that are critical for lipid homeostasis such as those involved in cholesterol synthesis. In the insulin signaling pathway USF1 interacts with proteins like SREBP-1c (Sterol Regulatory Element-Binding Protein 1c) affecting the expression of glucoregulatory genes. These interactions coordinate responses to metabolic demands and help maintain energy balance within cells.
- Associated diseases and disorders
Several studies associate USF1 with dyslipidemia and metabolic syndrome. Variants of USF1 link to alterations in lipid profiles potentially increasing the risk for cardiovascular diseases. The protein interacts with other key regulators such as LXR (Liver X Receptor) influencing cholesterol metabolism and contributing to atherosclerosis development. Understanding USF1's role in these conditions can help develop targeted therapies for metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-USF1 antibody (ab167693)
All lanes: Western blot - Anti-USF1 antibody (ab167693) at 1 µg/mL
Lane 1: USF1 transfected 293T cell lysate at 15 µL
Lane 2: Non-transfected 293T cell lysate at 15 µL
Predicted band size: 34 kDa
Immunocytochemistry/ Immunofluorescence - Anti-USF1 antibody (ab167693)
Immunofluorescent analysis of HeLa cells labeling USF1 with ab167693 at 10 µg/ml.
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