AB180717

Anti-USF1 antibody

Name: Anti-USF1 antibody (ab180717) | Abcam
Brand: Abcam Limited
SKU: AB180717
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal USF1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human USF1.

View Alternative Names

BHLHB11, USF, USF1, Upstream stimulatory factor 1, Class B basic helix-loop-helix protein 11, Major late transcription factor 1, bHLHb11

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-USF1 antibody (AB180717)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-USF1 antibody (AB180717)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180717. Blue DAPI for nuclear staining.

Supplier Data

Western blot - Anti-USF1 antibody (AB180717)

All lanes:

Western blot - Anti-USF1 antibody (ab180717) at 1/500 dilution

All lanes:

K562 cell line extract

Predicted band size: 28 kDa,31 kDa,34 kDa,46 kDa,70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human USF1.

P22415

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/500", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USF1 also known as Upstream Stimulatory Factor 1 is a transcription factor with a molecular weight of about 43 kDa. It binds to specific DNA sequences to regulate transcription of target genes. This protein expresses in many tissues and is particularly abundant in the liver adipose tissue and muscle. It belongs to the bHLH (basic helix-loop-helix) family of transcription factors and it dimerizes to function effectively.

Biological function summary

USF1 plays a role in controlling lipid and glucose metabolism. As part of a transcriptional complex it regulates the expression of genes involved in metabolic processes. USF1 influences the transcription of genes tied to cholesterol biosynthesis such as HMGCR (3-hydroxy-3-methylglutaryl-CoA reductase) and genes connected to fatty acid metabolism. Its activity helps manage the body's metabolic balance by controlling enzyme levels in these pathways.

Pathways

USF1 integrates within the lipid metabolism pathway and the insulin signaling pathway. In lipid metabolism it regulates enzymes that are critical for lipid homeostasis such as those involved in cholesterol synthesis. In the insulin signaling pathway USF1 interacts with proteins like SREBP-1c (Sterol Regulatory Element-Binding Protein 1c) affecting the expression of glucoregulatory genes. These interactions coordinate responses to metabolic demands and help maintain energy balance within cells.

Several studies associate USF1 with dyslipidemia and metabolic syndrome. Variants of USF1 link to alterations in lipid profiles potentially increasing the risk for cardiovascular diseases. The protein interacts with other key regulators such as LXR (Liver X Receptor) influencing cholesterol metabolism and contributing to atherosclerosis development. Understanding USF1's role in these conditions can help develop targeted therapies for metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

See full target information USF1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 58:e13755 PubMed39329203

2024

Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiexiang Zhao,Kang Tang,Gurong Jiang,Xinyan Yang,Manman Cui,Cong Wan,Zhaoxiang Ouyang,Yi Zheng,Zhaoting Liu,Mei Wang,Xiao-Yang Zhao,Gang Chang

Frontiers in molecular biosciences 9:758968 PubMed35155573

2022

USF1 Transcriptionally Regulates UGT1A3 and Promotes Lung Adenocarcinoma Progression by Regulating Neurotrophin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Yun-Xia Zhao,Xiang-Wei Zhang,Yuan-Zhu Jiang,Wei Ma,Lin Zhang,Wei Dong

International journal of molecular sciences 22: PubMed33947045

2021

Transcriptional Suppression of Diabetic Nephropathy with Novel Gene Silencer Pyrrole-Imidazole Polyamides Preventing USF1 Binding to the TGF-β1 Promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Makiyo Okamura,Noboru Fukuda,Shu Horikoshi,Hiroki Kobayashi,Akiko Tsunemi,Yurie Akiya,Morito Endo,Taro Matsumoto,Masanori Abe

Experimental and therapeutic medicine 16:4415-4422 PubMed30542391

2018

Upstream transcription factor 1 prompts malignancies of cervical cancer primarily by transcriptionally activating p65 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Wang,Shujuan Yao,Hongjing Jiang,Jing Dong,Xiujuan Cui,Xiangyu Tian,Yanyan Guo,Shiqian Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USF1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-USF1 antibody (AB180717)

Western blot - Anti-USF1 antibody (AB180717)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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