Anti-USF1 Antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USF1 Antibody (AB327226)

Immunohistochemical staining of human tonsil using ab327226 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-USF1 Antibody (AB327226)

Immunofluorescent staining of human cell line MCF7 using ab327226 at 0.25–2 µg/ml, showing localization to nucleoplasm.

• ChIP

Supplier Data

ChIP - Anti-USF1 Antibody (AB327226)

ChIP-Exo-Seq composite graph for ab327226 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-USF1 Antibody (AB327226)

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using ab327226 at 0.04–0.4 µg/ml. Remaining relative intensity is presented.

All lanes:

Western blot - Anti-USF1 Antibody (ab327226)

Lane 1:

Marker

Lane 2:

U2OS cells transfected with target specific siRNA probe #1

Lane 3:

U2OS cells transfected with target specific siRNA probe #2

Lane 4:

U2OS cells transfected with control siRNA

Predicted band size: 33.54 kDa

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