AB125020

Anti-USF1 antibody [EPR6430]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal USF1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

View Alternative Names

BHLHB11, USF, USF1, Upstream stimulatory factor 1, Class B basic helix-loop-helix protein 11, Major late transcription factor 1, bHLHb11

3 Images

Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (AB125020)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (AB125020)

Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab125020 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

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Western blot - Anti-USF1 antibody [EPR6430] (AB125020)

All lanes:

Western blot - Anti-USF1 antibody [EPR6430] (ab125020) at 1/10000 dilution

Lane 1:

K-562 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Hela cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

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OI-RD Scanning - Anti-USF1 antibody [EPR6430] (AB125020)

OI-RD Scanning

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OI-RD Scanning - Anti-USF1 antibody [EPR6430] (AB125020)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-USF1 antibody [EPR6430] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6430

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USF1 also known as Upstream Stimulatory Factor 1 is a transcription factor with a molecular weight of about 43 kDa. It binds to specific DNA sequences to regulate transcription of target genes. This protein expresses in many tissues and is particularly abundant in the liver adipose tissue and muscle. It belongs to the bHLH (basic helix-loop-helix) family of transcription factors and it dimerizes to function effectively.

Biological function summary

USF1 plays a role in controlling lipid and glucose metabolism. As part of a transcriptional complex it regulates the expression of genes involved in metabolic processes. USF1 influences the transcription of genes tied to cholesterol biosynthesis such as HMGCR (3-hydroxy-3-methylglutaryl-CoA reductase) and genes connected to fatty acid metabolism. Its activity helps manage the body's metabolic balance by controlling enzyme levels in these pathways.

Pathways

USF1 integrates within the lipid metabolism pathway and the insulin signaling pathway. In lipid metabolism it regulates enzymes that are critical for lipid homeostasis such as those involved in cholesterol synthesis. In the insulin signaling pathway USF1 interacts with proteins like SREBP-1c (Sterol Regulatory Element-Binding Protein 1c) affecting the expression of glucoregulatory genes. These interactions coordinate responses to metabolic demands and help maintain energy balance within cells.

Several studies associate USF1 with dyslipidemia and metabolic syndrome. Variants of USF1 link to alterations in lipid profiles potentially increasing the risk for cardiovascular diseases. The protein interacts with other key regulators such as LXR (Liver X Receptor) influencing cholesterol metabolism and contributing to atherosclerosis development. Understanding USF1's role in these conditions can help develop targeted therapies for metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

See full target information USF1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e2204711 PubMed36307901

2022

Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Yun Peng,Dian-Kui Cai,Ren-Li Zeng,Chao-Yang Zhang,Guan-Cheng Li,Si-Fan Chen,Xiao-Qing Yuan,Li Peng

OncoTargets and therapy 14:3687-3700 PubMed34140779

2021

miR-210-3p Promotes Lung Cancer Development and Progression by Modulating USF1 and PCGF3.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Hongyu Zhang,Jianyin Zhang,Le Shen,Jing Yang,Yan Wang,JinXiu Ma,Bing Zhuan

Journal of advanced research 26:123-135 PubMed33133688

2020

Inhibition of long noncoding RNA HIF1A-AS2 confers protection against atherosclerosis ATF2 downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Li,Junhui Xing,Jielei Zhang,Jianwu Jiang,Xuemeng Liu,Di Zhao,Yanzhou Zhang

Cell proliferation 52:e12687 PubMed31468594

2019

LncRNA LOXL1-AS1 facilitates the tumorigenesis and stemness of gastric carcinoma via regulation of miR-708-5p/USF1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Sun,Jian Li,Fan Li,Huanqin Li,Songhua Bei,Xiaohong Zhang,Li Feng

FEBS letters 592:2725-2738 PubMed30054905

2018

Upstream stimulating factor 1 suppresses autophagy and hepatic lipid droplet catabolism by activating mTOR.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Guo,Weiwei Fang,Xiehui Chen,Yajun Lin,Gang Hu,Jie Wei,Xiaoyi Zhang,Chunxiao Yang,Jian Li

Experimental and therapeutic medicine 16:3518-3524 PubMed30233704

2018

USF1 promotes the development of knee osteoarthritis by activating the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiandong Song,Min Zhu,Hao Li,Bo Liu,Zhaowei Yan,Weican Wang,Hongyi Li,Jiping Sun,Shixing Li

Carcinogenesis 34:397-405 PubMed23161610

2012

Src kinase is a direct target of apigenin against UVB-induced skin inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Sanguine Byun,Jiman Park,Eunjung Lee,Semi Lim,Jae Gak Yu,Seung Joon Lee,Hanyong Chen,Zigang Dong,Ki Won Lee,Hyong Joo Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USF1 antibody [EPR6430]

Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (AB125020)

Western blot - Anti-USF1 antibody [EPR6430] (AB125020)

OI-RD Scanning - Anti-USF1 antibody [EPR6430] (AB125020)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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