Rabbit Recombinant Monoclonal USF1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.
Images
![Western blot - Anti-USF1 antibody [EPR6430] (AB125020), expandable thumbnail](https://content.abcam.com/products/images/usf1-antibody-epr6430-ab125020--western-blot-img106688.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-USF1 antibody [EPR6430] (AB125020)")
![Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (AB125020), expandable thumbnail](https://content.abcam.com/products/images/usf1-antibody-epr6430-ab125020--flow-cytometry-intracellular-img27412.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (AB125020)")
![OI-RD Scanning - Anti-USF1 antibody [EPR6430] (AB125020), expandable thumbnail](https://content.abcam.com/products/images/usf1-antibody-epr6430-ab125020--oi-rd-scanning-img95196.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-USF1 antibody [EPR6430] (AB125020)")
Publications
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- Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e22047112022Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiang-Yun Peng,Dian-Kui Cai,Ren-Li Zeng,Chao-Yang Zhang,Guan-Cheng Li,Si-Fan Chen,Xiao-Qing Yuan,Li PengPubMed 36307901
- OncoTargets and therapy 14:3687-37002021miR-210-3p Promotes Lung Cancer Development and Progression by Modulating USF1 and PCGF3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Chen,Hongyu Zhang,Jianyin Zhang,Le Shen,Jing Yang,Yan Wang,JinXiu Ma,Bing ZhuanPubMed 34140779
- Journal of advanced research 26:123-1352020Inhibition of long noncoding RNA HIF1A-AS2 confers protection against atherosclerosis ATF2 downregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengcheng Li,Junhui Xing,Jielei Zhang,Jianwu Jiang,Xuemeng Liu,Di Zhao,Yanzhou ZhangPubMed 33133688
- Cell proliferation 52:e126872019LncRNA LOXL1-AS1 facilitates the tumorigenesis and stemness of gastric carcinoma via regulation of miR-708-5p/USF1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Sun et. al.PubMed 31468594
- FEBS letters 592:2725-27382018Upstream stimulating factor 1 suppresses autophagy and hepatic lipid droplet catabolism by activating mTOR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Guo et. al.PubMed 30054905
- Experimental and therapeutic medicine 16:3518-35242018USF1 promotes the development of knee osteoarthritis by activating the NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiandong Song et. al.PubMed 30233704
- Carcinogenesis 34:397-4052012Src kinase is a direct target of apigenin against UVB-induced skin inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanguine Byun,Jiman Park,Eunjung Lee,Semi Lim,Jae Gak Yu,Seung Joon Lee,Hanyong Chen,Zigang Dong,Ki Won Lee,Hyong Joo LeePubMed 23161610
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Alternative names
BHLHB11, USF, USF1, Upstream stimulatory factor 1, Class B basic helix-loop-helix protein 11, Major late transcription factor 1, bHLHb11
Recommended products
Rabbit Recombinant Monoclonal USF1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR6430
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 9.5 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USF1 also known as Upstream Stimulatory Factor 1 is a transcription factor with a molecular weight of about 43 kDa. It binds to specific DNA sequences to regulate transcription of target genes. This protein expresses in many tissues and is particularly abundant in the liver adipose tissue and muscle. It belongs to the bHLH (basic helix-loop-helix) family of transcription factors and it dimerizes to function effectively.
- Biological function summary
USF1 plays a role in controlling lipid and glucose metabolism. As part of a transcriptional complex it regulates the expression of genes involved in metabolic processes. USF1 influences the transcription of genes tied to cholesterol biosynthesis such as HMGCR (3-hydroxy-3-methylglutaryl-CoA reductase) and genes connected to fatty acid metabolism. Its activity helps manage the body's metabolic balance by controlling enzyme levels in these pathways.
- Pathways
USF1 integrates within the lipid metabolism pathway and the insulin signaling pathway. In lipid metabolism it regulates enzymes that are critical for lipid homeostasis such as those involved in cholesterol synthesis. In the insulin signaling pathway USF1 interacts with proteins like SREBP-1c (Sterol Regulatory Element-Binding Protein 1c) affecting the expression of glucoregulatory genes. These interactions coordinate responses to metabolic demands and help maintain energy balance within cells.
- Associated diseases and disorders
Several studies associate USF1 with dyslipidemia and metabolic syndrome. Variants of USF1 link to alterations in lipid profiles potentially increasing the risk for cardiovascular diseases. The protein interacts with other key regulators such as LXR (Liver X Receptor) influencing cholesterol metabolism and contributing to atherosclerosis development. Understanding USF1's role in these conditions can help develop targeted therapies for metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-USF1 antibody [EPR6430] (ab125020), expandable thumbnail](https://content.abcam.com/products/images/usf1-antibody-epr6430-ab125020--western-blot-img106688.jpg "Western blot - Anti-USF1 antibody [EPR6430] (ab125020)")
Western blot - Anti-USF1 antibody [EPR6430] (ab125020)
All lanes: Western blot - Anti-USF1 antibody [EPR6430] (ab125020) at 1/10000 dilution
Lane 1: K-562 cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: Hela cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
![Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (ab125020), expandable thumbnail](https://content.abcam.com/products/images/usf1-antibody-epr6430-ab125020--flow-cytometry-intracellular-img27412.jpg "Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (ab125020)")
Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (ab125020)
Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab125020 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
![OI-RD Scanning - Anti-USF1 antibody [EPR6430] (ab125020), expandable thumbnail](https://content.abcam.com/products/images/usf1-antibody-epr6430-ab125020--oi-rd-scanning-img95196.jpg "OI-RD Scanning - Anti-USF1 antibody [EPR6430] (ab125020)")
OI-RD Scanning - Anti-USF1 antibody [EPR6430] (ab125020)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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