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AB240016

Anti-USF1 antibody [EPR6430] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal USF1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BHLHB11, USF, USF1, Upstream stimulatory factor 1, Class B basic helix-loop-helix protein 11, Major late transcription factor 1, bHLHb11

2 Images
Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] - BSA and Azide free (AB240016)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] - BSA and Azide free (AB240016)

Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab125020 at 1/10 dilution (red) or a rabbit IgG (negative) (green). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125020).

OI-RD Scanning - Anti-USF1 antibody [EPR6430] - BSA and Azide free (AB240016)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-USF1 antibody [EPR6430] - BSA and Azide free (AB240016)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6430

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab240016 is the carrier-free version of ab125020.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USF1 also known as Upstream Stimulatory Factor 1 is a transcription factor with a molecular weight of about 43 kDa. It binds to specific DNA sequences to regulate transcription of target genes. This protein expresses in many tissues and is particularly abundant in the liver adipose tissue and muscle. It belongs to the bHLH (basic helix-loop-helix) family of transcription factors and it dimerizes to function effectively.
Biological function summary

USF1 plays a role in controlling lipid and glucose metabolism. As part of a transcriptional complex it regulates the expression of genes involved in metabolic processes. USF1 influences the transcription of genes tied to cholesterol biosynthesis such as HMGCR (3-hydroxy-3-methylglutaryl-CoA reductase) and genes connected to fatty acid metabolism. Its activity helps manage the body's metabolic balance by controlling enzyme levels in these pathways.

Pathways

USF1 integrates within the lipid metabolism pathway and the insulin signaling pathway. In lipid metabolism it regulates enzymes that are critical for lipid homeostasis such as those involved in cholesterol synthesis. In the insulin signaling pathway USF1 interacts with proteins like SREBP-1c (Sterol Regulatory Element-Binding Protein 1c) affecting the expression of glucoregulatory genes. These interactions coordinate responses to metabolic demands and help maintain energy balance within cells.

Several studies associate USF1 with dyslipidemia and metabolic syndrome. Variants of USF1 link to alterations in lipid profiles potentially increasing the risk for cardiovascular diseases. The protein interacts with other key regulators such as LXR (Liver X Receptor) influencing cholesterol metabolism and contributing to atherosclerosis development. Understanding USF1's role in these conditions can help develop targeted therapies for metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
See full target information USF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 28: PubMed36677863

2023

Sinomenine Hydrochloride Can Ameliorate Benign Prostatic Hyperplasia by Lowering the 5α-Reductase 2 Level and Regulating the Balance between the Proliferation and Apoptosis of Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mao-Si Fan,Yue-Fei Xia,Rui-Han Ye,Ze-Rui Sun,Ming-Yue Wang,Meng-Fei An,Shao-Shi Zhang,Li-Juan Zhang,Yun-Li Zhao,Ze-Min Xiang,Jun Sheng
View all publications
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Product promise

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