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AB244404

Anti-USH1C/Harmonin antibody

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Rabbit Polyclonal USH1C/Harmonin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human USH1C aa 350-450.

View Alternative Names

AIE75, USH1C, Harmonin, Antigen NY-CO-38/NY-CO-37, Autoimmune enteropathy-related antigen AIE-75, Protein PDZ-73, Renal carcinoma antigen NY-REN-3, Usher syndrome type-1C protein

4 Images
Western blot - Anti-USH1C/Harmonin antibody (AB244404)
  • WB

Unknown

Western blot - Anti-USH1C/Harmonin antibody (AB244404)

All lanes:

Western blot - Anti-USH1C/Harmonin antibody (ab244404) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

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NBT-II (Rat cell line) cell lysate

Predicted band size: 62 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USH1C/Harmonin antibody (AB244404)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USH1C/Harmonin antibody (AB244404)

Paraffin-embedded human duodenum tissue stained for USH1C/Harmonin using ab244404 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-USH1C/Harmonin antibody (AB244404)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-USH1C/Harmonin antibody (AB244404)

PFA-fixed, Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells stained for USH1C/Harmonin (green) using ab244404 at 4 μg/ml in ICC/IF.

Western blot - Anti-USH1C/Harmonin antibody (AB244404)
  • WB

Unknown

Western blot - Anti-USH1C/Harmonin antibody (AB244404)

All lanes:

Western blot - Anti-USH1C/Harmonin antibody (ab244404) at 0.4 µg/mL

Lane 1:

Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

Lane 2:

PC-3 (Human prostate adenocarcinoma cell line) cell lysate

Predicted band size: 62 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human USH1C aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6N9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The USH1C also known as Harmonin or Harmoneem is a protein with a molecular weight of approximately 73 kDa. It plays a mechanical role in connecting the mechanosensory hair bundle of the inner ear and the junctional complex of the retina. Harmonin protein expression mainly occurs in sensory hair cells of the inner ear and photoreceptor cells of the retina. These areas show its critical involvement in maintaining function in the sensory systems.
Biological function summary

The Harmonin protein participates in establishing and maintaining the structure and function of the hair cells and photoreceptors. It is a part of the multi-protein complex known as the Usher protein complex which includes other proteins such as cadherin and myosin. This complex ensures proper cell signaling and structural support necessary for sensory perception related to hearing and vision.

Pathways

The Harmonin protein is involved in the mechanotransduction pathway and the visual phototransduction pathway. It plays a role in converting mechanical signals into electrical ones within hair cells and is similarly vital in photoreceptor cells. Through these pathways Harmonin interacts with proteins like protocadherin and myosin VIIa highlighting its function in signal transduction processes within sensory cells.

Harmonin mutations link to Usher syndrome type 1C a disorder characterized by sensorineural hearing loss and retinitis pigmentosa. Defective Harmonin interactions disturb the Usher protein complex leading to the symptoms of this syndrome. Proteins such as myosin VIIa also relate to Usher syndrome as they function alongside Harmonin in maintaining sensory cell integrity. Understanding these connections offers insights into disease mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal development and maintenance of cochlear hair cell bundles (By similarity). As part of the intermicrovillar adhesion complex/IMAC plays a role in brush border differentiation, controlling microvilli organization and length. Probably plays a central regulatory role in the assembly of the complex, recruiting CDHR2, CDHR5 and MYO7B to the microvilli tips (PubMed : 24725409, PubMed : 26812018).
See full target information USH1C
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