AB150820

Anti-USH1G antibody

Name: Anti-USH1G antibody (ab150820) | Abcam
Brand: Abcam Limited
SKU: AB150820
Price: 630 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal USH1G antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USH1G aa 50-200.

View Alternative Names

SANS, USH1G, pre-mRNA splicing regulator USH1G, Scaffold protein containing ankyrin repeats and SAM domain, Usher syndrome type-1G protein

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USH1G antibody (AB150820)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USH1G antibody (AB150820)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue tissue, labelling USH1G with ab150820 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-USH1G antibody (AB150820)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-USH1G antibody (AB150820)

Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles. Recommended concentration of ab150820 1-4 µg/ml. Cells treated with PFA/Triton X-100.

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Western blot - Anti-USH1G antibody (AB150820)

Lane 1 : NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)

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Western blot - Anti-USH1G antibody (ab150820)

Predicted band size: 50 kDa,51 kDa

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Western blot - Anti-USH1G antibody (AB150820)

All lanes:

Western blot - Anti-USH1G antibody (ab150820) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human USH1G aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q495M9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "Recommend PFA Fixation and Triton X-100 treatment" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USH1G also known as SANS (Scaffold protein containing Ankyrin Repeats and SAM domain) plays a mechanical role in cellular structures and processes. This protein has a molecular mass of approximately 54 kDa. It is expressed in the inner ear retina and possibly other tissues suggesting a broad role in sensory functions. USH1G is involved in maintaining protein interactions important for cellular architecture and function contributing to the organization of the cytoskeleton.

Biological function summary

USH1G acts as a scaffold protein that assists in forming protein complexes vital for mechanosensory hair cells in the ear. It participates in linking proteins such as harmonin and the myosin motor proteins forming part of a ternary complex that is essential within the stereocilia of hair cells. This complex ensures proper hair cell mechanoelectrical transduction which is necessary for sound and balance perception.

Pathways

USH1G operates within key mechanotransduction pathways of the sensory cells. It interacts significantly with the PDZD7 and harmonin proteins providing critical support for these pathways. The PDZD7 protein in particular is also a part of the Usher protein interactome which is essential for developing and maintaining hair cell function in the auditory system.

Malfunctions or mutations in the USH1G gene associate with Usher syndrome type 1G a condition leading to hearing loss and visual impairments. The protein also relates to retinitis pigmentosa through its role in the Usher protein complex which involves other proteins like cadherin-23. Disruptions in the interactions among these proteins often result in sensory deficits characteristic of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays a role in pre-mRNA splicing by regulating the release and transfer of U4/U6.U5 tri-small nuclear ribonucleoprotein (tri-snRNP) complexes from their assembly site in Cajal bodies to nuclear speckles, thereby contributing to the assembly of the pre-catalytic spliceosome on target pre-mRNAs (PubMed : 34023904). May also participate in recycling of snRNPs back to Cajal bodies during splicing (PubMed : 34023904). Plays a role in regulating MAGI2-mediated endocytosis (PubMed : 24608321). Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal development and maintenance of cochlear hair cell bundles. Required for normal hearing.

See full target information USH1G

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed38194286

2024

Extended time frame for restoring inner ear function through gene therapy in Usher1G preclinical model.

Applications

Unspecified application

Species

Unspecified reactive species

Ghizlene Lahlou,Charlotte Calvet,François Simon,Vincent Michel,Lauranne Alciato,Baptiste Plion,Jacques Boutet de Monvel,Marie-José Lecomte,Mathieu Beraneck,Christine Petit,Saaid Safieddine

Current biology : CB 32:2480-2493.e6 PubMed35537456

2022

The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Trupinić,Barbara Kokanović,Ivana Ponjavić,Ivan Barišić,Siniša Šegvić,Arian Ivec,Iva M Tolić

International journal of molecular sciences 23: PubMed35163494

2022

Usher Syndrome Belongs to the Genetic Diseases Associated with Radiosensitivity: Influence of the ATM Protein Kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Joëlle Al-Choboq,Mélanie L Ferlazzo,Laurène Sonzogni,Adeline Granzotto,Laura El-Nachef,Mira Maalouf,Elise Berthel,Nicolas Foray

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-USH1G antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USH1G antibody (AB150820)

Immunocytochemistry/ Immunofluorescence - Anti-USH1G antibody (AB150820)

Western blot - Anti-USH1G antibody (AB150820)

Western blot - Anti-USH1G antibody (AB150820)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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