Rabbit Polyclonal USO1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USO1 aa 1-200.
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Publications
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- Journal of proteomics 229:1039262020Identification of p115 as a novel ACSL4 interacting protein and its role in regulating ACSL4 degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesProgga Sen,Chin Fung Kelvin Kan,Amar B Singh,Monica Rius,Fredric B Kraemer,Elizabeth Sztul,Jingwen LiuPubMed 32736139
- Frontiers in bioengineering and biotechnology 8:4562020Optimisation of a Novel Bio-Substrate as a Treatment for Atrophic Age-Related Macular Degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRachel McCormick et. al.PubMed 32500067
- Oncogene 37:1308-13252017A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBreege V Howley,Laura A Link,Simon Grelet,Maya El-Sabban,Philip H HowePubMed 29249802
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human USO1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O60763
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/20000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Alternative names
VDP, USO1, General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, Vesicle-docking protein, TAP
Recommended products
Rabbit Polyclonal USO1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USO1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human USO1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O60763
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USO1 also known as p115 is a heavy protein weighing approximately 112 kDa. It functions as a general vesicle tethering factor critical for the fusion of Golgi-derived vesicles. USO1 operates by binding to both vesicle and target membranes facilitating vesicular trafficking within the Golgi apparatus. It expresses broadly across various cell types underpinning its essential role in intracellular transport processes.
- Biological function summary
The USO1 protein plays an instrumental role in maintaining Golgi structure and function. It belongs to the transport protein particle (TRAPP) complex and works closely with several other proteins in the vesicle tethering and fusion steps. By interacting with the Golgi SNAREs USO1 helps mediate the fusion process necessary for protein and lipid transport.
- Pathways
USO1 integrates into cellular trafficking and transport pathways. It participates in the Golgi transport pathway working alongside proteins like GM130 and NSF to ensure stabilized vesicle docking and fusion. USO1's function connects it to the secretory pathway where it assists in uniting the endoplasmic reticulum's production line with the Golgi's packaging and shipping center.
- Associated diseases and disorders
Abnormalities in USO1 function associate with neurological disorders and cancer progressions. USO1's interaction with GM130 links its dysfunctional activity to neuronal migration defects. Additionally aberrations in USO1 levels can influence cancer cell proliferation denoting its potential as a target for therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-USO1 antibody (ab102470)
7.5% SDS-PAGE.
All lanes: Western blot - Anti-USO1 antibody (ab102470) at 1/10000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 100 kDa, 102 kDa, 108 kDa, 119 kDa, 12 kDa, 67 kDa, 79 kDa, 97 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USO1 antibody (ab102470)
ab102470 at 1/500 dilution staining USO1 in Human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
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