Rabbit Polyclonal USO1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USO1 aa 700 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
VDP, VDP, USO1, General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, Vesicle-docking protein, TAP
Rabbit Polyclonal USO1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USO1 aa 700 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
USO1 also known as p115 is a heavy protein weighing approximately 112 kDa. It functions as a general vesicle tethering factor critical for the fusion of Golgi-derived vesicles. USO1 operates by binding to both vesicle and target membranes facilitating vesicular trafficking within the Golgi apparatus. It expresses broadly across various cell types underpinning its essential role in intracellular transport processes.
The USO1 protein plays an instrumental role in maintaining Golgi structure and function. It belongs to the transport protein particle (TRAPP) complex and works closely with several other proteins in the vesicle tethering and fusion steps. By interacting with the Golgi SNAREs USO1 helps mediate the fusion process necessary for protein and lipid transport.
USO1 integrates into cellular trafficking and transport pathways. It participates in the Golgi transport pathway working alongside proteins like GM130 and NSF to ensure stabilized vesicle docking and fusion. USO1's function connects it to the secretory pathway where it assists in uniting the endoplasmic reticulum's production line with the Golgi's packaging and shipping center.
Abnormalities in USO1 function associate with neurological disorders and cancer progressions. USO1's interaction with GM130 links its dysfunctional activity to neuronal migration defects. Additionally aberrations in USO1 levels can influence cancer cell proliferation denoting its potential as a target for therapeutic strategies.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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7.5% SDS PAGE
All lanes: Western blot - Anti-USO1 antibody (ab184014) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 108 kDa
Immunofluorescence analysis of methanol fixed A431 (human epidermoid carcinoma cell line) cells labelling USO1 in the Golgi apparatus with ab184014 at 1/500 dilution (green) and counterstained with Hoechst 33342. Cells were fixed in 100% methanol for 5 mins.
5% SDS-PAGE
All lanes: Western blot - Anti-USO1 antibody (ab184014) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Lane 2: C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
Predicted band size: 108 kDa
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