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Rabbit Polyclonal USO1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USO1 aa 700 to C-terminus.

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Images

Western blot - Anti-USO1 antibody (AB184014), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-USO1 antibody (AB184014), expandable thumbnail
  • Western blot - Anti-USO1 antibody (AB184014), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human USO1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60763

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.

Alternative names

Recommended products

Rabbit Polyclonal USO1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USO1 aa 700 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human USO1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60763
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

USO1 also known as p115 is a heavy protein weighing approximately 112 kDa. It functions as a general vesicle tethering factor critical for the fusion of Golgi-derived vesicles. USO1 operates by binding to both vesicle and target membranes facilitating vesicular trafficking within the Golgi apparatus. It expresses broadly across various cell types underpinning its essential role in intracellular transport processes.

Biological function summary

The USO1 protein plays an instrumental role in maintaining Golgi structure and function. It belongs to the transport protein particle (TRAPP) complex and works closely with several other proteins in the vesicle tethering and fusion steps. By interacting with the Golgi SNAREs USO1 helps mediate the fusion process necessary for protein and lipid transport.

Pathways

USO1 integrates into cellular trafficking and transport pathways. It participates in the Golgi transport pathway working alongside proteins like GM130 and NSF to ensure stabilized vesicle docking and fusion. USO1's function connects it to the secretory pathway where it assists in uniting the endoplasmic reticulum's production line with the Golgi's packaging and shipping center.

Associated diseases and disorders

Abnormalities in USO1 function associate with neurological disorders and cancer progressions. USO1's interaction with GM130 links its dysfunctional activity to neuronal migration defects. Additionally aberrations in USO1 levels can influence cancer cell proliferation denoting its potential as a target for therapeutic strategies.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-USO1 antibody (ab184014), expandable thumbnail

    Western blot - Anti-USO1 antibody (ab184014)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-USO1 antibody (ab184014) at 1/1000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 108 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-USO1 antibody (ab184014), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-USO1 antibody (ab184014)

    Immunofluorescence analysis of methanol fixed A431 (human epidermoid carcinoma cell line) cells labelling USO1 in the Golgi apparatus with ab184014 at 1/500 dilution (green) and counterstained with Hoechst 33342. Cells were fixed in 100% methanol for 5 mins.

  • Western blot - Anti-USO1 antibody (ab184014), expandable thumbnail

    Western blot - Anti-USO1 antibody (ab184014)

    5% SDS-PAGE

    All lanes: Western blot - Anti-USO1 antibody (ab184014) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

    Lane 2: C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate at 30 µg

    Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

    Lane 5: C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

    Predicted band size: 108 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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