JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB264222

Anti-USP1 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal USP1 antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Ubiquitin carboxyl-terminal hydrolase 1 aa 550-650.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1, Ubiquitin thioesterase 1, Ubiquitin-specific-processing protease 1, hUBP, USP1

1 Images
Immunoprecipitation - Anti-USP1 antibody (AB264222)
  • IP

Supplier Data

Immunoprecipitation - Anti-USP1 antibody (AB264222)

USP1 was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264222 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using an alternative anti USP1 antibody at 1 μg/ml.

Lane 1 : USP1 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-USP1 antibody (ab264222)

Predicted band size: 88 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human Ubiquitin carboxyl-terminal hydrolase 1 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

O94782

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP1 also known as ubiquitin-specific peptidase 1 is a deubiquitinating enzyme with a molecular mass of approximately 88 kDa. It removes ubiquitin from protein substrates especially those involved in DNA repair processes. USP1 is widely expressed in human tissues with high expression in the testis and moderate levels in the lungs brain and heart. It forms a complex with UAF1 which enhances its catalytic activity and stability.
Biological function summary

USP1 plays a critical part in maintaining genomic stability by regulating key proteins in the DNA damage response. It specifically deubiquitinates FANCD2 and PCNA proteins important for the Fanconi anemia pathway and translesion DNA synthesis respectively. This action prevents the accumulation of ubiquitinated proteins ensuring proper progression of DNA repair processes. The interaction with UAF1 forms a functional complex important for its activity in vivo.

Pathways

USP1 is significant in the Fanconi anemia (FA) and translesion synthesis pathways. Its interaction with FANCD2 involves DNA repair by promoting error-free replication across DNA lesions. USP1 also acts on proliferating cell nuclear antigen (PCNA) a protein important for translesion synthesis that enables DNA replication to occur past unrepaired lesions. These pathways highlight USP1’s importance in DNA repair and replication linking it to other proteins like RAD18 which is involved in PCNA ubiquitination.

Research implicates USP1 in cancer and Fanconi anemia. In certain cancers altered USP1 expression can lead to increased DNA repair activity contributing to chemoresistance. USP1’s interaction with FANCD2 makes it pertinent to Fanconi anemia where its malfunction can cause DNA repair defects associated with the disease. Understanding USP1’s role alongside proteins like BRCA1 which interacts with DNA damage repair mechanisms can guide targeted therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed : 15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed : 16531995, PubMed : 20147293). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed : 18082604, PubMed : 26388029).
See full target information Ubiquitin carboxyl-terminal hydrolase 1
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB227551

Anti-USP1 antibody - C-terminal

Western blot - Anti-USP1 antibody - C-terminal (AB227551)

  • 0
  • 0
Primary Antibodies

AB84772

Anti-USP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP1 antibody (AB84772)

  • 0
  • 0
Primary Antibodies

AB264221

Anti-USP1 antibody

Immunoprecipitation - Anti-USP1 antibody (AB264221)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB112417

Recombinant Human RAD18 protein

SDS-PAGE - Recombinant Human RAD18 protein (AB112417)

  • 0
  • 0
Primary Antibodies

AB70800

Anti-ARTD10 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTD10 antibody (AB70800)

  • 0
  • 0
Primary Antibodies

AB313628

Anti-TRIP12/ULF antibody [EPR27062-86]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] (AB313628)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com