AB227551

Anti-USP1 antibody - C-terminal

Name: Anti-USP1 antibody - C-terminal (ab227551) | Abcam
Brand: Abcam Limited
SKU: AB227551
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal USP1 antibody. C-terminal. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Ubiquitin carboxyl-terminal hydrolase 1.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1, Ubiquitin thioesterase 1, Ubiquitin-specific-processing protease 1, hUBP, USP1

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human Ubiquitin carboxyl-terminal hydrolase 1. The exact immunogen used to generate this antibody is proprietary information.

O94782

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP1 also known as ubiquitin-specific peptidase 1 is a deubiquitinating enzyme with a molecular mass of approximately 88 kDa. It removes ubiquitin from protein substrates especially those involved in DNA repair processes. USP1 is widely expressed in human tissues with high expression in the testis and moderate levels in the lungs brain and heart. It forms a complex with UAF1 which enhances its catalytic activity and stability.

Biological function summary

USP1 plays a critical part in maintaining genomic stability by regulating key proteins in the DNA damage response. It specifically deubiquitinates FANCD2 and PCNA proteins important for the Fanconi anemia pathway and translesion DNA synthesis respectively. This action prevents the accumulation of ubiquitinated proteins ensuring proper progression of DNA repair processes. The interaction with UAF1 forms a functional complex important for its activity in vivo.

Pathways

USP1 is significant in the Fanconi anemia (FA) and translesion synthesis pathways. Its interaction with FANCD2 involves DNA repair by promoting error-free replication across DNA lesions. USP1 also acts on proliferating cell nuclear antigen (PCNA) a protein important for translesion synthesis that enables DNA replication to occur past unrepaired lesions. These pathways highlight USP1’s importance in DNA repair and replication linking it to other proteins like RAD18 which is involved in PCNA ubiquitination.

Research implicates USP1 in cancer and Fanconi anemia. In certain cancers altered USP1 expression can lead to increased DNA repair activity contributing to chemoresistance. USP1’s interaction with FANCD2 makes it pertinent to Fanconi anemia where its malfunction can cause DNA repair defects associated with the disease. Understanding USP1’s role alongside proteins like BRCA1 which interacts with DNA damage repair mechanisms can guide targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed : 15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed : 16531995, PubMed : 20147293). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed : 18082604, PubMed : 26388029).

See full target information Ubiquitin carboxyl-terminal hydrolase 1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 24:385 PubMed39568023

2024

Adipose stem cell exosomes, stimulated by pro-inflammatory factors, enhance immune evasion in triple-negative breast cancer by modulating the HDAC6/STAT3/PD-L1 pathway through the transporter UCHL1.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Zhu,Kejing Zhang,Yukun Cao,Yu Hu

Journal of cellular and molecular medicine 25:6535-6549 PubMed34046994

2021

TMEM161B-AS1 suppresses proliferation, invasion and glycolysis by targeting miR-23a-3p/HIF1AN signal axis in oesophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zuxuan Shi,Guanghui Li,Zhen Li,Junhao Liu,Yu Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-USP1 antibody - C-terminal

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise