Anti-USP10 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP10 antibody (AB70895)

IHC image of ab70895 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70895, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP10 antibody (AB70895)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling USP10 with ab70895 at 1/200 (1 μg/ml). Detection : DAB.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-USP10 antibody (AB70895)

ICC image of ab70895 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70895, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• IP

Supplier Data

Immunoprecipitation - Anti-USP10 antibody (AB70895)

Detection of human USP10 by western blot of Immunoprecipitates.
Samples : Lane 1 is whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer and Lane 2 is control IgG.
Antibodies : ab70895 used for IP at 3 μg per reaction. For blotting immunoprecipitated USP10, ab70895 was used at 1 μg/ml.

All lanes:

Immunoprecipitation - Anti-USP10 antibody (ab70895)

Predicted band size: 87 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-USP10 antibody (AB70895)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-USP10 antibody (ab70895) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 87 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-USP10 antibody (AB70895)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-USP10 antibody (ab70895) at 0.1 µg/mL

All lanes:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Predicted band size: 87 kDa

false

Exposure time: 3min