Rabbit Polyclonal USP10 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human USP10 aa 50-100.
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- Journal of orthopaedic surgery and research 18:8202023Carbamazepine regulates USP10 through miR-20a-5p to affect the deubiquitination of SKP2 and inhibit osteogenic differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Wang,Ziye Liu,Dawei Niu,Haobo Li,Yaguang Han,Jinhui Peng,Qirong QianPubMed 37915040
- Frontiers in cell and developmental biology 9:7570682021FSTL1-USP10-Notch1 Signaling Axis Protects Against Cardiac Dysfunction Through Inhibition of Myocardial Fibrosis in Diabetic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinhe Lu,Jipeng Ma,Yang Liu,Yalan Shao,Xiang Xiong,Weixun Duan,Erhe Gao,Qianli Yang,Shasha Chen,Jian Yang,Jun Ren,Qijun Zheng,Jincheng LiuPubMed 34957094
- Current biology : CB 30:698-707.e62020UBAP2L Forms Distinct Cores that Act in Nucleating Stress Granules Upstream of G3BP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Cirillo et. al.PubMed 31956030
- Journal of virology 87:489-5022012Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroshi Katoh,Toru Okamoto,Takasuke Fukuhara,Hiroto Kambara,Eiji Morita,Yoshio Mori,Wataru Kamitani,Yoshiharu MatsuuraPubMed 23097442
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human USP10 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q14694
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
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Target data
Function
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, RPS2/us5, RPS3/us3, RPS10/eS10, BECN1, SNX3 and CFTR (PubMed:11439350, PubMed:18632802, PubMed:31981475). Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53 (PubMed:20096447). Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response (PubMed:20096447). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes (PubMed:21962518). In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13 (PubMed:21962518). Does not deubiquitinate MDM2 (PubMed:20096447). Plays a key role in 40S ribosome subunit recycling when a ribosome has stalled during translation: acts both by inhibiting formation of stress granules, which store stalled translation pre-initiation complexes, and mediating deubiquitination of 40S ribosome subunits (PubMed:27022092, PubMed:31981475, PubMed:34348161, PubMed:34469731). Acts as a negative regulator of stress granules formation by lowering G3BP1 and G3BP2 valence, thereby preventing G3BP1 and G3BP2 ability to undergo liquid-liquid phase separation (LLPS) and assembly of stress granules (PubMed:11439350, PubMed:27022092, PubMed:32302570). Promotes 40S ribosome subunit recycling following ribosome dissociation in response to ribosome stalling by mediating deubiquitination of 40S ribosomal proteins RPS2/us5, RPS3/us3 and RPS10/eS10, thereby preventing their degradation by the proteasome (PubMed:31981475, PubMed:34348161, PubMed:34469731). Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC): USP10 acts by removing monoubiquitination of RPS2/us5 and RPS3/us3, promoting 40S ribosomal subunit recycling (PubMed:34469731). Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling (PubMed:19398555). Involved in a TANK-dependent negative feedback response to attenuate NF-kappa-B activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989). Deubiquitinates TBX21 leading to its stabilization (PubMed:24845384). Plays a negative role in the RLR signaling pathway upon RNA virus infection by blocking the RIGI-mediated MAVS activation. Mechanistically, removes the unanchored 'Lys-63'-linked polyubiquitin chains of MAVS to inhibit its aggregation, essential for its activation (PubMed:37582970).
Alternative names
KIAA0190, USP10, Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10, Ubiquitin thioesterase 10, Ubiquitin-specific-processing protease 10
Recommended products
Rabbit Polyclonal USP10 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human USP10 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human USP10 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q14694
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP10 also known as ubiquitin-specific peptidase 10 is a deubiquitinating enzyme with a mass of approximately 94 kDa. It primarily functions to cleave ubiquitin from protein substrates controlling protein degradation localization and activity. USP10 is widely expressed in various tissues with significant expression in the cytoplasm. This enzyme helps maintain protein homeostasis and regulates various cellular processes such as cell cycle progression and apoptosis.
- Biological function summary
Ubiquitin-specific peptidase 10 serves an important role in stabilizing and regulating proteins. USP10 interacts with and stabilizes several substrates including p53 an important tumor suppressor. It often operates in concert with other proteins as part of several protein complexes enabling cellular responses to stress. The enzyme's ability to control the stability of multiple proteins allows it to influence cell survival and DNA repair mechanisms significantly.
- Pathways
USP10 plays a significant role in pivotal pathways such as the p53 signaling pathway and the NF-kB signaling pathway. It interacts closely with proteins like MDM2 a major regulator of p53 directly affecting p53's stability and activity. In the NF-kB pathway USP10 regulates the deubiquitination of receptor-interacting serine/threonine-protein kinase 1 (RIPK1) playing a part in controlling immune responses and inflammation.
- Associated diseases and disorders
The activity of USP10 impacts cancer progression and neurodegenerative conditions like Alzheimer's disease. In cancer alterations in USP10 levels can lead to abnormal p53 regulation which can facilitate tumorigenesis. Regarding Alzheimer's disease USP10 may influence the aggregation and accumulation of proteins such as tau linked to neurodegenerative pathology by regulating their degradation pathway.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-USP10 antibody (ab70895)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-USP10 antibody (ab70895) at 0.1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 87 kDa
Exposure time: 30s
Immunoprecipitation - Anti-USP10 antibody (ab70895)
Detection of human USP10 by western blot of Immunoprecipitates.
Samples: Lane 1 is whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer and Lane 2 is control IgG.
Antibodies: ab70895 used for IP at 3 μg per reaction. For blotting immunoprecipitated USP10, ab70895 was used at 1 μg/ml.All lanes: Immunoprecipitation - Anti-USP10 antibody (ab70895)
Predicted band size: 87 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP10 antibody (ab70895)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling USP10 with ab70895 at 1/200 (1 μg/ml). Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-USP10 antibody (ab70895)
ICC image of ab70895 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70895, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Anti-USP10 antibody (ab70895)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-USP10 antibody (ab70895) at 0.1 µg/mL
All lanes: TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg
Predicted band size: 87 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP10 antibody (ab70895)
IHC image of ab70895 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70895, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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