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Rabbit Recombinant Monoclonal USP11 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 18 publications.


Images

Immunoprecipitation - Anti-USP11 antibody [EPR4346] (AB109232), expandable thumbnail
  • Western blot - Anti-USP11 antibody [EPR4346] (AB109232), expandable thumbnail
  • Immunoprecipitation - Anti-USP11 antibody [EPR4346] (AB109232), expandable thumbnail
  • Western blot - Anti-USP11 antibody [EPR4346] (AB109232), expandable thumbnail
  • Western blot - Anti-USP11 antibody [EPR4346] (AB109232), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Expected
Tested
Not recommended
Not recommended
Rat
Expected
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/10 - 1/300
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed:12084015, PubMed:15314155, PubMed:17897950, PubMed:19874889, PubMed:20233726, PubMed:24724799, PubMed:28992046). Inhibits the degradation of target proteins by the proteasome (PubMed:12084015). Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro) (PubMed:24724799). Plays a role in the regulation of pathways leading to NF-kappa-B activation (PubMed:17897950, PubMed:19874889). Plays a role in the regulation of DNA repair after double-stranded DNA breaks (PubMed:15314155, PubMed:20233726). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (PubMed:20601937). Promotes cell proliferation by deubiquitinating phosphorylated E2F1 (PubMed:28992046).

Alternative names

UHX1, USP11, Ubiquitin carboxyl-terminal hydrolase 11, Deubiquitinating enzyme 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11

Recommended products

Rabbit Recombinant Monoclonal USP11 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 18 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4346
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

USP11 also known as ubiquitin specific peptidase 11 is a deubiquitinating enzyme with a molecular mass of approximately 110 kDa. It removes ubiquitin molecules from target proteins regulating their stability and function. This enzyme is part of the USP family and exhibits strong expression in tissues like the testes brain and thyroid. The deubiquitination process is significant in maintaining protein homeostasis by recycling ubiquitins and controlling protein degradation.

Biological function summary

Ubiquitin-specific peptidases such as USP11 participate in diverse cellular functions including DNA repair signal transduction and cell cycle progression. USP11 often operates as part of a larger regulatory complex. It modulates DNA repair by interacting with proteins involved in the repair processes such as BRCA2. Through its deubiquitination activity USP11 significantly influences cellular response to DNA damage and helps to maintain genomic stability.

Pathways

USP11 plays a role in key mechanisms like the DNA damage response and NF-kB signaling pathways. It interacts with proteins involved in these pathways offering a layer of regulation. In the DNA damage response pathway USP11 associates with BRCA2 assisting in the repair of double-stranded breaks. In the NF-kB signaling pathway its activity can affect the degradation of IkB which allows the transcription factor NF-kB to enter the nucleus and promote gene expression.

Associated diseases and disorders

Aberrations in USP11 function have been linked to cancer and neurodegenerative diseases. In cancer alterations in USP11 might contribute to tumorigenesis by affecting the DNA repair pathways especially through the BRCA2 protein interactions. Neurodegenerative diseases may also be connected to USP11 dysfunction due to its role in the degradation of proteins that accumulate in these conditions. The enzyme's interaction with various signaling proteins suggests a potential target for therapeutic interventions in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232), expandable thumbnail

    Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)

    ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg HEK293 (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)

    Predicted band size: 110 kDa

  • Western blot - Anti-USP11 antibody [EPR4346] (ab109232), expandable thumbnail

    Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: USP11 knockout HAP1 cell lysate (20 μg)
    Lane 3: LNCaP cell lysate (20 μg)
    Lane 4: HeLa cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109232 observed at 110 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab109232 was shown to specifically react with USP11 when USP11 knockout samples were used. Wild-type and USP11 knockout samples were subjected to SDS-PAGE. ab109232 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

    Predicted band size: 110 kDa

  • Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232), expandable thumbnail

    Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)

    ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg Jurkat (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)

    Predicted band size: 110 kDa

  • Western blot - Anti-USP11 antibody [EPR4346] (ab109232), expandable thumbnail

    Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution

    Lane 1: mouse brain lysate at 20 µg

    Lane 2: rat brain lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1000 µg

    Predicted band size: 110 kDa

    Observed band size: 110 kDa

  • Western blot - Anti-USP11 antibody [EPR4346] (ab109232), expandable thumbnail

    Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution

    Lane 1: HEK293 lysate at 20 µg

    Lane 2: human fetal kidney lysate

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 110 kDa, 115 kDa, 132 kDa, 27 kDa, 53 kDa

    Observed band size: 110 kDa, 116 kDa, 135 kDa, 35 kDa

  • Western blot - Anti-USP11 antibody [EPR4346] (ab109232), expandable thumbnail

    Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution

    Lane 1: Jurkat lysate at 20 µg

    Lane 2: human testis lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 110 kDa, 28 kDa

    Observed band size: 110 kDa

  • Western blot - Anti-USP11 antibody [EPR4346] (ab109232), expandable thumbnail

    Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

    All lanes: Western blot - Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: Human testis cell lysate at 10 µg

    Lane 4: Huma fetal kidney cell lysate at 10 µg

    Lane 5: LnCaP cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 110 kDa

    Observed band size: 110 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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