Rabbit Recombinant Monoclonal USP13 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Images
![Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (AB247812), expandable thumbnail](https://content.abcam.com/products/images/usp13-antibody-epr4348-bsa-and-azide-free-ab247812--western-blot-img99682.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (AB247812)")
![Flow Cytometry (Intracellular) - Anti-USP13 antibody [EPR4348] - BSA and Azide free (AB247812), expandable thumbnail](https://content.abcam.com/products/images/usp13-antibody-epr4348-bsa-and-azide-free-ab247812--flow-cytometry-intracellular-img140006.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-USP13 antibody [EPR4348] - BSA and Azide free (AB247812)")
![Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (AB247812), expandable thumbnail](https://content.abcam.com/products/images/usp13-antibody-epr4348-bsa-and-azide-free-ab247812--western-blot-img106282.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (AB247812)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested |
| Mouse | Predicted | Not recommended | Expected | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy, endoplasmic reticulum-associated degradation (ERAD), cell cycle progression or DNA damage response (PubMed:21571647, PubMed:32772043, PubMed:33592542). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Alternatively, forms with NEDD4 a deubiquitination complex, which subsequently stabilizes VPS34 to promote autophagy (PubMed:32101753). Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Regulates the cell cycle progression by stabilizing cell cycle proteins such as SKP2 and AURKB (PubMed:32772043). In addition, plays an important role in maintaining genomic stability and in DNA replication checkpoint activation via regulation of RAP80 and TOPBP1 (PubMed:33592542). Deubiquitinates the multifunctional protein HMGB1 and subsequently drives its nucleocytoplasmic localization and its secretion (PubMed:36585612). Positively regulates type I and type II interferon signalings by deubiquitinating STAT1 but negatively regulates antiviral response by deubiquitinating STING1 (PubMed:23940278, PubMed:28534493).
Alternative names
ISOT3, USP13, Ubiquitin carboxyl-terminal hydrolase 13, Deubiquitinating enzyme 13, Isopeptidase T-3, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, ISOT-3
Recommended products
Rabbit Recombinant Monoclonal USP13 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR4348
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247812 is the carrier-free version of Anti-USP13 antibody [EPR4348] ab109264.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP13 also known as Ubiquitin Specific Protease 13 functions as a deubiquitinating enzyme. It is important in removing ubiquitin molecules from protein substrates which prevents them from being degraded by the proteasome system. USP13 has a molecular mass of approximately 97 kDa and shows expression in several tissues including brain heart and kidney. This indicates its diverse role in human physiology.
- Biological function summary
USP13 participates in maintaining protein homeostasis and modulates protein stability. It forms complexes with specific substrate proteins to execute its function efficiently. Through deubiquitinating actions it regulates the levels of proteins involved in cellular signaling apoptosis and cell cycle control. The enzyme influences processes that are important for cell survival and adaptation to stress.
- Pathways
USP13 plays significant roles within the ubiquitin-proteasome system and the mitophagy pathway. It cooperates with proteins like Parkin in mitochondrial quality control by stabilizing target proteins necessary for mitophagy. Through interaction with key players in these pathways USP13 ensures the maintenance of cellular health and proper degradation of damaged or misfolded proteins.
- Associated diseases and disorders
USP13 has associations with cancer and neurodegenerative diseases. Overexpression of USP13 might contribute to tumor progression by inhibiting degradation of oncoproteins. It also shows connections to disorders like Parkinson's disease where it interacts with proteins such as Parkin and PINK1 which are important for mitochondrial function. These interactions highlight USP13's potential as a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812), expandable thumbnail](https://content.abcam.com/products/images/usp13-antibody-epr4348-bsa-and-azide-free-ab247812--western-blot-img99682.jpg "Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)")
Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)
This data was developed using Anti-USP13 antibody [EPR4348] ab109264, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: USP13 knockout HAP1 cell lysate (20 μg)
Lane 3: A375 cell lysate (20 μg)
Lane 4: SH-SY5Y cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-USP13 antibody [EPR4348] ab109264 observed at 100 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-USP13 antibody [EPR4348] ab109264 was shown to recognize USP13 when USP13 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP13 knockout samples were subjected to SDS-PAGE. Anti-USP13 antibody [EPR4348] ab109264 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-USP13 antibody [EPR4348] (Anti-USP13 antibody [EPR4348] ab109264)
Predicted band size: 97 kDa
![Flow Cytometry (Intracellular) - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812), expandable thumbnail](https://content.abcam.com/products/images/usp13-antibody-epr4348-bsa-and-azide-free-ab247812--flow-cytometry-intracellular-img140006.jpg "Flow Cytometry (Intracellular) - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)")
Flow Cytometry (Intracellular) - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)
This data was developed using Anti-USP13 antibody [EPR4348] ab109264, the same antibody clone in a different buffer formulation.Overlay histogram showing SHSY-5Y cells stained with Anti-USP13 antibody [EPR4348] ab109264 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-USP13 antibody [EPR4348] ab109264, 1/9400 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812), expandable thumbnail](https://content.abcam.com/products/images/usp13-antibody-epr4348-bsa-and-azide-free-ab247812--western-blot-img106282.jpg "Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)")
Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)
This data was developed using Anti-USP13 antibody [EPR4348] ab109264, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-USP13 antibody [EPR4348] (Anti-USP13 antibody [EPR4348] ab109264) at 1/1000 dilution
Lane 1: SH SY5Y cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: A375 cell lysate at 10 µg
Predicted band size: 97 kDa
Observed band size: 97 kDa
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