AB137432

Anti-USP14/TGT antibody

Name: Anti-USP14/TGT antibody (ab137432) | Abcam
Brand: Abcam Limited
SKU: AB137432
Rating: 2 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal USP14/TGT antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP14 aa 250 to C-terminus.

View Alternative Names

TGT, USP14, Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP14/TGT antibody (AB137432)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP14/TGT antibody (AB137432)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling USP14/TGT with ab137432 at 1/500 dilution.

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Western blot - Anti-USP14/TGT antibody (AB137432)

7.5% SDS PAGE

All lanes:

Western blot - Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

Jurkat whole cell lysate at 30 µg

Predicted band size: 56 kDa

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Supplier Data

Western blot - Anti-USP14/TGT antibody (AB137432)

7.5% SDS PAGE

All lanes:

Western blot - Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 56 kDa

false

Unknown

Western blot - Anti-USP14/TGT antibody (AB137432)

7.5 % SDS-PAGE

All lanes:

Western blot - Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution

All lanes:

Rat brain cell lyaste at 50 µg

Predicted band size: 56 kDa

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Lab

Western blot - Anti-USP14/TGT antibody (AB137432)

All lanes:

Western blot - Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution

Lane 1:

WT HeLa cell extract at 30 µg

Lane 2:

USP14/TGT Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human USP14 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54578

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP14 also known as Ubiquitin Specific Peptidase 14 is a deubiquitinating enzyme that plays a mechanical role in the ubiquitin-proteasome system. This enzyme has a molecular mass around 56 kDa and is essential for proteasome-mediated degradation activities. It is broadly expressed across many tissues but shows significant presence in the nervous system. Often people refer to USP14 as TGT in the context of certain studies or databases.

Biological function summary

USP14 functions in regulating protein turnover by removing ubiquitin from substrate proteins impacting their stability and degradation. USP14 is part of a larger protein complex associated with the proteasome specifically binding to the 19S regulatory particle. It is important in maintaining cellular protein homeostasis by editing ubiquitin chains on target proteins prior to degradation.

Pathways

USP14 participates in ubiquitin-proteasome pathways and ER-associated degradation pathway. In these processes it works closely with other proteasome proteins such as Rpn11 which also removes ubiquitin from proteins. The regulation of these pathways by USP14 directly affects protein quality control in cells influencing processes like cell cycle and stress response.

USP14 has associations with neurodegenerative diseases such as Alzheimer's and Parkinson’s. It affects disease progression by modulating levels of proteins that aggregate in these conditions. Connections between USP14 and proteins such as tau and α-synuclein highlight its contribution to the pathological features seen in these disorders. Further research into USP14's role may help to uncover new therapeutic strategies for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed : 35145029). Ensures the regeneration of ubiquitin at the proteasome (PubMed : 18162577, PubMed : 28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed : 18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed : 19106094). Also serves as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed : 19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed : 27666593). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (PubMed : 35145029).

See full target information USP14

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8030 PubMed40877267

2025

Organic-inorganic covalent selenium reversing ischemic reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zushuang Xiong,Guanning Huang,Jia-Run Huang,Ying Liu,Lizhen He,Tianfeng Chen

Acta biochimica et biophysica Sinica 54:1720 - 1730 PubMed36514221

2022

USP14 promotes pyroptosis of human annulus fibrosus cells derived from patients with intervertebral disc degeneration through deubiquitination of NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Hai,Tianli Mao,Chuanchao Du,Fei Jia,Yu Liu,Qingpeng Song,Xiaoyu Pan,Xiaoguang Liu,Bin Zhu

Artificial cells, nanomedicine, and biotechnology 47:3720-3728 PubMed31523993

2019

Matrine derivate MASM protects murine MC3T3-E1 osteoblastic cells against dexamethasone-induced apoptosis via the regulation of USP14/p53.

Applications

Unspecified application

Species

Unspecified reactive species

Panyu Zhou,Demeng Xia,Yang Wang,Hongdi Lv,Ziqin Wang,Malcolm Xing,Qingjie Zhao,Shuogui Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP14/TGT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP14/TGT antibody (AB137432)

Western blot - Anti-USP14/TGT antibody (AB137432)

Western blot - Anti-USP14/TGT antibody (AB137432)

Western blot - Anti-USP14/TGT antibody (AB137432)

Western blot - Anti-USP14/TGT antibody (AB137432)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise