Rabbit Polyclonal USP14/TGT antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human USP14 aa 350-400.
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- Proteomics 23:e22004322023Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan Hong,Xi Chen,Honggang Wang,Xiangqian Gu,Yin Yuan,Zixiang ZhangPubMed 36625413
- Annals of translational medicine 9:10192021The prognostic value of USP14 and PSMD14 expression in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Lei,Xiaoliu Liu,Wei Liu,Yun Zhang,Zhihong LiuPubMed 34277819
- Frontiers in cell and developmental biology 9:6477022021The mA Reader YTHDF1 Facilitates the Tumorigenesis and Metastasis of Gastric Cancer via USP14 Translation in an mA-Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Yu Chen,Rui Liang,You-Cai Yi,Hui-Ning Fan,Ming Chen,Jing Zhang,Jin-Shui ZhuPubMed 33791305
- PloS one 9:e869982014HIV-1 Nef down-modulates C-C and C-X-C chemokine receptors via ubiquitin and ubiquitin-independent mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrabha Chandrasekaran et. al.PubMed 24489825
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human USP14 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link P54578
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed:35145029). Ensures the regeneration of ubiquitin at the proteasome (PubMed:18162577, PubMed:28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed:19106094). Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed:19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed:27666593). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (PubMed:35145029).
Alternative names
TGT, USP14, Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14
Recommended products
Rabbit Polyclonal USP14/TGT antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human USP14 aa 350-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human USP14 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link P54578
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP14 also known as Ubiquitin Specific Peptidase 14 is a deubiquitinating enzyme that plays a mechanical role in the ubiquitin-proteasome system. This enzyme has a molecular mass around 56 kDa and is essential for proteasome-mediated degradation activities. It is broadly expressed across many tissues but shows significant presence in the nervous system. Often people refer to USP14 as TGT in the context of certain studies or databases.
- Biological function summary
USP14 functions in regulating protein turnover by removing ubiquitin from substrate proteins impacting their stability and degradation. USP14 is part of a larger protein complex associated with the proteasome specifically binding to the 19S regulatory particle. It is important in maintaining cellular protein homeostasis by editing ubiquitin chains on target proteins prior to degradation.
- Pathways
USP14 participates in ubiquitin-proteasome pathways and ER-associated degradation pathway. In these processes it works closely with other proteasome proteins such as Rpn11 which also removes ubiquitin from proteins. The regulation of these pathways by USP14 directly affects protein quality control in cells influencing processes like cell cycle and stress response.
- Associated diseases and disorders
USP14 has associations with neurodegenerative diseases such as Alzheimer's and Parkinson’s. It affects disease progression by modulating levels of proteins that aggregate in these conditions. Connections between USP14 and proteins such as tau and α-synuclein highlight its contribution to the pathological features seen in these disorders. Further research into USP14's role may help to uncover new therapeutic strategies for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-USP14/TGT antibody (ab71165)
All lanes: Western blot - Anti-USP14/TGT antibody (ab71165) at 0.1 µg/mL
Lane 1: Whole cell lysate from Hela cells at 50 µg
Lane 2: Whole cell lysate from Hela cells at 15 µg
Lane 3: Whole cell lysate from Hela cells at 5 µg
Lane 4: Whole cell lysate from 293T cells at 50 µg
Predicted band size: 56 kDa
Observed band size: 28 kDa, 40 kDa, 56 kDa
Immunoprecipitation - Anti-USP14/TGT antibody (ab71165)
Detection of Human USP14/TGT by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab71165 at 3 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
All lanes: Immunoprecipitation - Anti-USP14/TGT antibody (ab71165)
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP14/TGT antibody (ab71165)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP14 with ab71165 at 1/1000 (1µg/ml). Detection: DAB.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-USP14/TGT antibody (ab71165)
USP14/TGT western blot using anti-USP14/TGT antibody ab71165. Publication image and figure legend from Chandrasekaran, P., Moore, V., et al., 2014, PLoS One, PubMed 24489825.
ab71165 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab71165 please see the product overview.
siRNA knockdown of AP2, E3 ubiquitin ligases, AIP4 and NEDD4 and Hrs/Vps27, a candidate ESCRT-0 protein reversed Nef induced downregulation of CXCR4 or CCR5. A) Histograms of relative (%) MFVs (with standard deviation) of native CXCR4 (left) in Jurkat cells or CCR5 in CEM cell line (right) expressing Nef and GFP are shown in the context of siRNA knockdown of AIP4, AP2, NEDD4 and HRS (*p<0.05 compared with Nef and mock siRNA transfected cells). B) Nef induced CXCR4 downregulation was not reversed by siRNA knockdown of AP1, clathrin, β-arrestin, deubiquitinases, AMSH, STAM, and USP14 and a candidate ESCRT adapter, TSG101/Vps23P. CD4 downregulation by Nef was resistant to all siRNA knockdowns except for clathrin (B, right, n = 5; *p<0.01). Expression levels of proteins targeted by cognate siRNAs were monitored by immuno-blots shown underneath panels A and B.
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