AB71713

Anti-USP15 antibody

Name: Anti-USP15 antibody (ab71713) | Abcam
Brand: Abcam Limited
SKU: AB71713
Price: 545 USD
Availability: InStock

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(14 Publications)

Rabbit Polyclonal USP15 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human USP15.

View Alternative Names

KIAA0529, USP15, Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4

3 Images

Supplier Data

Immunoprecipitation - Anti-USP15 antibody (AB71713)

USP15 was immunoprecipitated from HeLa whole cell lysate with ab71713 at 6 μg (1.0 mg per IP reaction; 20% of IP loaded).
Lane 1 : ab71713
Lane 2 : ab71713 at 6 μg per reaction.
Lane 3 : Control IgG
Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-USP15 antibody (ab71713)

Predicted band size: 112 kDa

false

Supplier Data

Western blot - Anti-USP15 antibody (AB71713)

All lanes:

Western blot - Anti-USP15 antibody (ab71713) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

K-562 whole cell lysate at 50 µg

Lane 3:

MCF-7 whole cell lysate at 50 µg

Lane 4:

U2OS whole cell lysate at 50 µg

Lane 5:

RKO whole cell lysate at 50 µg

Lane 6:

TCMK-1 whole cell lysate at 50 µg

Lane 7:

NIH/3T3 whole cell lysate

Predicted band size: 112 kDa

false

CiteAb

Western blot - Anti-USP15 antibody (AB71713)

Western Blotting using Anti-USP15 antibody, ab71713. Publication image from Zhou, L. et al., 2018, Exp Mol Med, 30459344. Legend direct from paper.

USP15 expression in MM tissues and cell lines.a The mRNA expression of USP15 in bone marrow samples from 80 patients with multiple myeloma (MM) and 15 patients with proliferative bone marrow (PBM) was measured by Real-time PCR. The expression of USP15 in five different MM cell lines and in control cells, non-cancerous bone marrow-derived plasma cells, was measured by Real-time PCR (b) and western blotting (c). **P < 0.01 compared with PBM or control

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human USP15. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4E8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP15 also known as Ubiquitin Specific Peptidase 15 is a deubiquitinating enzyme that removes ubiquitin from target proteins a process integral to protein degradation and regulation. It has a molecular mass of approximately 107 kDa. USP15 regulates protein turnover and stability through its enzymatic activity and is predominantly cytoplasmic expressed in various tissues including the brain heart and liver. Its expression pattern suggests widespread functionality across different physiological contexts.

Biological function summary

USP15 is involved in numerous cellular processes through its role in protein homeostasis. It associates with several protein complexes to regulate signaling pathways and cellular responses. USP15 plays a central role in the regulation of TGF-β signaling by deubiquitinating and stabilizing SMAD proteins that are important for signal transduction. In addition to TGF-β modulation USP15 also participates in endocytic trafficking by associating with the ESCRT complex impacting receptor degradation and cell signaling.

Pathways

The function of USP15 is critical in integrating cellular signaling networks such as the TGF-β and NF-kB pathways. Within the TGF-β pathway USP15 deubiquitinates receptor-regulated SMADs maintaining their stability and enhancing signal transduction. In the NF-kB pathway USP15 influences the activation by regulating the degradation of IκBα an inhibitor of NF-kB which indicates its role in immune response and inflammation. Through these pathways USP15 interacts with proteins like SMAD2/3 in TGF-β signaling and IκBα in NF-kB regulation.

USP15 has associations with cancer and neurodegenerative diseases. In cancer altered USP15 activity leads to dysregulation of TGF-β signaling influencing tumor progression and metastasis. The enzyme interacts with proteins such as MDM2 in neurodegenerative disorders affecting p53 stability and neuronal cell survival. This suggests a role in processes like apoptosis and DNA repair critical in the onset and progression of neurodegenerative conditions. But current research continues aiming for better understanding of USP15's connections with these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004, PubMed : 21947082, PubMed : 22344298, PubMed : 24852371). Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear : according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed : 21947082). According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal (PubMed : 22344298). Able to mediate deubiquitination of monoubiquitinated substrates, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 33093067). May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B (PubMed : 24526689). Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN) : hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy (PubMed : 24852371). Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association : regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004). Involved in endosome organization by mediating deubiquitination of SQSTM1 : ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery (PubMed : 27368102). Acts as a negative regulator of antifungal immunity by mediating 'Lys-27'-linked deubiquitination of CARD9, thereby inactivating CARD9 (PubMed : 33093067).. (Microbial infection) Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.

See full target information USP15

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:336 PubMed40176111

2025

USP15-modified ADMSCs-Exo alleviates chondrocyte damage and effectively relieved osteoarthritis by inducing M2 polarization of macrophages through deubiquitinating FOXC1.

Applications

Unspecified application

Species

Unspecified reactive species

Qibin Liang,Qinghe Ding,Liang Zhao,Jingchao Tan,Wei Niu

Clinical and translational medicine 15:e70118 PubMed39731281

2024

The antiarthritic effect of CBR-470-1 in hypoxic environment is to increase the level of NOD-like receptor family pyrin domain containing 3 ubiquitination by decreasing phosphoglycerate kinase 1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ao Duan,Zemeng Ma,Xiaolong Shao,Zhencheng Xiong,Chaoyi Zhang,Wenzheng Liu,Guanglin Wang,Shouye Hu,Wei Lin

Aging 16:6757-6772 PubMed38656882

2024

USP15 facilitates the progression of bladder cancer by amplifying the activation of the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Li,Chenghang Jiang,Quanqi Liu,Pengfei Zhou,Daxue Tian,Ying Zeng,Mingfeng Xiang

British journal of cancer 130:526-541 PubMed38182686

2024

TRIM21/USP15 balances ACSL4 stability and the imatinib resistance of gastrointestinal stromal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Cui,Haoyu Sun,Zhishuang Gao,Chao Li,Tingting Xiao,Yibo Bian,Zonghang Liu,Tianhao Gu,Jianan Zhang,Tengyun Li,Qianzheng Zhou,Zhongyuan He,Bowen Li,Fengyuan Li,Zekuan Xu,Hao Xu

Journal of oncology 2022:1427726 PubMed36213818

2022

A Regulatory Network Analysis of the Importance of USP15 in Breast Cancer Metastasis and Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ling,Chenhui Qin,Tao Li,Baozhen Wang,Weiji Cai,Lei Ma,Yanfeng Wang,Jing Chen,Fang Xu

iScience 25:104952 PubMed36065190

2022

GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yang,Xiaocen Liu,Xiaolong Zhu,Mengying Zhang,Yingying Wang,Mingzhe Ma,Kun Lv

Plastic and reconstructive surgery 148:1040-1051 PubMed34546211

2021

USP15 Enhances the Proliferation, Migration, and Collagen Deposition of Hypertrophic Scar-Derived Fibroblasts by Deubiquitinating TGF-βR1 In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Longxiang Tu,Zunwen Lin,Qin Huang,Dewu Liu

Oxidative medicine and cellular longevity 2021:9674809 PubMed34422211

2021

Platelet-Rich Plasma-Derived Exosomal USP15 Promotes Cutaneous Wound Healing via Deubiquitinating EIF4A1.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xu,Ze Lin,Lei He,Yanzhen Qu,Liu Ouyang,Yu Han,Chao Xu,Deyu Duan

Arthritis research & therapy 23:84 PubMed33726807

2021

Positive feedback regulation between USP15 and ERK2 inhibits osteoarthritis progression through TGF-β/SMAD2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Wang,Yanhui Zhu,Zhenyu Sun,Chen Jin,Xiang Wang

Cell reports 33:108533 PubMed33378683

2020

USP15 Deubiquitinase Safeguards Hematopoiesis and Genome Integrity in Hematopoietic Stem Cells and Leukemia Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Paul van den Berk,Cesare Lancini,Carlos Company,Michela Serresi,Maria Pilar Sanchez-Bailon,Danielle Hulsman,Colin Pritchard,Ji-Ying Song,Matthias Jürgen Schmitt,Ellen Tanger,Oliver Popp,Philipp Mertins,Ivo J Huijbers,Heinz Jacobs,Maarten van Lohuizen,Gaetano Gargiulo,Elisabetta Citterio

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-USP15 antibody

Immunoprecipitation - Anti-USP15 antibody (AB71713)

Western blot - Anti-USP15 antibody (AB71713)

Western blot - Anti-USP15 antibody (AB71713)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise