Rabbit Polyclonal USP15 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human USP15 aa 900 to C-terminus.
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Publications
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- Experimental & molecular medicine 55:1520-15302023Ablation of the deubiquitinase USP15 ameliorates nonalcoholic fatty liver disease and nonalcoholic steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung-Hwan Baek,Myung Sup Kim,Hye Ryeon Jung,Min-Seon Hwang,Chan-Ho Lee,Dai Hoon Han,Yong-Ho Lee,Eugene C Yi,Seung-Soon Im,Ilseon Hwang,Kyungeun Kim,Joon-Yong Chung,Kyung-Hee ChunPubMed 37394587
- iScience 25:1049522022GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Yang,Xiaocen Liu,Xiaolong Zhu,Mengying Zhang,Yingying Wang,Mingzhe Ma,Kun LvPubMed 36065190
- Cell death & disease 13:3482022USP15 negatively regulates lung cancer progression through the TRAF6-BECN1 signaling axis for autophagy induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMi-Jeong Kim,Yoon Min,Soo-Kyung Jeong,Juhee Son,Ji Young Kim,Ji Su Lee,Duk-Hwan Kim,Joo Sang Lee,Eunyoung Chun,Ki-Young LeePubMed 35422093
- Structure (London, England : 1993) 27:590-605.e52019Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoan Teyra et. al.PubMed 30713027
- Nature medicine 18:429-352012USP15 stabilizes TGF-β receptor I and promotes oncogenesis through the activation of TGF-β signaling in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPieter J A Eichhorn et. al.PubMed 22344298
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human USP15 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4E8
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/250.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Associated Products
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Target data
Function
Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways (PubMed:16005295, PubMed:17318178, PubMed:19576224, PubMed:19826004, PubMed:21947082, PubMed:22344298, PubMed:24852371). Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed:21947082). According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal (PubMed:22344298). Able to mediate deubiquitination of monoubiquitinated substrates, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:33093067). May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B (PubMed:24526689). Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy (PubMed:24852371). Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP (PubMed:16005295, PubMed:17318178, PubMed:19576224, PubMed:19826004). Involved in endosome organization by mediating deubiquitination of SQSTM1: ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery (PubMed:27368102). Acts as a negative regulator of antifungal immunity by mediating 'Lys-27'-linked deubiquitination of CARD9, thereby inactivating CARD9 (PubMed:33093067). (Microbial infection) Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
Alternative names
KIAA0529, USP15, Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4
Recommended products
Rabbit Polyclonal USP15 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human USP15 aa 900 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human USP15 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4E8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP15 also known as Ubiquitin Specific Peptidase 15 is a deubiquitinating enzyme that removes ubiquitin from target proteins a process integral to protein degradation and regulation. It has a molecular mass of approximately 107 kDa. USP15 regulates protein turnover and stability through its enzymatic activity and is predominantly cytoplasmic expressed in various tissues including the brain heart and liver. Its expression pattern suggests widespread functionality across different physiological contexts.
- Biological function summary
USP15 is involved in numerous cellular processes through its role in protein homeostasis. It associates with several protein complexes to regulate signaling pathways and cellular responses. USP15 plays a central role in the regulation of TGF-β signaling by deubiquitinating and stabilizing SMAD proteins that are important for signal transduction. In addition to TGF-β modulation USP15 also participates in endocytic trafficking by associating with the ESCRT complex impacting receptor degradation and cell signaling.
- Pathways
The function of USP15 is critical in integrating cellular signaling networks such as the TGF-β and NF-kB pathways. Within the TGF-β pathway USP15 deubiquitinates receptor-regulated SMADs maintaining their stability and enhancing signal transduction. In the NF-kB pathway USP15 influences the activation by regulating the degradation of IκBα an inhibitor of NF-kB which indicates its role in immune response and inflammation. Through these pathways USP15 interacts with proteins like SMAD2/3 in TGF-β signaling and IκBα in NF-kB regulation.
- Associated diseases and disorders
USP15 has associations with cancer and neurodegenerative diseases. In cancer altered USP15 activity leads to dysregulation of TGF-β signaling influencing tumor progression and metastasis. The enzyme interacts with proteins such as MDM2 in neurodegenerative disorders affecting p53 stability and neuronal cell survival. This suggests a role in processes like apoptosis and DNA repair critical in the onset and progression of neurodegenerative conditions. But current research continues aiming for better understanding of USP15's connections with these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-USP15 antibody (ab97533)
7.5% SDS PAGE
All lanes: Western blot - Anti-USP15 antibody (ab97533) at 1/1000 dilution
All lanes: MOLT4 whole cell lysate at 30 µg
Predicted band size: 112 kDa
Immunocytochemistry/ Immunofluorescence - Anti-USP15 antibody (ab97533)
Immunofluorescence analysis of paraformaldehyde-fixed A431, using USP15(GTX104644) antibody at 1:200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (ab97533)
Immunohistochemical analysis of USP15 in paraffin embedded N87 xenograft, using ab97533 at a 1/100 dilution.
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