JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB269961

Anti-USP16 antibody [EPR20914-47] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal USP16 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase 16, Deubiquitinating enzyme 16, Ubiquitin thioesterase 16, Ubiquitin-specific-processing protease 16, Usp16

1 Images
Western blot - Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (AB269961)
  • WB

Lab

Western blot - Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (AB269961)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 26323689).

Exposure time : Lanes 1-3 : 3 minutes Lane 4 : 48 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab236628).

All lanes:

Western blot - Anti-USP16 antibody [EPR20914-47] (<a href='/en-us/products/primary-antibodies/usp16-antibody-epr20914-47-ab236628'>ab236628</a>) at 1/1000 dilution

Lane 1:

Rat testis tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 110 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20914-47

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
style
left-column

Product details

ab269961 is the carrier-free version of ab236628.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP16 also known as Ubiquitin Specific Peptidase 16 functions as a deubiquitinating enzyme that regulates protein degradation by cleaving ubiquitin from ubiquitin-conjugated protein substrates. It has a molecular mass of approximately 97 kDa. USP16 is expressed in various tissues with high levels in the brain and heart. It has an integral role in maintaining protein homeostasis by regulating degradation pathways necessary for cellular function.
Biological function summary

The enzymatic activity of USP16 removes ubiquitin moieties contributing to the stabilization of target proteins. It participates in several cellular processes including cell cycle regulation DNA damage response and chromatin remodeling. USP16 is associated with the Polycomb group (PcG) complex which plays a role in transcriptional repression. This involvement in epigenetic regulation highlights its importance in gene expression and cellular differentiation.

Pathways

USP16 is significant in the Wnt signaling pathway and the Hedgehog signaling pathway. In the Wnt pathway USP16 regulates beta-catenin degradation influencing cell proliferation and differentiation. Through the Hedgehog pathway USP16 impacts the stability of GLI transcription factors affecting developmental processes and tissue patterning. USP16 interacts with proteins like Axin and APC in the Wnt pathway illustrating its role in complex regulatory networks.

Researchers have found connections between USP16 dysregulation and cancer particularly leukemia and neurodegenerative diseases like Alzheimer's disease. In cancer USP16 overexpression can lead to increased cell proliferation through pathways involving proteins such as MYC and Cyclin D1. Related to neurodegenerative disorders USP16's interaction with proteins like amyloid precursor protein (APP) contributes to pathologies like Alzheimer's where proteostasis and ubiquitination play critical roles. Understanding USP16's functions and connections can provide insights into therapeutic targets for these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator (PubMed : 24034696). Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis (PubMed : 24034696). In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes (PubMed : 24034696). Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. Also deubiquitinates non-histone proteins, such as ribosomal protein RPS27A : deubiquitination of monoubiquitinated RPS27A promotes maturation of the 40S ribosomal subunit. Also mediates deubiquitination of tektin proteins (TEKT1, TEKT2, TEK3, TEKT4 and TEKT5), promoting their stability (PubMed : 36206347).
See full target information Usp16
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB124966

Anti-PCYOX1 antibody [EPR5611]

RabMAb|Recombinant

Western blot - Anti-PCYOX1 antibody [EPR5611] (AB124966)

  • 0
  • 0
Assay Kits

AB65393

LDH Assay Kit (Cytotoxicity)

Biochemical assay - LDH Assay Kit (Cytotoxicity) (AB65393)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB307601

Anti-xCT antibody [EPR27115-64]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-xCT antibody [EPR27115-64] (AB307601)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com