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AB250993

Anti-USP19 antibody [EPR14816] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal USP19 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0891, ZMYND9, USP19, Ubiquitin carboxyl-terminal hydrolase 19, Deubiquitinating enzyme 19, Ubiquitin thioesterase 19, Ubiquitin-specific-processing protease 19, Zinc finger MYND domain-containing protein 9

4 Images
Flow Cytometry (Intracellular) - Anti-USP19 antibody [EPR14816] - BSA and Azide free (AB250993)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-USP19 antibody [EPR14816] - BSA and Azide free (AB250993)

This data was developed using ab189518, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of Jurkat cells (left histogram) labeling USP19 with ab189518 at 1/250 dilution compared to negative control cells (right histogram). Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.

Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (AB250993)
  • WB

Supplier Data

Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (AB250993)

This data was developed using ab189518, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-USP19 antibody [EPR14816] (<a href='/en-us/products/primary-antibodies/usp19-antibody-epr14816-ab189518'>ab189518</a>) at 1/10000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

293T lysate at 10 µg

Lane 3:

HeLa lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 146 kDa

true

Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (AB250993)
  • WB

Lab

Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (AB250993)

This data was developed using ab189518, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (40 μg)

Lane 2 : USP19 knockout HAP1 cell lysate (40 μg)

Lane 3 : Jurkat cell lysate (40 μg)

Lane 4 : HeLa cell lysate (40 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab189518 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab189518 was shown to recognize USP19 when USP19 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP19 knockout samples were subjected to SDS-PAGE. ab189518 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-USP19 antibody [EPR14816] (<a href='/en-us/products/primary-antibodies/usp19-antibody-epr14816-ab189518'>ab189518</a>)

Predicted band size: 146 kDa

false

Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (AB250993)
  • WB

Supplier Data

Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (AB250993)

This data was developed using ab189518, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-USP19 antibody [EPR14816] (<a href='/en-us/products/primary-antibodies/usp19-antibody-epr14816-ab189518'>ab189518</a>) at 1/5000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 146 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14816

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB187156

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Primary Antibodies

AB129002

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Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - Loading Control (AB129002)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250993 is the carrier-free version of ab189518.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP19 also known as Ubiquitin Specific Peptidase 19 is an enzyme with deubiquitinating activity. It belongs to the family of cysteine proteases and has a molecular mass of approximately 150 kDa. This protein's primary function involves the removal of ubiquitin from specific protein substrates regulating their degradation and maintaining protein homeostasis. USP19 is expressed in multiple tissues including skeletal muscle heart and brain indicating its widespread physiological roles.
Biological function summary

The regulation of protein turnover and cellular stress responses involves USP19. As a standalone enzyme it participates independently rather than as part of a larger protein complex. By deubiquitinating target proteins USP19 influences their stability and function affecting a variety of cellular processes such as protein synthesis cell cycle progression and stress response through modulation of ubiquitin chains on substrates.

Pathways

The modulation of protein degradative pathways involves USP19 specifically impacting the ubiquitin-proteasome system (UPS) and chaperone-mediated autophagy (CMA). It interacts with key proteins in these pathways such as ubiquitin-conjugating enzymes and proteasomal subunits to maintain cellular homeostasis. By regulating pathways like UPS USP19 plays a role in controlling protein quality and preventing aggregation which is critical for cell function.

The dysregulation of protein degradation mechanisms implicates USP19 in conditions such as muscle atrophy and neurodegenerative diseases. Abnormal activity of USP19 associates with disorders like Parkinson’s disease where it may interact with proteins such as Parkin affecting the ubiquitination balance. Studies on USP19 continue to explore its potential as a therapeutic target for treating these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinating enzyme that regulates the degradation of various proteins by removing ubiquitin moieties, thereby preventing their proteasomal degradation. Stabilizes RNF123, which promotes CDKN1B degradation and contributes to cell proliferation (By similarity). Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates (PubMed : 19465887, PubMed : 24356957). Mechanistically, deubiquitinates and thereby stabilizes several E3 ligases involved in the ERAD pathway including SYVN1 or MARCHF6 (PubMed : 24356957). Regulates the stability of other E3 ligases including BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing their ubiquitination (PubMed : 21849505). Required for cells to mount an appropriate response to hypoxia by rescuing HIF1A from degradation in a non-catalytic manner and by mediating the deubiquitination of FUNDC1 (PubMed : 22128162, PubMed : 33978709). Attenuates mitochondrial damage and ferroptosis by targeting and stabilizing NADPH oxidase 4/NOX4 (PubMed : 38943386). Negatively regulates TNF- and IL-1beta-triggered NF-kappa-B activation by hydrolyzing 'Lys-27'- and 'Lys-63'-linked polyubiquitin chains from MAP3K7 (PubMed : 31127032). Modulates also the protein level and aggregation of polyQ-expanded huntingtin/HTT through HSP90AA1 (PubMed : 33094816).
See full target information USP19
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 14: PubMed37239471

2023

Differential Regulation of POC5 by ERα in Human Normal and Scoliotic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amani Hassan,Edward T Bagu,Shunmoogum A Patten,Sirinart Molidperee,Stefan Parent,Soraya Barchi,Isabelle Villemure,André Tremblay,Florina Moldovan
View all publications
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Product promise

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