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AB248274

Anti-USP24 antibody [EPR7060] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal USP24 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA1057, USP24, Ubiquitin carboxyl-terminal hydrolase 24, Deubiquitinating enzyme 24, Ubiquitin thioesterase 24, Ubiquitin-specific-processing protease 24

2 Images
Western blot - Anti-USP24 antibody [EPR7060] - BSA and Azide free (AB248274)
  • WB

Unknown

Western blot - Anti-USP24 antibody [EPR7060] - BSA and Azide free (AB248274)

This data was developed using ab129064, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-USP24 antibody [EPR7060] (<a href='/en-us/products/primary-antibodies/usp24-antibody-epr7060-ab129064'>ab129064</a>) at 1/10000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 294 kDa

Observed band size: 290 kDa

false

OI-RD Scanning - Anti-USP24 antibody [EPR7060] - BSA and Azide free (AB248274)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-USP24 antibody [EPR7060] - BSA and Azide free (AB248274)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7060

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab248274 is the carrier-free version of ab129064.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP24 also known as Ubiquitin Specific Peptidase 24 is a deubiquitinating enzyme with a molecular mass of approximately 291 kDa. This protein plays a role in the removal of ubiquitin from specific protein substrates which regulates their degradation in the proteasome. It involves the cleavage of the ubiquitin moieties and alters the stability or activity of proteins. USP24 shows expression in several tissues with notable presence in the brain and testis. The specificity of USP24 allows it to finely adjust cellular protein turnover affecting both normal cellular function and stress responses.
Biological function summary

The regulation of protein ubiquitination/deubiquitination is fundamental in maintaining cellular homeostasis. USP24 acts within this regulatory landscape to control pathways involved in cell cycle progression and DNA damage response. It can interact with other proteins and may form complexes under certain cellular conditions affecting its activity and specificity. These complexes can further indicate how USP24’s enzymatic action coordinates larger cellular processes modulating critical phases of a cell's life cycle and response to external damage.

Pathways

Proteins like USP24 play decisive roles in the ubiquitin-proteasome pathway. This pathway is essential for controlling protein degradation ensuring that excess damaged or misfolded proteins are effectively processed. USP24 specifically interacts with and stabilizes proteins like p53 which is fundamental in DNA repair pathways. It links the ubiquitin-proteasome system to critical cellular defense mechanisms against DNA damage helping maintain genomic integrity. There is also involvement with pathways related to neuronal survival suggesting a broader functional landscape for USP24 beyond cellular maintenance.

Disruptions in USP24 activity have been implicated in various neurodegenerative diseases including Alzheimer's disease. Altered deubiquitination activity may contribute to the accumulation of pathogenic proteins exacerbating neuronal damage. Furthermore USP24 has connections with cancers particularly through its stabilization of tumor suppressor proteins like p53. Aberrant regulation of USP24 could result in ineffective tumor suppression leading to unchecked cellular proliferation. The relationship of USP24 with both Alzheimer's disease and cancer shows its importance in maintaining cellular and organismal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-specific protease that regulates cell survival in various contexts through modulating the protein stability of some of its substrates including DDB2, MCL1 or TP53. Plays a positive role on ferritinophagy where ferritin is degraded in lysosomes and releases free iron.
See full target information USP24
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 10:e13735 PubMed35891648

2022

Identification and validation of immune related core transcription factors in NAFLD.

Applications

Unspecified application

Species

Unspecified reactive species

Minbo Zhang,Yu Zhang,Xiaoxiao Jiao,Linying Lai,Yiting Qian,Bo Sun,Wenzhuo Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com