AB191703

Anti-USP25 antibody

Name: Anti-USP25 antibody (ab191703) | Abcam
Brand: Abcam Limited
SKU: AB191703

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal USP25 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human USP25.

View Alternative Names

USP21, USP25, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25

1 Images

Supplier Data

Western blot - Anti-USP25 antibody (AB191703)

All lanes:

Western blot - Anti-USP25 antibody (ab191703) at 1 µg/mL

Lane 1:

mouse brain tissue lysate at 15 µg

Lane 2:

mouse brain tissue lysate at 15 µg with blocking peptide

Predicted band size: 122 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human USP25. The exact immunogen used to generate this antibody is proprietary information.

Q9UHP3

Specificity

Multiple isoforms of USP25 are known to exist.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP25 also known as Ubiquitin Specific Peptidase 25 is a deubiquitinating enzyme that removes ubiquitin moieties from polyubiquitinated proteins. It has a molecular mass of approximately 120 kDa. This protein is expressed in various tissues with notable expression in the brain kidney and thymus. USP25 plays an essential role in regulating the balance between protein ubiquitination and deubiquitination which is critical for maintaining cellular protein homeostasis.

Biological function summary

USP25 contributes to the modulation of several key proteins by rescuing them from proteasomal degradation through deubiquitination. This protein is involved in multiple cellular processes such as signal transduction and the immune response. USP25 is part of the endoplasmic reticulum-associated degradation (ERAD) complex where it functions to recycle ubiquitin molecules from substrates destined for degradation. By doing so USP25 influences the stability and localization of various proteins impacting cell growth and survival.

Pathways

USP25 is involved in the ubiquitin-proteasome system (UPS) and the Wnt signaling pathway. In the UPS USP25 interacts with proteins like LRP6 helping regulate the amount of ß-catenin thereby influencing Wnt signaling activity. This interaction plays a role in controlling cell proliferation and differentiation. USP25 also associates with other deubiquitinating enzymes reflecting its role in multiple regulatory pathways essential for cellular function.

Research links USP25 to inflammatory diseases and certain cancers. Its role in cancer is as a regulator of tumor-suppressor proteins and oncogenes such as those involving p53 pathways. Inflammatory conditions like rheumatoid arthritis show altered USP25 expression patterns where it interacts with proteins like TNF-alpha receptor-associated factors (TRAFs) impacting inflammatory signaling cascades. Studies continue to explore USP25's potential as a therapeutic target in these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions in various biological processes including inflammation, immune response (PubMed : 29518389, PubMed : 37683630). Modulates the Wnt/beta-catenin pathway by deubiquitinating and stabilizing tankyrases TNKS1 and TNKS2 (PubMed : 28619731, PubMed : 30926243). Regulates KEAP1-NRF2 axis in the defense against oxidative assaults by deubiquitinating KEAP1 and protecting it from degradation leading to degradation of the NRF2 transcription factor that is responsible for mounting an anti-oxidation gene expression program (PubMed : 37339955). Positively regulates RNA virus-induced innate signaling by interacting with and deubiquitinating ERLIN1 and ERLIN2 (PubMed : 37683630). In turn, restricts virus production by regulating cholesterol biosynthetic flux (PubMed : 37683630). Acts as a negative regulator of interleukin-17-mediated signaling and inflammation through the removal of 'Lys-63'-linked ubiquitination of TRAF5 and TRAF6 (PubMed : 23042150). Prevents the ubiquitination and degradation of TRAF3 to reduce the phosphorylation levels of JNK and P38, the secretion of IL-1B and to induce endotoxin tolerance (PubMed : 30579117).. The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

See full target information USP25

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 20:115 PubMed37183260

2023

Novel CH25H and OASL microglia subclusters play distinct roles in cerebral ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yueman Zhang,Yunlu Guo,Ruqi Li,Tingting Huang,Yan Li,Wanqin Xie,Chen Chen,Weijie Chen,Jieqing Wan,Weifeng Yu,Peiying Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP25 antibody

Western blot - Anti-USP25 antibody (AB191703)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise