AB187156

Anti-USP25 antibody [EPR15019]

20ul selling size
RabMAb
Recombinant
What is this?

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(31 Publications)

Rabbit Recombinant Monoclonal USP25 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 31 publications.

View Alternative Names

USP21, USP25, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25

3 Images

Flow Cytometry (Intracellular) - Anti-USP25 antibody [EPR15019] (AB187156)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-USP25 antibody [EPR15019] (AB187156)

Intracellular Flow Cytometry analysis of USP25 expression in 2% paraformaldehyde-fixed A673 cells using ab187156 at 1/60 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody.

Supplier Data

Western blot - Anti-USP25 antibody [EPR15019] (AB187156)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-USP25 antibody [EPR15019] (ab187156) at 1/10000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

A673 cell lysate at 20 µg

Lane 3:

BxPC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 122 kDa

Observed band size: 122 kDa

false

Supplier Data

Western blot - Anti-USP25 antibody [EPR15019] (AB187156)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-USP25 antibody [EPR15019] (ab187156) at 1/1000 dilution

Lane 1:

Raw264.7 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 122 kDa

false

Carrier free

Anti-USP25 antibody [EPR15019] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15019

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP25 also known as Ubiquitin Specific Peptidase 25 is a deubiquitinating enzyme that removes ubiquitin moieties from polyubiquitinated proteins. It has a molecular mass of approximately 120 kDa. This protein is expressed in various tissues with notable expression in the brain kidney and thymus. USP25 plays an essential role in regulating the balance between protein ubiquitination and deubiquitination which is critical for maintaining cellular protein homeostasis.

Biological function summary

USP25 contributes to the modulation of several key proteins by rescuing them from proteasomal degradation through deubiquitination. This protein is involved in multiple cellular processes such as signal transduction and the immune response. USP25 is part of the endoplasmic reticulum-associated degradation (ERAD) complex where it functions to recycle ubiquitin molecules from substrates destined for degradation. By doing so USP25 influences the stability and localization of various proteins impacting cell growth and survival.

Pathways

USP25 is involved in the ubiquitin-proteasome system (UPS) and the Wnt signaling pathway. In the UPS USP25 interacts with proteins like LRP6 helping regulate the amount of ß-catenin thereby influencing Wnt signaling activity. This interaction plays a role in controlling cell proliferation and differentiation. USP25 also associates with other deubiquitinating enzymes reflecting its role in multiple regulatory pathways essential for cellular function.

Research links USP25 to inflammatory diseases and certain cancers. Its role in cancer is as a regulator of tumor-suppressor proteins and oncogenes such as those involving p53 pathways. Inflammatory conditions like rheumatoid arthritis show altered USP25 expression patterns where it interacts with proteins like TNF-alpha receptor-associated factors (TRAFs) impacting inflammatory signaling cascades. Studies continue to explore USP25's potential as a therapeutic target in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions in various biological processes including inflammation and immune response (PubMed : 29518389, PubMed : 37683630). Modulates the Wnt/beta-catenin pathway by deubiquitinating and stabilizing tankyrases TNKS1 and TNKS2 (PubMed : 28619731, PubMed : 30926243, PubMed : 38875478). Regulates KEAP1-NRF2 axis in the defense against oxidative assaults by deubiquitinating KEAP1 and protecting it from degradation leading to degradation of the NRF2 transcription factor that is responsible for mounting an anti-oxidation gene expression program (PubMed : 37339955). Positively regulates RNA virus-induced innate signaling by interacting with and deubiquitinating ERLIN1 and ERLIN2 (PubMed : 37683630). In turn, restricts virus production by regulating cholesterol biosynthetic flux (PubMed : 37683630). Acts as a negative regulator of interleukin-17-mediated signaling and inflammation through the removal of 'Lys-63'-linked ubiquitination of TRAF5 and TRAF6 (PubMed : 23042150). Prevents the ubiquitination and degradation of TRAF3 to reduce the phosphorylation levels of JNK and P38, the secretion of IL-1B and to induce endotoxin tolerance (PubMed : 30579117).. The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

See full target information USP25

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:575 PubMed40830790

2025

Engineered MEVs for photoreceptor-targeted delivery of USP25 to alleviate diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxiang Sun,Shenyuan Chen,Xiaoyuan Qi,Yuntong Sun,Zhengmei Jiang,Jie Chang,Yongjun Ma,Jin Huang,Benshuai You,Fengtian Sun

Clinical and translational medicine 15:e70243 PubMed39985261

2025

Ubiquitin-specific protease 25 improves myocardial ischemia-reperfusion injury by deubiquitinating NLRP3 and negatively regulating NLRP3 inflammasome activity in cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Bozhi Ye,Diyun Xu,Lingfeng Zhong,Yi Wang,Wei Wang,Haowen Xu,Xue Han,Julian Min,Gaojun Wu,Wenhai Huang,Guang Liang

International journal of biological sciences 21:475-489 PubMed39781451

2025

USP25 stabilizes STAT6 to promote IL-4-induced macrophage M2 polarization and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yahan Xu,Jing Liu,Jingzeng Wang,Jiayao Wang,Peixiang Lan,Tao Wang

EMBO molecular medicine 16:2856-2881 PubMed39394468

2024

Targeting USP11 regulation by a novel lithium-organic coordination compound improves neuropathologies and cognitive functions in Alzheimer transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Guo,Chuanbin Cai,Bingjie Zhang,Bo Tan,Qinmin Tang,Zhifeng Lei,Xiaolan Qi,Jiang Chen,Xiaojiang Zheng,Dan Zi,Song Li,Jun Tan

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403485 PubMed38803048

2024

USP25 Elevates SHLD2-Mediated DNA Double-Strand Break Repair and Regulates Chemoresponse in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhui Li,Lei Li,Xinshu Wang,Fei Zhao,Yuntong Yang,Yujuan Zhou,Jiyuan Zhang,Li Wang,Zeshan Jiang,Yuanyuan Zhang,Yuping Chen,Chenming Wu,Ke Li,Tingting Zhang,Ping Wang,Zhiyong Mao,Weiguo Zhu,Xingzhi Xu,Shikang Liang,Zhenkun Lou,Jian Yuan

Inflammation 47:1520-1535 PubMed38436811

2024

A New Modulator of Neuroinflammation in Diabetic Retinopathy: USP25.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Hu,Xue Zhang,Hongsong Peng,Jitian Guan,Zhangxin Huang,Bo Jiang,Dawei Sun

Frontiers in immunology 14:1252827 PubMed37841261

2023

Modified activities of macrophages' deubiquitinating enzymes after infection.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Vozandychova,Pavel Rehulka,Kamil Hercik,Petra Spidlova,Pavla Pavlik,Jaroslav Hanus,Romana Hadravova,Jiri Stulik

Developmental cell 58:2495-2509.e6 PubMed37683630

2023

Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wen Teo,Ho Him Wong,Tiaan Heunis,Viktoriya Stancheva,Asmaa Hachim,Huibin Lv,Lewis Siu,Julian Ho,Yun Lan,Chris Ka Pun Mok,Rachel Ulferts,Sumana Sanyal

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2301641 PubMed37587766

2023

USP25 Inhibits Neuroinflammatory Responses After Cerebral Ischemic Stroke by Deubiquitinating TAB2.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongding Li,Baohua Liu,Kate Lykke Lambertsen,Bettina Hjelm Clausen,Zhenhu Zhu,Xue Du,Yanqi Xu,Frantz Rom Poulsen,Bo Halle,Christian Bonde,Meng Chen,Xue Wang,Dirk Schlüter,Jingyong Huang,Ari Waisman,Weihong Song,Xu Wang

Biochimica et biophysica acta. Molecular basis of disease 1869:166713 PubMed37059312

2023

USP25 inhibits renal fibrosis by regulating TGFβ-SMAD signaling pathway in Ang II-induced hypertensive mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhao,Xi Chen,Yimin Lin,Zhongding Li,Xian Su,Shijie Fan,Yanghao Chen,Xu Wang,Guang Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP25 antibody [EPR15019]

Flow Cytometry (Intracellular) - Anti-USP25 antibody [EPR15019] (AB187156)

Western blot - Anti-USP25 antibody [EPR15019] (AB187156)

Western blot - Anti-USP25 antibody [EPR15019] (AB187156)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (31)

Product promise