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AB188240

Anti-USP28 antibody

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(3 Publications)

Rabbit Polyclonal USP28 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP28 aa 1-400.

View Alternative Names

KIAA1515, USP28, Ubiquitin carboxyl-terminal hydrolase 28, Deubiquitinating enzyme 28, Ubiquitin thioesterase 28, Ubiquitin-specific-processing protease 28

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-USP28 antibody (AB188240)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP28 antibody (AB188240)

Immunofluorescent analysis of 3% PFA-fixed U2OS cells transfected with either a control si (left) or USP28 si (right) labeling USP28 with ab188240 at 1/2000 dilution.

Immunoprecipitation - Anti-USP28 antibody (AB188240)
  • IP

Supplier Data

Immunoprecipitation - Anti-USP28 antibody (AB188240)

Western blot analysis (using ab188240) of immunoprecipitates from U2OS cell extracts

Lane 1 : Input

Lane 2 : immunoprecipitate with control serum+protein A sepharose.

Lane 3 : immunoprecipitate with ab188240 + protein A sepharose.

All lanes:

Immunoprecipitation - Anti-USP28 antibody (ab188240)

Predicted band size: 122 kDa

false

Western blot - Anti-USP28 antibody (AB188240)
  • WB

Supplier Data

Western blot - Anti-USP28 antibody (AB188240)

All lanes:

Western blot - Anti-USP28 antibody (ab188240) at 1/2000 dilution

Lane 1:

Lysate from U2OS cells transfected with a control si at 40 µg

Lane 2:

Lysate from U2OS cells transfected with a USP28 si at 40 µg

Lane 3:

NIH 3T3 cell lysate at 40 µg

Lane 4:

INS-1 cell lysate at 40 µg

Predicted band size: 122 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human USP28 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

Q96RU2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP28 also known as Ubiquitin Specific Peptidase 28 is a deubiquitinating enzyme responsible for removing ubiquitin from protein substrates. It belongs to the cysteine protease family and has a molecular mass of approximately 120 kDa. USP28 is mainly expressed in the nucleus and cytoplasm of cells and appears in various tissues including the lung liver and gastrointestinal tract. It plays a role in regulating protein stability by counteracting ubiquitination an important process for protein degradation.
Biological function summary

This deubiquitinase regulates several cellular processes such as DNA damage response cell cycle progression and apoptosis. USP28 influences protein stability by directly associating with components of the c-MYC signaling pathway. It is known to interact with and stabilize Fbw7 which is part of the ubiquitin ligase complex thereby affecting the turnover of proteins like c-MYC and cyclin E. Through these interactions USP28 helps maintain normal levels of these important regulators for healthy cellular functions.

Pathways

The enzyme plays an important part in the DNA damage response and MAPK signaling pathways. These pathways ensure proper genomic integrity and cell proliferation. USP28 enhances the stability of the key regulatory protein c-MYC in the p53 pathway emphasizing its role in cell cycle regulation. Its interaction with other proteins like Fbw7 and c-MYC allows it to modulate the rate of cell growth and division indicating its importance in controlled cellular pathways.

USP28 has connections to cancer and neurodegenerative diseases. Its interactions with c-MYC are implicated in tumorigenesis particularly in colorectal and breast cancers due to the stabilization of oncogenic proteins that promote uncontrollable cell proliferation. Additionally USP28 might contribute to neurodegenerative disorders by affecting protein aggregates commonly seen in such diseases although studies are less established in this area. The enzyme's strong connection with proteins like Fbw7 and c-MYC highlights its relevance in these disease situations making it a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome : acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed : 24623306).
See full target information USP28
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:831 PubMed40993725

2025

USP28 participates in high glucose-mediated endothelial dysfunction via deubiquitinating SIRT1 protein in diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Liu,Jin Zhang,Jichang Bai,Kuanzhi Liu

Nature communications 14:686 PubMed36754960

2023

Accelerating inhibitor discovery for deubiquitinating enzymes.

Applications

WB

Species

Human

Wai Cheung Chan,Xiaoxi Liu,Robert S Magin,Nicholas M Girardi,Scott B Ficarro,Wanyi Hu,Maria I Tarazona Guzman,Cara A Starnbach,Alejandra Felix,Guillaume Adelmant,Anthony C Varca,Bin Hu,Ariana S Bratt,Ethan DaSilva,Nathan J Schauer,Isabella Jaen Maisonet,Emma K Dolen,Anthony X Ayala,Jarrod A Marto,Sara J Buhrlage

Cancer letters 448:52-60 PubMed30763716

2019

USP26 promotes esophageal squamous cell carcinoma metastasis through stabilizing Snail.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Li,Honghong Zhou,Rui Zhu,Zhihua Liu
View all publications
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Product promise

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