Rabbit Polyclonal USP3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human USP3.
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Publications
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- Nature cell biology 23:1287-12982021XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarek Adamowicz,Richard Hailstone,Annie A Demin,Emilia Komulainen,Hana Hanzlikova,Jan Brazina,Amit Gautam,Sophie E Wells,Keith W CaldecottPubMed 34811483
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human USP3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6I4
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/20000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rhesus monkey, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rhesus monkey, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. In response to DNA damage, deubiquitinates monoubiquitinated target proteins such as histone H2A and H2AX and thereby counteracts RNF168- and RNF8-mediated ubiquitination. In turn, participates in the recruitment of DNA damage repair factors to DNA break sites (PubMed:24196443). Required for proper progression through S phase and subsequent mitotic entry (PubMed:17980597). Acts as a positive regulator of TP53 by deubiquitinating and stabilizing it to promote normal cell proliferation and transformation (PubMed:28807825). Participates in establishing tolerance innate immune memory through non-transcriptional feedback. Mechanistically, negatively regulates TLR-induced NF-kappa-B signaling by targeting and removing the 'Lys-63'-linked polyubiquitin chains on MYD88 (PubMed:37971847). Negatively regulates the activation of type I interferon signaling by mediating 'Lys-63'-linked polyubiquitin chains on RIGI and IFIH1 (PubMed:24366338). Deubiquitinates also ASC/PYCARD, the central adapter mediating the assembly and activation of most inflammasomes, and thereby promotes inflammasome activation (PubMed:36050480).
Alternative names
Ubiquitin carboxyl-terminal hydrolase 3, Deubiquitinating enzyme 3, Ubiquitin thioesterase 3, Ubiquitin-specific-processing protease 3, USP3
Recommended products
Rabbit Polyclonal USP3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human USP3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human USP3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6I4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP3 also known as Ubiquitin Specific Peptidase 3 is an enzyme that functions as a deubiquitinating enzyme (DUB). This protein plays a role in removing ubiquitin moieties from polyubiquitinated protein substrates. By regulating ubiquitination USP3 controls protein degradation. The full-length human USP3 protein has a molecular mass of approximately 109 kDa. Scientists have found USP3 expression in various tissues including the liver lungs and kidneys indicating its widespread biological significance.
- Biological function summary
USP3 influences the regulation of the cell cycle and genome maintenance. It does not function alone; USP3 can associate with other proteins to form complexes that modulate these intracellular processes. Its activity in deubiquitination contributes to chromatin remodeling by stabilizing or activating proteins involved in cellular division and DNA repair. Therefore USP3 plays a role in maintaining cellular integrity and preventing genomic instability.
- Pathways
Deubiquitination events involving USP3 impact key regulatory circuits like the DNA damage response and cell cycle checkpoints. Within these pathways USP3 collaborates with proteins such as H2A and H2AX where it maintains their stability by deubiquitinating them. By influencing these pathways USP3 ensures the proper execution of cell cycle transitions and the DNA damage response preventing errors during replication and division.
- Associated diseases and disorders
USP3 has implications in both cancer and neurodegenerative diseases. Its dysregulation contributes to abnormal cell proliferation and defective DNA repair mechanisms which are hallmarks of various cancers including breast cancer. USP3 is also connected to Rett syndrome where it affects the stability of proteins like MECP2 involved in neuronal function. Understanding USP3's role in these diseases highlights its potential as a therapeutic target for improving treatment strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-USP3 antibody (ab229348)
K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for USP3 (green) using ab229348 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
Western blot - Anti-USP3 antibody (ab229348)
7.5 % SDS-PAGE gel.
All lanes: Western blot - Anti-USP3 antibody (ab229348) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Predicted band size: 59 kDa
Western blot - Anti-USP3 antibody (ab229348)
7.5 % SDS-PAGE gel.
All lanes: Western blot - Anti-USP3 antibody (ab229348) at 1/20000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: Human USP3-transfected HEK-293T whole cell lysate at 30 µg
Predicted band size: 59 kDa
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