AB229348

Anti-USP3 antibody

Name: Anti-USP3 antibody (ab229348) | Abcam
Brand: Abcam Limited
SKU: AB229348
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal USP3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP3.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase 3, Deubiquitinating enzyme 3, Ubiquitin thioesterase 3, Ubiquitin-specific-processing protease 3, USP3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-USP3 antibody (AB229348)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP3 antibody (AB229348)

K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for USP3 (green) using ab229348 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-USP3 antibody (AB229348)

7.5 % SDS-PAGE gel.

All lanes:

Western blot - Anti-USP3 antibody (ab229348) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 59 kDa

false

Supplier Data

Western blot - Anti-USP3 antibody (AB229348)

7.5 % SDS-PAGE gel.

All lanes:

Western blot - Anti-USP3 antibody (ab229348) at 1/20000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

Human USP3-transfected HEK-293T whole cell lysate at 30 µg

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human USP3. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6I4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP3 also known as Ubiquitin Specific Peptidase 3 is an enzyme that functions as a deubiquitinating enzyme (DUB). This protein plays a role in removing ubiquitin moieties from polyubiquitinated protein substrates. By regulating ubiquitination USP3 controls protein degradation. The full-length human USP3 protein has a molecular mass of approximately 109 kDa. Scientists have found USP3 expression in various tissues including the liver lungs and kidneys indicating its widespread biological significance.

Biological function summary

USP3 influences the regulation of the cell cycle and genome maintenance. It does not function alone; USP3 can associate with other proteins to form complexes that modulate these intracellular processes. Its activity in deubiquitination contributes to chromatin remodeling by stabilizing or activating proteins involved in cellular division and DNA repair. Therefore USP3 plays a role in maintaining cellular integrity and preventing genomic instability.

Pathways

Deubiquitination events involving USP3 impact key regulatory circuits like the DNA damage response and cell cycle checkpoints. Within these pathways USP3 collaborates with proteins such as H2A and H2AX where it maintains their stability by deubiquitinating them. By influencing these pathways USP3 ensures the proper execution of cell cycle transitions and the DNA damage response preventing errors during replication and division.

USP3 has implications in both cancer and neurodegenerative diseases. Its dysregulation contributes to abnormal cell proliferation and defective DNA repair mechanisms which are hallmarks of various cancers including breast cancer. USP3 is also connected to Rett syndrome where it affects the stability of proteins like MECP2 involved in neuronal function. Understanding USP3's role in these diseases highlights its potential as a therapeutic target for improving treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. In response to DNA damage, deubiquitinates monoubiquitinated target proteins such as histone H2A and H2AX and thereby counteracts RNF168- and RNF8-mediated ubiquitination. In turn, participates in the recruitment of DNA damage repair factors to DNA break sites (PubMed : 24196443). Required for proper progression through S phase and subsequent mitotic entry (PubMed : 17980597). Acts as a positive regulator of TP53 by deubiquitinating and stabilizing it to promote normal cell proliferation and transformation (PubMed : 28807825). Participates in establishing tolerance innate immune memory through non-transcriptional feedback. Mechanistically, negatively regulates TLR-induced NF-kappa-B signaling by targeting and removing the 'Lys-63'-linked polyubiquitin chains on MYD88 (PubMed : 37971847). Negatively regulates the activation of type I interferon signaling by mediating 'Lys-63'-linked polyubiquitin chains on RIGI and IFIH1 (PubMed : 24366338). Deubiquitinates also ASC/PYCARD, the central adapter mediating the assembly and activation of most inflammasomes, and thereby promotes inflammasome activation (PubMed : 36050480).

See full target information USP3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:228 PubMed39948151

2025

The proteomic landscape of stool-derived extracellular vesicles in patients with pre-cancerous lesions and colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emmalee J Northrop-Albrecht,Yohan Kim,William R Taylor,Shounak Majumder,John B Kisiel,Fabrice Lucien

Nature communications 16:1313 PubMed39900921

2025

USP5 stabilizes YTHDF1 to control cancer immune surveillance through mTORC1-mediated phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Na Shao,Lei Xi,Yangfan Lv,Muhammad Idris,Lin Zhang,Ya Cao,Jingyi Xiang,Xi Xu,Belinda X Ong,Qiongyi Zhang,Xu Peng,Xiaoyan Yue,Feng Xu,Chungang Liu

RNA biology 19:482-495 PubMed35373703

2022

A positive feedback loop between LINC01605 and NF-κB pathway promotes tumor growth in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weiguo Zhao,Ling Xin,Lei Tang,Yunjing Li,Xueqin Li,Ruifeng Liu

Nature cell biology 23:1287-1298 PubMed34811483

2021

XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Marek Adamowicz,Richard Hailstone,Annie A Demin,Emilia Komulainen,Hana Hanzlikova,Jan Brazina,Amit Gautam,Sophie E Wells,Keith W Caldecott

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-USP3 antibody (AB229348)

Western blot - Anti-USP3 antibody (AB229348)

Western blot - Anti-USP3 antibody (AB229348)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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