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Rabbit Recombinant Monoclonal USP30 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-USP30 antibody [EPR27024-81] (AB314749), expandable thumbnail
  • Western blot - Anti-USP30 antibody [EPR27024-81] (AB314749), expandable thumbnail
  • Western blot - Anti-USP30 antibody [EPR27024-81] (AB314749), expandable thumbnail
  • Western blot - Anti-USP30 antibody [EPR27024-81] (AB314749), expandable thumbnail
  • Western blot - Anti-USP30 antibody [EPR27024-81] (AB314749), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt (Intra)IHC-Fr
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951). Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling (PubMed:25621951). Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (PubMed:25527291). Acts as a negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity).

Alternative names

Ubiquitin carboxyl-terminal hydrolase 30, Deubiquitinating enzyme 30, Ubiquitin thioesterase 30, Ubiquitin-specific-processing protease 30, Ub-specific protease 30, USP30

Recommended products

Rabbit Recombinant Monoclonal USP30 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27024-81
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

USP30 also known as ubiquitin specific peptidase 30 functions as a deubiquitinating enzyme. It has a mass of about 59 kilodaltons. This enzyme removes ubiquitin from protein substrates playing an important role in the regulation of protein degradation. USP30 is normally expressed in mitochondria where it participates in maintaining protein homeostasis by removing polyubiquitin chains.

Biological function summary

The deubiquitinating activity of USP30 helps maintain mitochondrial integrity. It is part of the endoplasmic reticulum-associated degradation (ERAD) complex that ensures the removal of misfolded proteins. Without USP30's activity cells may accumulate damaged proteins leading to cellular stress and dysfunction. This activity influences mitochondrial dynamics by counteracting the ubiquitination activities within cells.

Pathways

USP30 plays a significant role in the mitochondrial quality control pathway and the ubiquitin-proteasome pathway. It regulates Parkin-mediated mitophagy by removing ubiquitin from mitochondrial substrates impacting processes that involve protein turnover and energy balance. USP30 works in conjunction with proteins like Parkin and PINK1 to maintain mitochondrial health by controlling the balance between ubiquitination and deubiquitination.

Associated diseases and disorders

Deficiencies of USP30 associate with neurodegenerative diseases like Parkinson’s disease where impaired mitophagy and protein accumulation occur. It also connects with mitochondrial disorders as improper regulation of mitochondrial proteins by USP30 leads to energy production issues. In these conditions USP30's interaction with proteins Parkin and PINK1 becomes important for preventing cellular and mitochondrial dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749), expandable thumbnail

    Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749)

    USP30 Western blot staining using rabbit Anti-USP30 antibody

    Western blot: Rabbit Monoclonal [EPR27024-81] to USP30 ab314749 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 59 kDa in Wild-type A549 ab288558 cell lysates with no signal observed at this size in USP30 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: USP30 knockout A549 cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 59 kDa

    Observed band size: 59 kDa

  • Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749), expandable thumbnail

    Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    This blot was developed using a high sensitivity ECL substrate.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 58 kDa

    Exposure time: 180s

  • Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749), expandable thumbnail

    Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    This blot was developed using a high sensitivity ECL substrate.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human kidney tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Lane 4: Rat liver tissue lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

    Lanes 3 - 4: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 58 kDa

    Exposure time: 180s

  • Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749), expandable thumbnail

    Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    This blot was developed using a high sensitivity ECL substrate.

    All lanes: Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 58 kDa

    Exposure time: 180s

  • Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749), expandable thumbnail

    Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    This blot was developed using a high sensitivity ECL substrate.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: T47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

    Lane 7: U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 58 kDa

    Exposure time: 180s

  • Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749), expandable thumbnail

    Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-USP30 antibody [EPR27024-81] (ab314749) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: 293T transfected with siRNA specifically target USP30 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 58 kDa

    Exposure time: 180s

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Product protocols

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