Anti-USP31 + USP43 antibody [EPR26029-42] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-USP31 + USP43 antibody [EPR26029-42] - BSA and Azide free (AB320633)

This data was developed using ab320632, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody cross-reacts with overexpressed human USP31 (Q70CQ4) by WB In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-USP31 + USP43 antibody [EPR26029-42] (<a href='/en-us/products/primary-antibodies/usp31-usp43-antibody-epr26029-42-ab320632'>ab320632</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-his tag whole cell lysate at 2 µg

Lane 2:

293T cells transfected with a human USP43 expression vector containing a myc-his tag whole cell lysate at 2 µg

Lane 3:

293T cells transfected with a human USP1 expression vector containing a myc-his tag whole cell lysate at 2 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 123 kDa,36 kDa,15 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-USP31 + USP43 antibody [EPR26029-42] - BSA and Azide free (AB320633)

This data was developed using ab320632, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible. The identity of the higher MW band at approximately 250 kDa is unknown.

All lanes:

Western blot - Anti-USP31 + USP43 antibody [EPR26029-42] (<a href='/en-us/products/primary-antibodies/usp31-usp43-antibody-epr26029-42-ab320632'>ab320632</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 123 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-USP31 + USP43 antibody [EPR26029-42] - BSA and Azide free (AB320633)

This data was developed using ab320632, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lysate was freshly made and used for Western Blotting immediately to minimize protein degradation. The identity of the higher MW band at approximately 250 kDa is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-USP31 + USP43 antibody [EPR26029-42] (<a href='/en-us/products/primary-antibodies/usp31-usp43-antibody-epr26029-42-ab320632'>ab320632</a>) at 1/1000 dilution

Lane 1:

HCT 116 (human colorectal carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HCT 116 transfected with siRNA specifically targeting USP43 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 123 kDa,36 kDa

false

Exposure time: 180s