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AB236452

Anti-USP36 antibody

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(4 Publications)

Rabbit Polyclonal USP36 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP36.

View Alternative Names

KIAA1453, USP36, Ubiquitin carboxyl-terminal hydrolase 36, Deubiquitinating enzyme 36, Ubiquitin thioesterase 36, Ubiquitin-specific-processing protease 36

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-USP36 antibody (AB236452)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP36 antibody (AB236452)

2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with Flag-USP36 stained for USP36 (red) using ab236452 at 1/2000 dilution in ICC/IF. Flag (green). Right panel shows the merged images with DAPI.

Flow Cytometry - Anti-USP36 antibody (AB236452)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-USP36 antibody (AB236452)

Flow cytometric analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cell line labeling USP36 with ab236452 at 1/200 dilution (green) compared with a negative control with no primary antibody (blue).

Western blot - Anti-USP36 antibody (AB236452)
  • WB

Supplier Data

Western blot - Anti-USP36 antibody (AB236452)

All lanes:

Western blot - Anti-USP36 antibody (ab236452) at 1/2000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with control siRNA

Lane 2:

U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with USP36 siRNA

Lane 3:

Mouse MEF cell lysate

Lane 4:

Rat INS-1 cell lysate

Predicted band size: 123 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human USP36. The exact immunogen used to generate this antibody is proprietary information.

Q9P275

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP36 or Ubiquitin Specific Peptidase 36 functions as a deubiquitinating enzyme. It removes ubiquitin moieties from specific protein substrates influencing their stability and degradation. The enzyme weighs approximately 123 kDa. USP36 expresses in various tissues throughout the body with significant levels in the nucleolus. This protein involves in cellular regulation due to its role in controlling ubiquitination which is an important process in protein turnover.
Biological function summary

USP36 plays a role in regulating ribosomal RNA gene transcription and processing. It forms part of complexes that include nucleolar proteins facilitating nucleolar homeostasis. This regulation impacts cell growth and division linking USP36 to fundamental cellular activities such as ribosome biogenesis. By influencing these processes USP36 ensures that cells maintain proper protein synthesis rates necessary for growth and division.

Pathways

Changes in USP36 influence the ubiquitin-proteasome pathway and ribosome biogenesis pathway. USP36 interacts with proteins like FBL (Fibrillarin) and NPM1 (Nucleophosmin) key players in these pathways coordinating ribosomal RNA synthesis and nucleolar architecture. The protein supports the removal of ubiquitin from nucleolar substrates affecting their ability to respond to signaling events related to cell division and stress responses.

Disruption in USP36 activity has links to cancer particularly where nucleolar function is perturbed. It associates with tumorigenesis through interactions with proteins like MYC a well-known oncogene. Additionally aberrations in USP36 function contribute to neurodegenerative disorders through its involvement in protein aggregation drawing connections with ubiquitin-related proteins within cellular maintenance processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinase essential for the regulation of nucleolar structure and function (PubMed : 19208757, PubMed : 22902402, PubMed : 29273634). Required for cell and organism viability (PubMed : 19208757, PubMed : 22902402, PubMed : 29273634). Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability (PubMed : 19208757, PubMed : 22902402, PubMed : 29273634, PubMed : 36912080). Functions as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) (PubMed : 29274341). Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome : acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex (PubMed : 25775507). In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm (PubMed : 25775507). Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions (PubMed : 27445338). Deubiquitinates SOD2, regulates SOD2 protein stability (PubMed : 21268071). Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins (PubMed : 22622177). Promotes CEP63 stabilization through 'Lys-48'-linked deubiquitination leading to increased stability (PubMed : 35989368). Acts as a SUMO ligase to promote EXOSC10 sumoylation critical for the nucleolar RNA exosome function in rRNA processing (PubMed : 36912080). Binds to pre-rRNAs (PubMed : 36912080).
See full target information USP36
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 182:3594-3611 PubMed40194519

2025

Inhibition of DNA polymerase eta-mediated translesion DNA synthesis with small molecule sensitises ovarian cancer stem-like cells to chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Subhankar Bose,Priyanka Saha,Md Tanjim Alam,Bilash Chatterjee,Mrinmoy Sarkar,Amit Kumar Dixit,Deepak Kumar,Rakesh Kumar Pathak,Prem Prakash Tripathi,Amit Kumar Srivastava

Cell cycle (Georgetown, Tex.) 22:680-692 PubMed36305548

2022

miR-140-3p/usp36 axis mediates ubiquitination to regulate PKM2 and suppressed the malignant biological behavior of breast cancer through Warburg effect.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Yile Jiao,Chen Zhou,Xinyi Guo,Zhenru Wu,Qing Lv

Human cell 35:694-704 PubMed35133629

2022

USP36 promotes tumor growth of non-small cell lung cancer via increasing KHK-A expression by regulating c-MYC-hnRNPH1/H2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Deng,Maolin Wu,Jing Deng

The Science of the total environment 776:145950 PubMed33647641

2021

Lnc-HZ01 with m6A RNA methylation inhibits human trophoblast cell proliferation and induces miscarriage by up-regulating BPDE-activated lnc-HZ01/MXD1 positive feedback loop.

Applications

WB

Species

Human

Zhongyan Xu,Peng Tian,Jiarong Guo,Chenyang Mi,Tingting Liang,Jiayu Xie,Wenxin Huang,Mengyuan Dai,Weina Chen,Huidong Zhang
View all publications
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Product promise

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