AB229092

Anti-USP37 antibody

Name: Anti-USP37 antibody (ab229092) | Abcam
Brand: Abcam Limited
SKU: AB229092
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal USP37 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP37.

View Alternative Names

KIAA1594, USP37, Ubiquitin carboxyl-terminal hydrolase 37, Deubiquitinating enzyme 37, Ubiquitin thioesterase 37, Ubiquitin-specific-processing protease 37

1 Images

Supplier Data

Western blot - Anti-USP37 antibody (AB229092)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-USP37 antibody (ab229092) at 1/1000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Predicted band size: 110 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human USP37. The exact immunogen used to generate this antibody is proprietary information.

Q86T82

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP37 also known as Ubiquitin-Specific Protease 37 is an enzyme that functions as a deubiquitinase. Mechanically it removes ubiquitin from specific protein substrates therefore regulating their degradation by the proteasome. The protein has a mass of approximately 110 kDa. It expresses widely across various tissues with higher levels noticeable in testis brain and placenta which suggests a context-dependent role in different cell types.

Biological function summary

USP37 plays an important role in cell cycle regulation by stabilizing proteins that are key during this process. For example it stabilizes the cyclin A protein which is necessary for the transition between G1/S phases of the cell cycle. USP37 does not function alone; it interacts with other proteins and sometimes becomes part of complexes orchestrating both cellular growth and division by ensuring precise timing and progression within the cell cycle.

Pathways

USP37 is particularly integral to the cell cycle control and DNA damage response pathways. In the cell cycle pathway it closely interacts with Cyclin-dependent kinase 2 (CDK2) regulating transition checkpoints. In the DNA damage response pathway its interaction with proteins like ATR (ataxia telangiectasia and Rad3-related protein) helps maintain genomic integrity by modulating repair processes. These interactions illustrate how USP37 links mechanistic actions to larger functional pathways.

USP37 has implications in oncogenesis and neurodegenerative diseases. Its deregulation may contribute to the development of cancers by affecting the stability of proteins involved in cell proliferation evident in some breast and prostate cancers. In neurodegenerative diseases like Alzheimer’s USP37’s interaction with the protein Tau may influence neurofibrillary tangle formation a hallmark of the disorder. Understanding these connections aids in researching potential therapeutic targets and interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Deubiquitinase that plays a role in different processes including cell cycle regulation, DNA replication or DNA damage response (PubMed : 26299517, PubMed : 27296872, PubMed : 31911859, PubMed : 34509474). Antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) (PubMed : 21596315). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1 (PubMed : 27296872). Plays also an essential role beyond S-phase entry to promote the efficiency and fidelity of replication by deubiquitinating checkpoint kinase 1/CHK1, promoting its stability (PubMed : 34509474). Sustains the DNA damage response (DDR) by deubiquitinating and stabilizing the ATP-dependent DNA helicase BLM (PubMed : 34606619). Mechanistically, DNA double-strand breaks (DSB) promotes ATM-mediated phosphorylation of USP37 and enhances the binding between USP37 and BLM (PubMed : 34606619). Promotes cell migration by deubiquitinating and stabilizing the epithelial-mesenchymal transition (EMT)-inducing transcription factor SNAI (PubMed : 31911859). Plays a role in the regulation of mitotic spindle assembly and mitotic progression by associating with chromatin-associated WAPL and stabilizing it through deubiquitination (PubMed : 26299517).

See full target information USP37

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 13:2323-2341 PubMed37424824

2023

USP37 promotes angiogenesis and metastasis in colorectal cancer by facilitating β-catenin stability.

Applications

Unspecified application

Species

Unspecified reactive species

Dafeng Tong,Jie Yuan,Zhaoming Wang,Enda Yu,Jifu E

BMC molecular and cell biology 21:65 PubMed32928102

2020

Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFβ/SMAD2 axis in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhou Liu,Qing Yang,Kevin Chen Xiao,Thomas Dobleman,Shen Hu,Gary Guishan Xiao

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-USP37 antibody

Western blot - Anti-USP37 antibody (AB229092)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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