AB72244

Anti-USP38 antibody

Name: Anti-USP38 antibody (ab72244) | Abcam
Brand: Abcam Limited
SKU: AB72244
Rating: 3 (2 reviews)

(2 Reviews)

(4 Publications)

Rabbit Polyclonal USP38 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human USP38.

View Alternative Names

KIAA1891, USP38, Ubiquitin carboxyl-terminal hydrolase 38, Deubiquitinating enzyme 38, HP43.8KD, Ubiquitin thioesterase 38, Ubiquitin-specific-processing protease 38

2 Images

Unknown

Immunoprecipitation - Anti-USP38 antibody (AB72244)

Immunoprecipitation/ Western Blot of USP38.
Lane 1 : ab72244 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

ab72244 at 1μg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-USP38 antibody (ab72244)

Predicted band size: 117 kDa

false

Unknown

Western blot - Anti-USP38 antibody (AB72244)

All lanes:

Western blot - Anti-USP38 antibody (ab72244) at 0.1 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 50 µg

Lane 2:

Whole cell lysate from Hela cells at 15 µg

Lane 3:

Whole cell lysate from Hela cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 117 kDa

Observed band size: 117 kDa,31 kDa,68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human USP38. The exact immunogen used to generate this antibody is proprietary information.

Q8NB14

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP38 or Ubiquitin Specific Peptidase 38 is a deubiquitinating enzyme with a pivotal role in the regulation of protein degradation. It maintains cellular protein levels by removing ubiquitin from specific substrates thereby controlling their stability and function. USP38 has an estimated mass of approximately 111 kDa. Expression of USP38 occurs in various tissues with notable expression in the brain and immune system indicating its broad relevance to many physiological processes.

Biological function summary

USP38 participates in intricate cellular processes by modulating the ubiquitin-proteasome system. This protein is not generally described as part of a large complex acting predominantly as an independent agent in deubiquitination. Its activity affects cell cycle progression and apoptosis by selectively regulating substrate proteins involved in these functions. Such biological roles highlight its importance in cellular health and proliferation.

Pathways

USP38 interacts with critical signaling pathways including the NF-kB and p53 pathways. Within the NF-kB pathway USP38 modulates immune and inflammatory responses through its deubiquitinase activity potentially affecting proteins like IkB or other signaling regulators. In the context of the p53 pathway USP38 may influence cell cycle regulation and apoptosis through interacting proteins such as MDM2 a ubiquitin ligase associated with p53 degradation.

Aberrant USP38 function has links to cancer and neurodegenerative diseases. In cancer it influences tumor progression possibly by interacting with p53 and MDM2 affecting cell regulation and apoptosis. In neurodegenerative diseases such as Alzheimer's altered ubiquitination processes involving USP38 may disturb protein degradation contributing to disease pathology. These associations highlight UNC38 as an important target for potential therapeutic strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Deubiquitinating enzyme that plays a role in various cellular processes, including DNA repair, cell cycle regulation, and immune response (PubMed : 22689415, PubMed : 30497519, PubMed : 31874856, PubMed : 35238669). Plays a role in the inhibition of type I interferon signaling by mediating the 'Lys-33' to 'Lys-48' ubiquitination transition of TBK1 leading to its degradation (PubMed : 27692986). Cleaves the ubiquitin chain from the histone demethylase LSD1/KDM1A and prevents it from degradation by the 26S proteasome, thus maintaining LSD1 protein level in cells (PubMed : 30497519). Plays a role in the DNA damage response by regulating the deacetylase activity of HDAC1 (PubMed : 31874856). Mechanistically, removes the 'Lys-63'-linked ubiquitin chain promoting the deacetylase activity of HDAC1 in response to DNA damage (PubMed : 31874856). Also acts as a specific deubiquitinase of histone deacetylase 3/HDAC3 and cleaves its 'Lys-63'-linked ubiquitin chains to lower its histone deacetylase activity (PubMed : 32404892). Regulates MYC levels and cell proliferation via antagonizing ubiquitin E3 ligase FBXW7 thereby preventing MYC 'Lys-48'-linked ubiquitination and degradation (PubMed : 34102342). Participates in antiviral response by removing both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of Zika virus envelope protein E (PubMed : 34696459). Constitutively associated with IL-33R/IL1RL1, deconjugates its 'Lys-27'-linked polyubiquitination resulting in its autophagic degradation (PubMed : 35238669).

See full target information USP38

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 18:e1010366 PubMed36288387

2022

The feedback loop of METTL14 and USP38 regulates cell migration, invasion and EMT as well as metastasis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Huang,Weimin Zhou,Chao Hao,Qiuming He,Xinhua Tu

Folia neuropathologica 59:359-371 PubMed35114776

2022

USP38 might be a new therapeutic target for glioma via regulation of cancer cell metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Hu,Ke-Ni Wang,Hai-Rui LingHu

Viruses 13: PubMed34696459

2021

USP38 Inhibits Zika Virus Infection by Removing Envelope Protein Ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchong Wang,Qin Li,Dingwen Hu,Daolong Gao,Wenbiao Wang,Kailang Wu,Jianguo Wu

Science advances 4:eaar2824 PubMed29732405

2018

Broad and diverse mechanisms used by deubiquitinase family members in regulating the type I interferon signaling pathway during antiviral responses.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxiang Liu,Yaoxing Wu,Yunfei Qin,Jiajia Hu,Weihong Xie,F Xiao-Feng Qin,Jun Cui

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP38 antibody

Immunoprecipitation - Anti-USP38 antibody (AB72244)

Western blot - Anti-USP38 antibody (AB72244)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise